用溴处理

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

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At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。