甲基化的

Technologies for determining methylation patterns in cancer cells, Methyl Light and COBRA, both of which were based on the deamination of methylated cytosines—which converts these cytosines to uracil residues—and identification of the converted sequence by analytical methods.

在上世纪80年代期间，研究的主要方法是PCR和克隆，两者都不涉及甲基化内容，因此很快就被弃用了。90年代，Laird开发了两类技术去检测肿瘤细胞中的甲基化模式，MethylLight和COBRA，两者都是以甲基化胞嘧啶的脱氨作用（将胞嘧啶转化为尿嘧啶残基）以及通过分析方法鉴定修改的序列为基础。

Veratrum aldehyde synthesis of many drugs intermediates, methyl aldehyde method for raw materials in Champlain, after dimethyl sulfate methylation available to 20% sodium hydroxide as the solvent, 10-11 in the PH value of the under the conditions of synthesis; microwave irradiation is the use of microwave radiation heating to replace the traditional approach to synthesis of a product of methylation reactions, this method does not require solvents, and less by-products, but still need to use 15% sodium hydroxide to adjust pH.

藜芦醛是许多药物合成的中间体，甲基化法以香兰醛为原料，经过硫酸二甲酯甲基化可得，以20%氢氧化钠为溶剂，在PH值为10-11的条件下合成；微波辐射法是利用微波辐射代替传统加热方式进行甲基化反应合成产物，这种方法不需要溶剂，并且副产物少，但仍需要用15%氢氧化钠来调节酸碱度。

In the NPC cell lines,loss of BLU expression correlated with hypermethylation of the 20 CpG sites in the 5′ region of BLU(approximately -292bp to -23bp upstream the translation start site)as revealed by bisulphite sequencing. Expression of the gene was restored aftertreatment with 5-aza-2-deoxycytidine in CNE2, suggesting that repression of BLUtranscription might at least be partially mediated by promoter methylation.

结果显示，BLU基因翻译起点ATG上游-292--23bp区间内的20个CpG位点在5例细胞系中均被完全或者部分甲基化，与基因的表达缺失呈负相关关系；去甲基化试剂5-Aza-CdR处理CNE2后基因表达部分恢复，说明甲基化至少是引起BLU基因转录抑制的部分原因。

Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel.

结果表明，3个组织甲基化水平存在一定差异，并在两自交系中表现出相同的变化趋势，即雌穗苞叶的甲基化敏感扩增多态性比率和全甲基化率最高，穗位叶次之，雄穗最低。

Ethylation specific PCR analysis also revealed that the BLU promoter was highlymethylated in 74％(17 of 23) of primary tumors where the gene was downregulated,whereas only 2 of 9 non-neoplastic nasopharyngeal epithelia exhibitedhypermethylation in the BLU promoter region.

SP分析的结果表明，在23例表达下调的鼻咽癌组织中，17例(74％，17／23)标本的BLU基因发生了异常甲基化，而9例非癌对照中仅有2例甲基化，说明启动子甲基化在BLU基因的转录失活中具有重要作用。

The alternative method of O-demethylation,methane sulfonic acid/DL-methionine and hydrobromic acid were investigated.

方法在以蒂巴因为原料，经氧化、氢化、酰化、氰化、去氧甲基、水解、N-烯丙基化得到纳洛酮的合成路线中，分别采用甲磺酸/DL-甲硫氨酸和氢溴酸代替三溴化硼进行去氧甲基化和后续水解反应。

In this article, high DS and low viscosity carboxymethyl starch was prepared in aqueous media through the reaction between corn starch with acid treatment and etherifying agent, the effect of acid treatment time, liquor/material ratio, the amount of sodium hydroxide, the amount of monochloroacetic acid, etherification time, and reaction tempera.

以玉米淀粉为原料，采用酸化-醚化复合变性的方法在水相中制备高取代度低黏度羧甲基淀粉，探讨了酸化时间、液固比、NaOH用量、ClCH2COOH用量、醚化反应时间、反应温度对羧甲基化过程的影响，研究发现，对原淀粉进行酸化，可以在不增加其它反应试剂用量的基础上提高产物的取代度，但是酸化时间不应过长。

For The Methylation Assay, the readout is called beta, which is calculated from the fluorescent signal ratio of Cy3 to Cy 5, where all green signal means unmethylated and all red, fully methylated.

甲基化分析的结果称作β，是以荧光信号Cy3于Cy5之比计算得出的结果，如果全部都是绿色信号，表示未甲基化，而全为红色则表示完全甲基化。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析，结果表明，甲基化程度存在差异的位点46个，其中18个位点为甲基化或半甲基化位点，推测橘红色闭壳肌的发生可能与这些位点有关，它们调控了该性状的表达。

The effect of low temperature treatment on DNA methylation level decrease revealed some variety differences. The DNA methylation level in stem apices of the spring radish cultivar'Duanye 13'was somewhat easy to be decreased by low temperature treatment, but that of the winter radish cultivar'Yidianhong'did not decrease when the germinated seeds were treated at 10℃ for 10 days.

萌动种子低温处理降低DNA甲基化水平的效果在品种间存在一定差异，春性品种'短叶13'其DNA甲基化水平比较容易被降低，而冬性品种'一点红'的萌动种子在10℃下处理10d尚不能降低其DNA甲基化水平。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。