甲基化的

The method has the advantages of high analysis speed and high sensitivity, can detect micro-target DNA methylation, and can carry out the joint detection of the polygene methylation.

具有分析速度快、灵敏度高，能检测微量靶DNA甲基化，可同时进行多基因甲基化的联合检测。

To find methylated genes, researchers usually turn to a technique called bisulphite sequencing, which chemically changes normal cytosine to thymidine (T; another DNA base), while leaving methylated cytosines unchanged.

研究者通常借助于亚硫酸氢盐测序技术检测甲基化的基因，它通过化学反应使正常胞嘧啶转变为胸腺嘧啶，而对甲基化的胞嘧啶无作用。

Other methyl nucleophiles includingMeMgBr, MeCeCl2, MeTiCl3, Me3Al, Me3ZnMgCl, MeMnClwere tried, but in all these cases no detectable amount of exo-C8-methylated product was obtained. Although protecting C3-carbonyl as 1,3-dioxolanes could be achieved, the serious enolization of C8-carbonyl was still problematic. Converting C9-furan ring to α,β-butenolide did depress the enolization, but desired C8-methylation was completely replaced by migration of the double bond of the butenolide.

在本论文的早期阶段，主要着眼于解决二环[3.2.1]-C8-羰基加成甲基所遇到的C9-呋喃环差向异构化和C3与C8两个羰基之间化学选择性的问题：通过引入不同金属降低甲基试剂的碱性并没有取得甲基化的成功；通过不同的方法将C3-羰基掩蔽，但是C8-羰基的烯醇化依然无法抑制；虽然通过将呋喃环转化为α，β-不饱和-γ-丁内酯可以抑制烯醇化，但内酯环上的双键迁移又成了新的竞争反应。

First, the preparation of the aminom ethylation of tannin using its electrophilic reaction activity of C-6, C-8 in ring-A; then process the quaternization base on the aminom ethylation; last, progress the sulfonation by use of the activity of phenolic hydroxyl hi ring-B.

首先利用单宁A环的C—6、C—8的亲电反应活性制备单宁胺甲基化产物；然后在胺甲基化的基础上进行季胺盐化；最后利用单宁B环的酚羟基的活性进行磺化。

Among all, deleted in liver cell (DLC-1) gene was found to play an important role in hepatoma in 1998, and after that hypermethylation on the promoter of the DLC-1 gene was found in many other cancers, suggesting that epigenetic modification is another way to control the carcinogenesis. Another gene, Endothelin type B receptor, was found to cause megacolon syndrome by accident while searching the cause of white spot in mouse, and was also found hypermethylation on its promoter in some cancer cells.

在此中，DLC-1基因自从1998年后被发现在肝癌的演化上扮演重要的角色后，在许多其他的癌症上也发现此基因上位於promoter的CpG islands有过度甲基化的现象，推论DLC-1基因的过度甲基化是一种经由epigenetic modification调节细胞癌化的方式。

The functions and interactions of DNA methylation regulating factors, the establishment, mantainace and removement mechanisms of DNA methylations are reviewed in this paper.

DNA转葡糖基酶有去甲基化活性从而减轻基因沉默。文章综述了以上植物DNA甲基化调控因子的生物学功能及其之间的相互作用和近年来的研究进展，以更好的理解DNA甲基化的建立、保持和去除的机制。

It is well known that isorhamnetin is used in clinic to cure coronary artery diseases, rhamnetin is used as a fine anti-inflammatory, and these medicines are just such methylated products.

本课题通过对槲皮素甲基化的研究，力求得到五个不同位置的甲基化反应产物。

Bisulfite modification: A total of 1μg of genomic DNA, obtained from culture cells or tissues, was modified by sodium bisulfite to convert unmethylated cytosines to uracils.

2亚硫酸氢钠修饰取1μg上述DNA经亚硫酸氢钠处理，将非甲基化的胞嘧啶转变为尿嘧啶，而甲基化的胞嘧啶保持不变。

Promoter methylation of hTERT is a regular event in uveal melanoma.

葡萄膜黑色素瘤中 CpG 岛甲基化的启动子：通过启动子超甲基化使肿瘤相关基因失活是各种肿瘤发展过程中的一种普遍核外遗传现象。

In this thesis, based on a recent genome-scale dataset of DNA methylation in human brain tissues, we developed a classifier for predicting methylation status of CpG islands using a Support Vector Machine. Nucleotide sequence contents, transcription factor binding sties and Alu repeats are used as features for the classification. The method achieves accuracy of ~85% on the brain data.

本文的主要工作就是基于人脑测得的DNA甲基化数据，挖掘DNA序列特征，用支持向量机的方法实现了预测CpG岛甲基化的目标，得到了85%的预测正确率，同时还验证了所选的三类特征对于CpG岛甲基化的指导作用，它们分别是DNA序列组合、转录因子结合位点TFBS和短重复序列Alu。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。