甲基化的

Results: There was a clear bimodal distribution of 80 microsatellite instability-high tumours according to the number of methylated promoters, with no tumours showing 3/5 methylated loci.

结果：根据启动子甲基化数目，80例高度微卫星不稳定的肿瘤出现明显的双峰分布，没有肿瘤是3/5的位点甲基化。

As an important transcription factor, methyl-CpG binding domain protein1 (MBD1) mediated transcription-suppressing course and might cause descentexpression of numerous tumor suppressor genes, or even result in devitalization ofthose genes.

作为一个重要的转录调控因子，胰腺癌中甲基化 CpG 结合域蛋白 1(methyl-CpG binding domain protein 1 ，MBD1)介导的甲基化转录抑制作用可能是造成众多抑癌基因转录表达下降，以至失活的重要原因。

Statistical evaluation was performed by using x~2 test and Fishers exact test for enumeration data and independent t test for measurement data to assess relationship of methylation between pancreatic neoplasm.

在从胰腺炎、IPMN至胰腺癌的进程中，NPTX2基因高甲基化阳性率逐渐加深，提示NPTX2基因可能参与胰腺癌进展，其高甲基化导致的基因沉默可能是导致胰腺癌的原因之一。

Objective To detect the expression of CD70 in peripheral T lymphocytes of patients with systemic lupus erythematosus and the effect of azacitidine, an inhibitor of DNA methylation, on it. Methods Blood samples were obtained from 10 patients with active SLE （SLEDAI score ≥5）, 10 patients with nonactive SLE （SLEDAI score 5） and 10 normal human controls.

目的 通过研究共刺激分子CD70在SLE患者外周血T淋巴细胞的表达水平，以及DNA甲基化抑制剂氮杂胞苷对正常人外周血T淋巴细胞表达CD70分子的影响，探讨DNA甲基化在SLE患者外周血T淋巴细胞表达CD70分子中的调控作用。

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

To investigate the characteristics of arsenic methylating metabolism in population in coal-burning type arsenism endemic area and in control area, and to explore the difference between the two population.

目的 描述燃煤型砷中毒病区人群与非病区人群砷甲基化代谢的特征，比较两区人群砷甲基化代谢水平的差异。

Recent progress of dimethyl carbonate which acted as methylating reagent in methylation reaction s that took place on carbon, oxygen, nitrogen and sulfur atoms was reviewed.

综述了碳酸二甲酯作甲基化试剂在碳、氧、氮和硫原子上进行甲基化反应的研究进展，提出了今后研究工作的发展方向。

cDNA microarray was an effective and useful method to survey gene expression profiles in pancreatic carcinoma. MBD1、EDG1 and its hyper methylating modulation may play an important role in the pathogenesis of pancreatic cancer, among which the high expression of MBD1 in pancreatic cancer would be the key modulating point in the down regulation of multiple methylation related suppression genes transcription.

cDNA微列阵技术为胰腺癌相关基因表达谱的分析提供了有力的工具，MBD1、EDG1等基因过甲基化表达调控可能在胰腺癌发病机制中起着关键作用，而MBD1在胰腺癌中高表达可能是多个甲基化相关抑癌基因转录表达下降的关键调控点。

MBD1、EDG1 and its hyper methylating modulation may play an important role in the pathogenesis of pancreatic cancer,among which the high expression of MBD1 in pancreatic cancer would be the key modulating point in the down regulation of multiple methylation related suppression genes transcription.

cDNA 微列阵技术为胰腺癌相关基因表达谱的分析提供了有力的工具，MBD1、EDG1等基因过甲基化表达调控可能在胰腺癌发病机制中起着关键作用，而MBD1在胰腺癌中高表达可能是多个甲基化相关抑癌基因转录表达下降的关键调控点。

In this experiment, we associated themethylation-sensitive single-strand conformation polymorphism analysis withlaser-induced fluorescence capillary electrophoresis to set up a new method which isfast separation, high sensitivity, and automated for detection of DNA methylation.

本研究通过甲基化敏感的单链构象分析法与毛细管电泳技术相结合建立一种高效、灵敏、自动化的DNA甲基化检测方法。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。