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A transformant line obtained by transfection of NTH3T3 cells with c-Ha-T24-ras gene was treated with 5mmol/L benzamide,an inhibitor of PARP enzyme for 4 weeks.Some changes in cellular properties of the transformant line were observed.

用 5mmol/L PARP抑制剂苯甲酰胺处理经c-Ha-T24-ras活化癌基因转染得到的转化细胞系,处理4周后发现转化细胞系的某些细胞生物学特性发生了改变,呈现出正常细胞的某些特性。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后,检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况,结果如下:BA诱导的基因删除可发生于NIH3T3转化细胞系,但不能发生于HeLa转化细胞系;位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的;外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

This method was applied to determine aromatic amines in textiles with recovery of 95%~98% for o-toluidine and 78%~85% for benzidine.

将本方法用于测定纺织品中的芳香胺化合物,邻甲苯胺和联苯胺的回收率分别为95%~98%和78%~85%。

The conditions for HPLC to detect endogenous polyamines were optimized, that is, benzoylation for 30min at 37℃, detection wave length at 230nm, elution speed at 0.7ml·min〓 Using the above method, endogenous polyamines of calli in Citrus were analyzed.

优化了植物多胺的HPLC测定条件,确定在37℃苯甲酰化反应30min,检测波长230nm,流速0.7ml·min〓效果最佳。

The mixed trimeric compounds containing one ferrocene unit, N,N'-bis(2-hydroxy-5-substituted benzylidene)ferrocenylmethylenediamine, have been synthesized directly through the reaction between 1 mol of ferrocenecarboxaldehyde and 2 mol of 5-substituted salicylaldehyde.

通过甲酰基二茂铁与取代水杨醛和氨反应,直接合成了含一个二茂铁的混合三聚产物, N,N'-二[2-羟基-5-取代苄烯]二茂铁甲二胺。

In this thesis, a reductive amination was used to prepare compound 16 with the boronic acid moiety. The moiety can provide covalent interaction with sugars.

本论文中,合成化合物16,是将化合物15与2-甲醯苯硼酸用还原胺化法而得到;其含有硼酸部分,可以与醣类分子以共价键键结。

2 Mm were compared using the Lipophilic Hydrophilic Ratio concept which was defined on the basis of the Washburn equation, and their surface free energy component s were also estimated using the Washburn equation and van Oss-Chaudhurry-Good theory with n-hexane,α-bromonaphthalene, formamide and water as probe liquids.

2mm无烟煤、锰砂和石英砂滤料的润湿性;同时以正己烷、1-溴萘、甲酰胺和去离子水为探针液,用Washburn方程和vanOss-Chaudhurry-Good理论对滤料表面自由能成分进行了估算。

A salt or ester of carbamic acid, especially one used as an insecticide.

丙烯酰胺、甲代烯丙基磺酸钠等为原料,采用溶液聚合法合成出兼含膦酰基和磺酸基的四元聚合阻垢剂PMAAS,。。。

The results of the analysis of the pectin extracted show ed that the degree of free and Salted forms, the degree of amide substitution,and the degree of esterification of carboxy groups were 85.1%, 1.1%, and 13.8%,...

我们对蚕粪果胶的分析表明,其羧基的游离及成盐程度、酰胺化程度、酯化程度分别为85.1%、1.1%和13.8%,甲氧基含量、半乳糖醛酸含量分别为1.79%和81.5%;特性粘度为267。

A homogeneous redox initiator system with ceric ammonium nitratein N,N-dimethyl-formamidewas reported in this paper.

研究了均相氧化还原体系硝酸铈铵/N,N-二甲基甲酰胺在无外加还原剂的条件下,引发丙烯酸甲酯,苯乙烯和甲基丙烯酸甲酯等烯类单体的聚合。

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The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因,如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务,你就做你的事好了。

Who is in possession of this?

这是谁的?