用显微镜

Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2％TTC染色，用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比；取视交叉前平面梗死边缘区皮层组织约100mg，用酶消化法制作神经细胞悬液，用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡，以细胞平均的荧光强度代表细胞内钙浓度；冰上迅速分离视交叉后梗死边缘区皮层组织约100mg，于低温下制作脑组织匀浆，用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

MAIN OUTCOME MEASURES:Inverted microscope and transmission electron microscope were used to observe cell appearance.

主要观察指标：①用倒置显微镜和透射电子显微镜进行细胞形态学观察。

The company's main products: color detection instruments: Glossiness Miriam, Miriam color spectrophotometry, standard light source for color light box, transmissioní°Miriam, Miriam orange peel, fresh young tester; surface coating physical properties testing instruments: Coating thickness, wet film thickness, temperature tracker, surface humidity instrument, dewpoint meter, roughness instrument, electric spark pinhole leak detectors; Detect mechanical properties of surface coating equipment: zoned grid, and pencil hardness tester , impact tester, hardness testing sticks, Latvia Law / hydraulic adhesion tester, abrasion tester, cylinder / cone bending tester, cups sudden device, surface tension instruments, scratch pen, indentation hardness tester, etc.; paint coating equipment dedicated category: fineness board, drying time recorder, wet adhesion tester, paint ohmmeter, PH, the coating device, mixer, bead mill, mixer, spray gun, putty detector Miriam, white tester, iron cobalt colorimeter, moisture titrator; viscosity-type equipment: Viscosity Cup, density cup, bubble viscosity tube viscometer, infiltration cup rheometer, viscosity units conversion meter; laboratory standard consumables: Mitsubishi pencil tests, 3M adhesive tape test, RCA tape, rubber, the hiding power test paper for color tubes, microscopes dedicated LED ring light source, transfer ink knife and ink scraper, brush, Color Card, gray cards, zirconia beads, glass beads, pieces of cement mortar, the standard of fly ash, the standard viscosity fluid, stopwatch, chemical industry-standard compilation of manuals, paint paint testing manual; test standard Material: tin plate, steel plate, aluminum sheet, asbestos-cement pressure plate, Eterpan, glass, maple panels, MDF, copper film, polyester film, copper; measuring meter and optical instruments: digital calipers, micrometer, dial gauge a high scale, depth gauge, thickness gauge, diameter gauge, gauge block, magnifiers, microscopes, projectors, video measuring instrument; environmental test equipment: oven, constant temperature and humidity box, hot and cold circulating water bath, salt spray test chamber, high temperature resistance furnace, fluorescent UV aging chamber, xenon lamp aging chamber; electronics equipment: infrared thermometer, multimeter, clamp sheet, thermal imager, temperature and humidity meter; anti-static instruments: static tester, surface resistivity device, flat-panel monitor, anti-static footwear tester, wrist strap tester, body Tester; other types of instruments and equipment: torque tester, temperature and humidity recorder, push pull, the vibrometer, speed instrument, analytical balance, industrial Scale Units, dust particle counter, X-ray fluorescence coating thickness measurement and elemental composition analyzer, and so on.

