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Methods 4-Hydroxy- benzonitrile was treated with sodium hydrogen sulfide and anhydrous magnesium chloride in dimethyl formamide to give thioamide, which was then directly cyclized with ethyl 2-chloroacetoacetate without separation to give ethyl 2-(4-hydroxyphenyl)-4-methylthiazole-5-carboxylate(2) in one-pot; then 2 was formylated with Duff reaction adopting hexamethylenetetramine in trifluoroacetic acid to give ethyl 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-5-carboxylate(3); finally, the target compound was obtained by the treatment of 3 with hydroxylamine hydrochloride and sodium formate in formic acid.

采用&一勺烩&方法,以4-羟基苯甲腈为起始原料,首先与硫氢化钠和无水氯化镁在N,N-二甲基甲酰胺中反应,所得中间体不经分离,直接加入2-氯乙酰乙酸乙酯进行环合反应,得到2-(4-羟基)苯基-4-甲基-5-噻唑甲酸乙酯(2);然后通过六亚甲基四胺/三氟乙酸进行Duff反应,得到2-(3-甲酰基-4-羟基)苯基-4-甲基-5-噻唑甲酸乙酯(3);再经盐酸羟胺/甲酸/甲酸钠体系脱水得到目标化合物。

Also, the type of dispersion of four gasliquid-liquid systems: air-decane-aqueous solution of sodium sulfite and surfactant, airmixture of decane and dodecene-aqueous solution of rhodium complex, ligand, and surfactant, air-mixture of decane, dodecene and tridecyl aldehyde-aqueous solution of rhodium complex, ligand and surfactant, and air-mixture of decane and dodeceneaqueous solution of trisodium salt of tri (m-sulfophenyl) phosphine, and surfactant is determined by electric conductivity.

本文采用电导率测量的方法实验研究了空气—正癸烷—亚硫酸钠和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷、1-十二碳烯和十三醛混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—三间磺化苯基膦三钠盐和十六烷基三甲基溴化铵水溶液四个三相体系的液液分散特性。当有机相与水相体积比分别为7/3、1/1和3/7时,前三个体系均形成水包油型分散,并有稳定的乳状液生成。

In this dissertation, two series of rare earth initiators: rare earth tri (2, 6-di-tert-butyl4-methylphenolate) and rare earth calixarene complex have been developed to ring-opening polymerize 2, 2-dimethyltrimethylene carbonate, trimethylene carbonate and ε-caprolactone at mild conditions. Both the polymerization features and mechanisms are discussed in details.

本论文为环碳酸酯、内酯的开环聚合开发了两类新型低毒性稀土催化剂:三(2,6-二叔丁基-4-甲基苯氧基)稀土配合物和对叔丁基杯芳烃稀土配合物,都可以在温和的反应条件下单组分引发2,2-二甲基三亚甲基环碳酸酯、三亚甲基环碳酸酯和ε-己内酯聚合,合成各种分子量的均聚和共聚材料,考察了催化剂结构、聚合条件等对聚合的影响,深入研究了聚合机理。

Their half lives were 27.29 and 69.16 days respectively. Monocrotophos degraded into both methanol extractable and unextractable residues in the submerged soil.The analysis of 14C-methanol extractable. residue by TLC and ARG indicated that degraded products of monocrotophos were triether phosphate,;N-de-methyl Azodrin, O, O-dimethyl-vinyl-phosphatc,O-demethyl Azodrin,and O, C-demethyl Azodrin.The principal degraded extratable product of monocrotophos was triether phosphate, which incresed from 5% of the parent solution of monocrotophos to a range of 28-83%, furthermore and its degradation process was much faster in the unterilized soil than in the sterilized soil.

