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METHODS:Antisense "knock-down" approach was used to inhibit MDR1 gene expression in bovine nonpigmented ciliary epithelial cells.

用反义寡核苷酸阻抑牛眼睫状体非色素上皮细胞MDR1基因表达,在激光共聚焦显微镜下检测MDR1基因反

3PH was done in group M and T. Then the rats in B group and M group were injected normal saline via portal vein, and those in T group were injected with SHCs suspension. Four week later, the level of serous alanine aminotransferaseand glutamic oxalacetic transaminase were tested, and liver pathological changes observed. Frozen sections of T group were observed under fluorescent microscope.

将实验用同一近交系SD大鼠30只随机平均分成空白对照组、损伤造模组和SHCs移植组。B组腹腔注射生理盐水,M组、T组腹腔注射CCl4.24 h后戊巴比妥腹腔麻醉,消毒开腹,M、T组行2/3肝切,B、M组门静脉注入生理盐水,T组门静脉注入经膜荧光标记SHCs悬液。4周后,采血测血清谷丙转氨酶、谷草转氨酶,并取病理标本做冰冻切片荧光显微镜下观察及HE染色普通光镜下观察。

The animal model of rabbit bullous keratopathy was established by anterior chamber injection with 0.05% benzalkonium bromide. The corneal thickness and corneal endothelial cell density were measured with ultrasonic corneal pachymeter and non-contact specular microscope separately before the animal models was established.

二、以0.05%的新洁尔灭溶液前房注射制作兔眼大泡性角膜病变的动物模型,制模前分别用角膜测厚仪和角膜内皮显微镜测量角膜厚度和角膜内皮细胞数目。

Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.

将其植入恒河猴背阔肌的肌袋内,以未转染GFP的BMSC用同样的方法接种块状可吸收HA上,作为对照,术后6周取材,4%的中性多聚甲醛固定,塑料包埋,制作骨磨片PI染色在激光共聚焦显微镜下进行观测。

The dislocation configuration and other related details of pearlitic heat-resistant steel 12Cr1MoV were observed and analyzed by using transmission electron microscope.

用透射电子显微镜对12Cr1MoV珠光体耐热钢的位错组态和其他细节进行了观察和分析。

Researchers have validated the mass deposition process in small biomolecules and pharmic molecules induced by heavy ion implantation, and researches on modification of...

将来可先应用稳定性和放射性重离子束注入生物和药物分子,然后用X射线衍射、电子显微镜放射自显影和图像处理等技术对重离子注入质量沉积开展进一步的研究。

Through the methods of polarizing microscope and cathodoluminescence, the diagenesis of Late Carboniferous phylloid algal reefs in southern Guizhou is studied, which indicates diagenesis are mainly of bio-binding and baffling actions, micritization, cementation, compaction-pressure solution, fracture, recrystallization and dissolution in type.

东北大学资源与土木工程学院,沈阳110004通过偏光显微镜研究和阴极发光技术,对黔南晚石炭世叶状藻礁灰岩的成岩作用做了详细研究,认为礁灰岩主要成岩作用类型有:生物黏结障积作用、泥晶化作用、胶结作用、压实压溶作用、破裂作用、重结晶作用和溶解作用等。

The platelets were labeled by FITC injected by jugular artery. Adherence of platelets to endothelial cells in pial vessel was observed by fluorescent microscope. The expression of GPⅢa protein was identified by CD61 histochemistry.

荧光显微镜下观察活体软脑膜微血管中血小板对内皮细胞的粘附,用CD61抗体经免疫组化方法观察缺血再灌注沙土鼠脑组织血管内血小板和内皮细胞上GPⅢa糖蛋白的表达。

R-PE was isolated from marine red alga Polysiphonia urceolata,and STM was used to investigate its topo- graphy.

R-藻红蛋白从海洋红藻多管藻中分离获得,然后用扫描隧道显微镜对其结构进行了研究。

Scanning tunneling microscope was used to investigate the in vitro assembly of R-phycoerythrin from the marine red alga Polysiphonia urceolata. The results showed that R-PE molecules assembled together by disc-to-disc while absorbing on HOPG surface, which just looked like the rods in the phycobilisomes.

将海洋红藻R 藻红蛋白吸附到刚揭开的高定向石墨表面上,然后用扫描隧道显微镜在纳米尺度上进行直接观察,发现纯化的R 藻红蛋白在体外自然聚集时,能够&面对面&的聚集在一起,形成非常规则的类似藻胆体的杆状结构。

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