公司主营产品：外观颜色检测仪器：光泽度仪、分光色差仪、标准光源对色灯箱、透射雾影仪、桔皮仪、鲜映性测试仪；表面涂层物理性能检测仪器：涂层测厚仪、湿膜测厚仪、炉温跟踪仪、表面湿度仪、露点仪、粗糙度仪、电火花针孔检漏仪；表面涂层机械性能检测仪器设备：划格器、铅笔硬度计、冲击试验仪、硬度试验棒、拉脱法/液压式附着力试验仪、耐磨耗试验机、圆柱/圆锥弯曲试验仪、杯突仪、表面张力仪、划痕笔、压痕硬度计等；涂料油漆类专用仪器设备：细度板、干燥时间记录仪、湿附着力耐擦洗试验仪、油漆电阻计、PH计、涂膜器、混合机、珠磨机、搅拌机、喷枪、腻子测定仪仪、白度仪、铁钴比色计、水份滴定仪；粘度类仪器设备：粘度杯、密度杯、气泡粘度管、粘度计、渗透杯、流变仪、粘度单位换算计；实验室标准消耗品：三菱测试铅笔、3M测试胶带、RCA纸带、橡皮条、遮盖力测试纸、对色灯管、显微镜专用LED环型光源、调墨刀、墨铲、狼毫刷、色卡、灰卡、锆珠、玻璃珠、水泥砂浆块、标准粉煤灰、标准粘度液、秒表、化学工业标准汇编手册、涂料油漆测试手册；测试级标准基材：马口铁板、钢板、铝板、石棉水泥加压板、埃特板、玻璃板、枫木面板、中纤板、紫铜片、聚酯膜、铜棒；量具量仪及光学仪器：数显卡尺、千分尺、千分表、高度尺、深度规、厚度规、内径规、量块、放大镜、显微镜、投影仪、影像测量仪；环境测试设备：烘箱、恒温恒湿箱、冷热循环水浴、盐雾试验箱、高温电阻炉、荧光紫外老化试验箱、氙灯老化试验箱；电子类仪器：红外测温仪、万用表、钳形表、热像仪、温湿度仪；防静电仪器：静电测试仪、表面电阻仪、平板监测仪、防静电鞋袜测试仪、手腕带测试仪、人体综合测试仪；其他类仪器设备：扭力测试仪、温湿度记录仪、推拉力计、测振仪、转速仪、分析天平、工业台秤、尘埃粒子计数器、 X-射线荧光涂层测厚和元素成分构成分析仪，等等。

TThe tapping panel dryness in Hevea brasiliensis associated with wounds in the roots was investigated by using light microscopy and transmission electron microscopy.

用光学显微镜和电子显微镜技术研究了与根伤口联系的橡胶树割面干涸病。

Briefly, coronal brain sections (100 mm)were cut with a vibratome, postfixed with 4% glutaraldehydein 0.1 mmol/L cacodylate buffer (pH 7.4) for 1 h, andthen with 1% osmium tetroxide in 0.1 mmol/L cacodylatebuffer for 2 h. After rinsing with distilled water, the brainsections were treated with 1% aqueous uranyl acetateovernight, dehydrated in an ascending series of ethanolsto 100%, followed by dry acetone, and then embedded inDurcupan ACM. Ultrathin sections (0.1 mm) were cut andstained with 3% lead citrate and subsequently examinedwith a Zeiss transmission electron microscope (Zeiss Inc.,Thornwood, NY, USA).

简单的说，先用切刀制成100um的冠状脑切片，然后用含有4%戊二醛在0.1 mmol/L的二甲砷酸缓冲液(PH7.4)中固定1小时后，放在含有1%四氧化锇的0.1 mmol/L的二甲砷酸缓冲液中2小时，接着用蒸馏水冲洗后，放在1%的aqueous uranyl acetate中过夜，接下来用梯度酒精(至100%)脱水，然后用丙酮干燥，再用Durcupan ACM包埋，切成0.1um的超薄切片后用3%的lead citrate染色，随后用透射电子显微镜观察。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

Sulfide layer was made on the surface of high speed steel (W6Mo5Cr4V2) by means of low temperature ion sulfurization. The tribological properties of the sulfide layer under dry sliding were investigated on an MM 200 friction and wear test rig. An atomic force microscope and a scanning electron microscope equipped with an energy dispersive spectrometer attachment were adopted to analyze the morphologies and compositions of the original and worn surfaces of the sulfide layer. The phase structure of the sulfide layer was determined by X ray diffraction.

