用显微镜

The components of products were analyzed by FTIR and the structure and morphology were characterized by X-ray diffraction and field emission scanning electron microscopy, respectively. The electrochemical performances were investigated by cyclic voltammetry and constant current charge/discharge techniques.

用红外光谱对所制样品的组分进行分析，用X射线衍射和场发射扫描电子显微镜表征产物的结构和形貌，用循环伏安和恒电流充放电等测试方法对其电化学性能进行研究。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中，我们应用免疫组织化学的双标方法，用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞，用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白，分别应用FITC和Cy3标记的荧光二抗来显示，最后用激光扫描共聚焦显微镜进行观察并定量分析。

Beijing 100034,China(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)Abstract : Objective In order to study the effects of ATP on U937 cells. Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used.

应用ATP(0.23g/L)作用于人白血病细胞U937，(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪文献检测 ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜文献检测凋亡小体的形成过程；(4)用苯胺黑染色文献检测凋亡小体的生命本性。

Then size properties and crystal characteristics of lily starch granules were observed with combination of photomicroscope, scanning electronic microscope , polaried light microscope and X-ray diffraction. Compared with potato starch and cornstarch, solubility, swelling power and freeze-thawing properties of lily starch were measured by centrifugal method; its thermal, rheological and mechanical properties were systematically investigated by DSC, Rheometer and Texture Analyser, respectively.

然后用光镜、电镜、偏光显微镜与X-衍射相结合研究了百合淀粉粒的表面结构和晶体结构特征，并与马铃薯淀粉、玉米淀粉相比较，用离心法考察了百合淀粉的溶解度、膨润力与冻融性能，用差示扫描量热仪、动态流变仪与质构仪等手段深入系统地研究了其热学特性、回生过程中的流变学特性和力学性能。

We observed the changes of the proprioceptors in the partly injuried ACL and the xenogeneic graft/artificial graft being used to reconstructing the injuried ACL with gold chlorid staining method and laser copolymerization electron micrography method to observe the morphological changes of the neuromechanism in the ligament tissue,with RT-PCR method to evaluate the proportion and the regeneration of the nerve component and with HRP retrograde tracking technology and electrophysiologic study to judge the functional changes of the neuromechanism in the ligament tissue,respectively.

本研究分别制作兔ACL部分损伤动物模型、异种肌腱移植物重建损伤ACL动物模型和LARS人工韧带重建损伤ACL动物模型，分别于术后不同时间点对损伤ACL/移植物进行氯化金染色光学显微镜检查和PGP9.5免疫荧光染色后激光共聚焦电子显微镜检查观察韧带内神经结构形态学变化，用RT-PCR法检测韧带中GAP-43和PGP 9.5的mRNA表达情况以评价损伤ACL/移植物内神经成分比例及神经再生情况，通过HRP逆行追踪技术和电生理检查(包括体感诱发电位和腘绳肌肌电图)评价损伤ACL/移植物内神经结构的功能情况。

Their morphologies were characterized with scanning electron microscopy, transmission electron m...

通过扫描电子显微镜、透射电子显微镜的观察，以及对产物的拉曼光谱分析发现，用本方法制备得到的碳纳米管形态良好，石墨化程度高。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Lamblia were cultivated axenically with modified TYI-S-33 medium contained dihydroartemisinin (0.002 mg/L) for 12 h and then stained by rhodanmine phalloidin and paclitaxel (P22310). The cytoskeletons of the organisms were detected and analyzed by flow cytometry, and then observed by confocal microscopy.RESULTS: The cytoskeleton of trophozoites was markedly damaged after treatment with 0.002 mg/L dihydroartemisinin for 12 h.

将用含双氢青蒿素的改良TYI-S-33培养基培养后的蓝氏贾第鞭毛虫滋养体，用专一性结合f-肌动蛋白的荧光染料罗丹明－鬼笔环肽(rhodanmine phalloidin， RDP)标记微丝，结合微管的紫杉醇(Paclitaxel， Oregen Green 488， P22310)标记微管，用流式细胞术检测、分析，用激光共聚焦显微镜(confocal microscopy， CFM)观察滋养体微丝和微管的变化。

With slight modification this kind of polarizing microscope can be used as gem microscope.

只要稍加改动这种偏光显微镜便可以用做宝石显微镜。

An optical microscope of good quality can produce magnifications of several thousands of times, but in practice it is employed only for magnifications up to approximately 1000 times.

一个高质量的光学显微镜的放大率是几千倍，事实上，通常使用的放大率只有1000倍，而电子显微镜则用来观察需要更高放大率的物体。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。