甲苯胺

METHODS: Twenty pationts with spinal tuberculosis were treated by anterior partial vertebrectomy of the diseased vertebrae, iliac grafting and internal fixation via anterior of posterior approach.

20例脊柱结核患者，施行病灶部分切除、髂骨植骨、前路或后路内固定手术，术后将切除的大块病灶骨用MMA包埋、不脱钙骨切片、甲苯胺蓝染色，观察结核特征性病灶在整块骨中的分布情况。

In the same reactor, telon fast green 5G was synthesized through condensation and sulfonation processes with 1,4,5,8-hydroxyanthraquinone and p-toluidine as main raw materials.

由1，4，5，8—四羟基蒽醌和对甲苯胺为主要原料，在一个反应器内同时完成了缩合和磺化两步单元反应，合成酸性蒽醌绿染料，并对其反应条件对产物收率的影响进行了研究，确定了最佳合成工艺条件(反应时间16 h、反应温度120℃、原料的配比1：2.2)。

Toluidine blue staining procedure showed that the neural complex consisted of cerebral ganglion, neural gland and loose connective tissue; the cerebral ganglion was divided into three parts as cerebral cortex, medullary layer and transitional zone; the neural gland was composed of glandular lobules, closely apposing at ventral side of the cerebral ganglion; the neurons and nerve fibers were colored deeply, making a distinct contrast with the background, while neither the neurons nor the nerve fibers were distinctly colored by Golgi-argentraffin staining procedure.

甲苯胺蓝染色法显示，神经复合体由神经节、神经腺和疏松结缔组织组成；神经节分为皮质部、髓质部和过渡带；神经腺位于神经节背侧面，由腺小叶组成；神经细胞呈深蓝色，神经纤维呈浅蓝色，与背景反差较大。Golgi镀银法显示的结果不理想，神经细胞和神经纤维着色不明显。

The culture was treated with lutein of different concentrations 10^(-8mol/L、10^(-7)mol/L、10^(-6)mol/L, respectively. The culture cells were fixed and were stained for tartate-resistant acidic phosphatase after 7d. The formation of osteoclasts was quantified by counting the number of TRAP(superscript +) multinuclear cells. The percentage of bone resorbed surface, TRAP activity and the expression of receptor activator of NF-KB mRNA were analysed. The appearance of reduced mice osteoclasts was typical.

受试细胞分为对照组、叶黄素低剂量组10^(-8mol/L、叶黄素中剂量组10^(-7mol/L和叶黄素高剂量组10^(-6mol/L，并设立空白对照组。7 d后取细胞玻片进行抗酒石酸酸性磷酸酶染色，观察破骨细胞并计数；取骨片进行甲苯胺蓝染色，光镜下统计骨吸收陷窝面积；测量抗酒石酸酸性磷酸酶活性以及破骨细胞表面NF-κB活化受体mRNA表达量。

The coverslip with differentiated cells were fixed at different timepoints and used for toludine blue or inmunohistochemical dying Meanwhile, different doses of antibody to Fibronectin were added to observe aggregating phenomenon.

用2，3传代细胞按一定比例种植于24孔板内，加入TGF-β1 1，5，10ng／mL，分别于不同时间点取材固定，用于甲苯胺兰组织学染色及抗纤维连接蛋白抗体免疫组化染色。

The rats were killed by cutting theirnecks and physical blood was taken for preparing serum, interleukin 2 (IL-2) was detected by radioimmunoassay ; the spleen was taken for measuring ConA-induced reaction of lympho- cyte proliferation and induced production of IL-2; and the activity of induced production of spleen's IL-2 was determined by the means of cell proliferation of mouse thymus; the skin and trachea were taten for slice with paraffin wax, dyeing with methybenzoamine blue so as to survey mast cells .

将大鼠断头留取躯干血制备血清，用RIA法检测白细胞介素2（IL-2）；取脾脏行ConA诱导的淋巴细胞增殖反应测定和IL-2诱生；并用小鼠胸腺细胞增殖法检测脾IL-2诱生活性；取皮肤和气管做石蜡切片、甲苯胺兰染色检测肥大细胞，结果显示：应激组较对照组脾淋巴细胞增殖降低，cpm分别为9466±3.17和24019±3879（P.001）；MC明显减少，分别为8.6±3.0/HP和25.4±3.7/HP（P.001），前组形态异常较其甚（MC多较小，呈多角或不规则状），且组织中可见分散的崩解颗粒。

The lung was fixed in 10% formalin solution and embedded in paraffin for Haematoxyffin-eosin staining to observed the form of embolisms, Masson staining to assess collagen and fibrin/fibrinogen distribution and Toluidine blue staining was to observe mast cell distribution.

以10%甲醛固定肺组织，制成组织切片，行苏木精－伊红染色观察血栓的形态，Masson染色观察栓塞血管的胶原纤维分布，甲苯胺蓝染色观察血管栓塞处肥大细胞分布。

Study in vitro The chondrocytes of SD rat were isolated from epiphyses by collagenase Ⅱ and the cultured chondrocytes were confirmed by HE, toluidine blue, alcian blue staining, and by IHC with anti-collagen type Ⅰ and Ⅱ.

体外实验 SD大鼠骺软骨细胞分离培养，HE、甲苯胺蓝、标准阿尔新蓝染色及Ⅰ、Ⅱ免疫组化染色鉴定，绘制细胞生长曲线。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

The nerve axon regeneration length was measured 2 weeks after operation. The effects of peripheral nerve regeneration were evaluated by neural electrophysiology, the recovery rate of triceps surae muscular tension and weight and histological assessment 16 weeks after operation. Results All the animals survived till the end of experiment.

术后行大体观察；术后2 周测量神经轴突生长距离；术后16 周行电生理检测，计算术侧坐骨神经运动传导速度恢复率、术侧小腿三头肌收缩力恢复率及湿重恢复率，并行移植神经吻合中段HE 和半薄切片甲苯胺蓝染色观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。