采用低温离子渗硫工艺在 W6 Mo5 Cr4 V2高速钢表面制备了硫化物固体润滑层，在 MM- 2 0 0型摩擦磨损试验机上对渗硫层的摩擦学性能进行了干摩擦试验研究，用原子力显微镜及扫描电子显微镜和 X射线光电子能谱分析渗层表面、截面、磨面的形貌及成分，用 X射线衍射仪分析了渗硫层相结构。

The preparative technique of solid lubricant is cold-press sintering method.The micro-structure , friction surface, lubricant transfer film surface of base material was characterized by SEM; XRD was used to do phase analysis. The degrees of crystallinity of pure PTFE under the different conditions of water cooling, air cooling and furnace-varying cooling were made comparison by XRD. The thermal stability of pure PTFE and solid lubricant was analyzed by DSC-TG; The composition and valence state of elements in the surface of carbon fiber ,solid lubricant before and after friction test and lubricant transfer film were characterized by XPS; The frictional property of solid lubricant was tested by pin and disk test rig .

用SEM表征原料微观结构、摩擦表面及润滑转移膜表面形貌：用XRD进行原料物相分析，及比较水冷却、空气冷却、随炉冷却三种工艺条件下的纯聚四氟乙烯树脂的结晶度；用DSC-TG分析纯聚四氟乙烯树脂及固体润滑剂的热稳定性；用XPS表征碳纤维表面、固体润滑剂摩擦前后表面、润滑转移膜表面的元素组成、价态变化；用销盘式摩擦磨损试验机对固体润滑剂进行摩擦性能测试；采用专用的台架模拟试验机对固体润滑剂镶嵌轴承套与钢轴摩擦副间的摩擦磨损性能进行测定；用莱次偏光显微镜测试固体润滑剂的硬度；用材料试验机测试固体润滑剂的抗压强度等。

In this work, nanostructure samples of La1-xCaxMnO3（x=0.2、0.3、0.4、0.5）were prepared by hydrothermal-precipitation method . The structural characterization of the powder samples were done by X-ray diffraction. XRD patterns showed that all the samples ate single perovskite-type phase withoutother impurity or secondary phase. The shape and size of samples were performed on Scanning Electron Microscopy. SEM photographs investigated that temperature of hydrothermal effects the phase of samples and alkalinity of the reaction mixture by ΔTad-T curve measurement device at low magnetic fielded

本论文采用水热共沉淀退火法制备了La1-xCaxMnO3（x=0.2、0.3、0.4、0.5）系纳米结构，用X射线衍射仪确定产物为单相钙钛矿结构，无任何杂峰及第二相；用扫描电子显微镜与透射电子显微镜分析了产物的大小和形貌，发现水热温度对产物的形成起决定作用，随着温度的升高，产物由薄膜状向丝状转变，而溶液的碱度可控制产物的形貌和大小，当碱度为6.35mol/L，水热温度为240 C时，La0.6Ca0.4MnO3的主要形貌为纳米丝并有少量纳米颗粒，纳米丝平均直径为40nm；最后测量了粉末样品在低磁场（1.4T）下的磁热效应，发现水热共沉淀退火法制备样品的最大绝热温度明显高于球磨法制备样品的最大绝热温变。

Surface relief caused by the formation of lower bainite in an Fe-C-Si-Mn alloy has been investigated by STM. It was discovered that the subunit which is the lowest structural unit observed by TEM is actually made up of sub-subunits. The surface relief due to the formation of sub-subunit is tent-shaped, and of a non-IPS type, which indicates that the sub-subunit cannot be formed by shear. The height of relief is 60-140nm, and the maximum shape deformation is ca.

用扫描隧道显微镜对Fe-C-Si-Mn合金中的下贝氏体浮突进行了观察研究发现用透射显微镜观察到的最小结构单元——亚单元是由更小的超亚单元组成的超亚单元的浮突为&帐篷&型，而非不变平面应变型，表明它不是切变形成；超亚单元的浮突高度为60-140nm，最大形状变型量约为0.23。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。