用过的

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

He changed his mind upon meeting her, deciding her tomboyish attitude would be perfect for the part

WRIGHT自己在花絮中也说，KK的neck是他见过的女星中最美的，他常常用不同的镜头和角度去诠释她完美的脖子。

In trimera model, irradiated LB373 cells and other recombinant vaccines could elicit cellular and humoral response, which suggests that this model could be applied for evaluating effects of all vaccines targeting to human in addition to various transgenic animals.

在Trimera模型中，用照射过的LB373细胞和各种基因疫苗免疫后能激发出mage-3抗原特异的细胞免疫应答和体液免疫应答，提示这种模型可以通用于基于人的各种疫苗免疫效果的评价，这将是各种转基因动物之外的另一个备选方案。

For example they have been hacked and had a proxy server installed, they have an open relay email server, they are running an incorrectly configured proxy server, or they are running an unpatched web server that can be used to send spam.

例如，这些计算机一般都是被黑客攻击过的，并且还安装了代理服务器，这样就等于黑客拥有了一个完全公开的电子邮件服务器，错误的配置代理服务器或者安装带有漏洞的Web服务器软件的服务器都有可能被用来发送垃圾邮件。

Fad consumption makes people throw away the useful thing unrestrictedly, and this kind consumption pattern of" throwing away promptly after using " and " out of date to throw away promptly " accelerate circulation of commodities, at the same time it must waste a large number of resources.

时尚消费就是让人们无限制地扔掉有用的东西，这种&用过即扔&和&过时即扔&的消费方式在加速商品流通的同时，必然造成大量的资源浪费，是加剧生态恶化的&隐形杀手&。

I am so called the forum that ascends body center cavalcade just joined stationmaster network, I adore successful elder very much people, I long to succeed but I also know oneself and these elder people difference, I arrive to had not used office software now even without the culture, knowledge that does not have major, can contact namely CMS\BBS program.

我所谓的跻身站长行列只是入住了站长网的论坛，我很崇拜成功的前辈们，我渴望成功但是我也知道自己与这些前辈们的差距，我没有文化、没有专业的知识甚至到现在没有用过办公软件，能够接触的也就是CMS\BBS程序。

It's funny though because I wrote it as a "scribble" of my thoughts after I talked to some friends earlier in the night who were trying to get more people to read their blogs…then I ran across this Dale Carnegie book and so I thought I'd throw something short together to help people who are looking to do that.

它的搞笑，但因为我写的，它作为一个& Scribble用&我的想法后，我跟几个朋友早在晚间，他们正试图让更多人阅读他们的博客…那麼，我遇到这种戴尔卡内基书，所以我想过自己扔东西短，共同来帮助人的人，都是这样做的。

And in The Little Mermaid, Ariel swam through clusters of fish whose schooling was simulated, seaweed that swayed autonomously, and bubbles that percolated with physics.

而在《小美人鱼》中，Ariel游过的那些鱼群，它们的聚合、分离都是模拟出来的，海草的摇动是自动的，而那些泡泡则是用了物理学规律从水中渗出的。

Itis also important to note that Hamlet is extremely melancholy and discontented with the state of affairs in Denmark and in his own family—indeed, in the world at large. He is extremely disappointed with his mother for marrying his uncle so quickly, and he repudiates Ophelia, a woman he once claimed to love, in the harshest terms.

需要注意的一点就是哈还是一个非常忧郁的角色，他对丹麦的事务，对他的家庭，对这个世界都极其不满，特别是母亲与叔父结合之快更加使哈沮丧，而且他还用最刻薄的语言批判自己曾爱过的Ophelia。

In order to satisfy the user cognitive demands,the simplification map are used to provide the whole route information which includes only simplification route and the landmarks on the way,and the map display contents are visualized depending on the placename"s ranking;the paper also studies the different simplify principles from traditional map synthesis,that is ,releases the veracities of route geometry elements such as length and angle for the sake that the relative user"s task cognitive informations are displayed with clarity and legible on the limited screen;researches on the route simplify algorithms that preserving the topology-coherence.the paper exploits the application software for LBS navigation service.

在满足用户当前认知需求前提下，提出用简化路线图提供整个路线信息，简化图中包含已简化过的路线和路线两边的地标，而且地图内容可视化依据于地名等级；研究不同于传统制图综合原则的路线认知简化原则，即放松路线中几何元素的准确度，以确保与用户任务相关的道路认知信息最大清晰度地展现在有限屏幕上；研究保持拓扑一致性的快速路线简化算法。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。