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Methylated Micrococcus luteus DNA was prepared with different DNA methylation level by using Hha I DNA methylase and Sephadex G-100 gel filtration.

本文用Hha I DNA甲基化酶为工具酶,制备了不同甲基化水平的Micrococcus IuteusDNA,再用Sephadex G-100凝胶过滤将甲基化了的M.L。

Additionally, the type of methyaltion changes in plants after seed and seedling irradiation were different.

另外,甲基化和去甲基化变化类型的分析也表明种子和幼苗辐射后发生甲基化变化的趋势也不相同。

Methods Naloxone was synthesized starting from oxidation of thebaine, followed by hydrogenation, acylation, cyanidation, O-demethylation, hydrolyzation and N-allylation. The alternative method of O-demethylation, methane sulfonic acid/DL-methionine and hydrobromic acid were investigated.

在以蒂巴因为原料,经氧化、氢化、酰化、氰化、去氧甲基、水解、N-烯丙基化得到纳洛酮的合成路线中,分别采用甲磺酸/DL-甲硫氨酸和氢溴酸代替三溴化硼进行去氧甲基化和后续水解反应。

However, analysis of DNA methylation level indicated that the total DNA methylation levels were not significantly different between diploid and autotetraploid. Autotriploid showed a trend of low DNA methylation level. It implied that the alterations of DNA methylation levels were not linear with the autopolyploidy level in watermelon.

有趣地是,二倍体与四倍体西瓜相比DNA甲基化水准却未发生大规模的调整,而三倍体西瓜显示出较为明显的低DNA甲基化水准趋势,表明西瓜同源多倍化过程中其基因组DNA甲基化水准变化与其倍性不成线性关系。

Ib was synthesized by condensation of phthalic anhydride with 2-methylresorcinol in fused AlCl〓/NaCl (5∶1), followed by selective acetylation, methylation, NBS bromination and condensation with sodium ethoxide.

邻苯二甲酸酐在熔融的三氯化铝-氯化钠(5:1)中和-2,6-二羟基甲苯环合得1,3-二羟基-2-甲基蒽醌〓后经选择性乙酰化、甲基化、NBS溴化、乙醇钠缩合得1-甲氧基-2-乙氧甲基-3-羟基蒽醌。

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后,研究固定化酶降解甲基对硫磷,从不同材料的膜载体固定有机磷水解酶的比较中,发现亲水膜比疏水膜适合作固定化酶载体;在此基础上,将有机磷水解酶固定在聚醚砜微滤膜上,并制成酶膜生物反应器,用于降解甲基对硫磷;戊二醛化学交联法固定时,酶液与10%牛血清白蛋白溶液的比例在3:1~2:1,与交联剂的比例在7:2~7:4范围内,固定化效果较好,并发现牛血清白蛋白和膜载体有降低酶活损失的作用;将固定化的酶膜装于酶膜反应器降解甲基对硫磷,实验结果表明:反应器的降解速率与固定化酶量呈正比,也随底物浓度的增加而加快,当流速低时,降解速率随流速增大而加快,到7ml/min以上时,降解速率不随流速脑黾佣洌到獾淖畹团ǘ任?

The fluorescence from each bound product is measured, and the readout can be used to determine whether a CpG site is fully methylated, semi-methylated, or unmethylated.

对来自每一个结合产物的荧光进行测量,结果可以用于确定一个CpG位置是完全甲基化,半甲基化,还是未甲基化。

Methylation analysis showed there were different levels of methylation in Zizania latifolia, but high level of methylation in rice

甲基化分析表明,反转座酶序列在菰中有不同程度的甲基化,但在水稻中均为高度甲基化。

Although PRMT1 methylates nuclear orphan receptor HNF4,whether TR3,another orphan receptor,is methylated by PRMT1 and how the methyltransferase activity of PRMT1 is affected by nuclear receptor remain largely unknown.

虽然PRMT1可以甲基化核受体HNF4,但孤儿受体TR3能否发生甲基化以及PRMT1的甲基化酶活性是否被核受体调控等问题还不清楚。

Because a part of hydroxyl group in methyl cellulose were substituted by trimethylsilyl group , the product could be dissolved in amounts of common organic solvents, such as chloroform, xylene and so on.

通过甲基纤维素与六甲基二硅亚胺的反应,成功地实现了对水溶性物质甲基纤维素醚三甲基硅化的改性;红外光谱验证了反应的发生,核磁共振氢谱确定了三甲基硅甲基纤维素的取代度;纤维素醚上的部分羟基被三甲基硅化后取得了在普通溶剂中的良好的溶解性,且随着取代度的增加,在非极性溶剂中的溶解性逐渐变好;通过热失重实验,对其热稳定性进行了测试。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?