甲苯胺

The histological staining showed that toluidine blue, safranin O and anti-collagen II immunohistochemistry staining were positive.

组织学观察显示，三维多孔支架甲苯胺蓝染色、番红O染色、Ⅱ型胶原免疫组织化学染色均呈阳性。

The effect of Alcian blue Safranin O staining was the best, which was applicable to identification of the mast cell of A. Japonica. The improved toluidine blue staining was not applicable.

AB/SO染色法效果最好，适用于日本鳗鲡肥大细胞的鉴定；改良甲苯胺蓝染色法不适合于日本鳗鲡肥大细胞的鉴定。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化，于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色，以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下，于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色阳性，诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好；移植皮下后第4周取材可见外形呈类骺软骨样组织块，行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色，镜下观察可见大量软骨细胞及类软骨陷窝样结构，植入的海藻酸钙微球周围组织无明显炎症反应。

In this article we researched the effect of ο-toluidine on the growth of silver nanorods .

探讨了邻甲苯胺在制备银纳米棒中的作用。

At day 7, 14 and 21 after induction respectively, Toluidine blue staining and immunocytochemical staining were performed to detect differentiation.

在诱导第7，14，21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups.

实验组甲苯胺蓝染色和Ⅱ型胶原染色为阳性，对照组和空白组均为阴性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。