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The two components were measured by excitation-emission matrix fluospectroscopy both in simulated and real system. The results of measured data, which were resolved by alternating trilinear decomposition method . It was shown that it was feasible and simple method in the determination of two main components in the complex cortex fraxini without pretreatment and separation.

利用三维荧光进行测定,然后利用交替三线性分解算法对该数据进行分解,模拟样本和实际样本分辨和回归结果表明,在秦皮中其它荧光背景干扰共存下,可实现对秦皮萃取液中两种主要成分秦皮甲素和秦皮乙素的同时分辨和定量测定。

It included the development of the trilinear decomposition algorithms as well as the applications of these algorithms to different fields of analytical chemistry, in particular, to three-way fluospectroscopy (excitation-emission matrix fluospectroscopy)In Chapter 2, a method of simultaneous determination of two main components of the Chinese traditional medicine cortex fraxini was introduced.

第二章利用激发-发射荧光分析方法分析了中药秦皮粉末萃取液,结合交替三线性分解算法,在无须预分离的情况下,直接对秦皮实际萃取液中秦皮甲素和秦皮乙素这两种主要成分同时定性定量分析,方法允许在其他组分的干扰共存。

The influence of acidity on fluorescence and absorption spectra were investigated. Using quinine bisulphate as a reference, fluorescence quantum yield of aesculin in the condition of pH=4.96 was determined to be 0.76 at excitation wavelength 335nm, and in pH=9.40 was 0.80 at 375nm.

探讨了酸度与浓度对其荧光和吸光性质的影响,以硫酸奎宁为参比,测得秦皮甲素水溶液的两种强荧光型体的荧光量子产率,在pH=4.96,激发波长335nm条件下,荧光量子产率为0.76;在pH=9.40,激发波长375nm条件下,荧光量子产率为0.80。

According to the fluorescence differences of aesculin and aesculetin at different pH values, a fluorescence method was developed for the determination of aesculin and aesculetin in Cortex Fraxini.

利用秦皮甲素和秦皮乙素在不同pH条件下荧光性质的差异性,制定了秦皮药材中二者分别测定的荧光分析方法。

To 7. 0.However, the RA values for aesculin and aesculetin trended to level off with increasing pH at the range 7. 0-8. 5. The 〓 values of aesculin and aesculetin were obtained by means of the two-parameter non-linear regression.

根据不同pH值条件下获得的秦皮甲素和秦皮乙素的淌度,借助于二参数非线性拟和的方法,我们首次成功地求出了秦皮甲素和秦皮乙素的离解常数。

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