久效磷在淹水土壤中降解为甲醇可抽提态和不可抽提态残留物,对甲醇可抽提态残留物经TLC和红外光谱分析表明,其降解物为磷酸三酯、N-去甲基久效磷、O,O-二甲基乙烯基磷酸酯,0-去甲基久效磷,以及O,C-去甲基久效磷,但主要为磷酸三酯,其含量由母液中的5%增加到28—83%,这种降解过程,在未灭菌土壤中比在灭菌土壤中快。

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

设计1组特殊荧光标记探针以构建1种检测p16基因启动子区3个CpG位点甲基化改变的芯片,特殊荧光标记探针包括1对非甲基化探针和甲基化探针,检测相邻的3个CpG位点甲基化的程度。

In solvent and under the action of certain catalyst, anilino methyl siloxane is reacted to obtain solid cage anilino methyl sesquisiloxane octamer acid salt; and through filtering, washing, precipitation and reaction with amine desalting agent, cage anilino methyl sesquisiloxane octamer is finally prepared.

本专利提供了一种笼型八聚苯胺甲基倍半硅氧烷的制备方法,苯胺甲基硅氧烷在一定溶剂和催化剂作用下反应,过滤得到笼型八聚苯胺甲基倍半硅氧烷酸式盐固体,经过洗涤、沉淀,再与胺类脱盐剂作用,最终制得笼型八聚苯胺甲基倍半硅氧烷。

Simultaneously we prepared benzimidazolines with two methods by the of reduction benzimidazolium salts with NaBH_4 and Grignard addition with benzimidazolium salts. Thus we provided a new biomimic synthetic method for 13 kind of tetrahydroisoquinoline starting from carboxylic aicd and halid, gave a new idea of the synthesis for new drugs.It can make many kind ofβ-carboline alkaloids which have bioactivity to antibacterium, antivirus and antitumor to start from 1,2,3,4-tetrahydro -β-carboline.

本文以1,3-二甲基苯并咪唑碘盐和2-取代的1,3-二甲基苯并咪唑碘盐为原料,采用了NaBH_4还原和Grignard试剂加成两种方法,制备了13种1,3-二甲基苯并咪唑烷和2-取代的1,3-二甲基苯并咪唑烷作为四氢叶酸辅酶一碳单元转移新模型,在酸性条件下,以2-(3,4-二甲氧基苯基)乙胺作为接收一碳转移的亲核试剂,完成了13种重要的四氢异喹啉类化合物的新合成方法研究。

On the discussion about mechanism of mutation of methylating, we explored thermodynamics and dynamic properties on base pairs, investigating the proton transfer on base pairs.

甲基化对嘌呤碱基电荷的影响,到甲基化引起碱基配对的改变造成碱基配对的诱变等都作了较为详细的探讨;在对甲基化诱变的探讨中,我们进行了热力学和动力学方面的研究,同时对碱基之间的质子迁移的难易程度也作了探讨;并在充分考虑水分子存在的情况下研究了甲基化对碱基之间的氢键作用和堆积作用的影响。

DNA methylating inhibitor makes the transfer of the methyl group to adenine or cytosine impossible,thus the reaction of DNA methylation is inhibited,the genomic methylation level is decreased.

DNA甲基化抑制剂使甲基基团不能转移到腺嘌呤或胞嘧啶,导致DNA甲基化反应受阻,从而使基因组甲基化水平降低。

Methods genomic dna was extracted from different stages of malignant transformation and tumorigenicity in inoculated athymic mouse 16hbe cells induced by cadmium chloride and methylating status of genome p16 gene promoter was observed with methylation-specific pcr and then compared with the controls.

从各组cdcl2恶性转化不同阶段及接种裸鼠成瘤的16hbe细胞中提取全基因组dna,采取甲基化特异性pcr法(methylation-specific pcr,msp)检测该基因组p16基因启动子区的甲基化状况,与非转化的16hbe对照细胞进行比较,并用去甲基化因子5-azac (5-aza-2'deoxycytidine)处理有异常甲基化的细胞。

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然而现在,所有的孩子都要在综合学校继续学习,所以这次考试只是决定他们将要学习哪些课程。

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