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In this thesis, rat-tails were used as experiment objects, there are 40 slices including transverse and longitudinal, HE-dyed and undyed. Excited by femtosecond laser, the changes of backward SHG and TPEF versus sample preparation, excitation wavelength, excitation power, penetration depth and so on were observed by two-photon laser scanning confocal microscope, effect factors of backward SHG and TPEF and differences of both were discussed and compared, and related comments were also presented.

以鼠尾组织作为实验对象,共40个切片,分为横向和纵向、HE染色和未染色四组,采用飞秒激光器作为激发光,用双光于激光扫描共焦显微镜(two-photon laser scanning confocal microscope, TPLSCM)观察和分析了样品在不同的制备方式、激发波长、激发功率、扫描深度等条件下的背向SHG和TPEF的变化曲线,讨论和比较了生物组织的背向SHG和TPEF的影响因素以及二者之间的异同,并尝试对实验现象做出了一定的解释。

Stained with 0.4% trypanblau for 4 min, then counted the stained cells and unstained cells.

用0.4%台盼蓝直接染色4 min,显微镜观察,计数被染色细胞和拒染细胞。

Methods: Fifty four teeth with 54 interacanal fractured instrument were endodontically treated using G-G bur with ultrasonic techinque under dental operating microsc. The unsuccess rates were calculated and analysed.

收集2006年11月~2007年12月间根管内折断器械共计54例(54个根管),在根管手术显微镜下用G-G钻结合超声技术处理,记录折断器械在根管中的分布以及根管中的位置,分析失败病例的原因。

The morphological changes of VEC in each group were observed by the inverted microscope, and the expression of ICAM-1 in each group was detected by immunocytochemistry.

通过倒置相差显微镜观察和比较,各组内皮细胞在形态上的变化,用免疫细胞化学染色方法检测ICAM-1在各组内皮细胞中的表达。

Digestive the well-grown cells with pancreatic enzyme(0.25%), count under the microscope, and make them into one-celled suspension, with a density of 1×105/ml.

将培养瓶中生长状况良好处于对数生长期的前脂肪细胞用0.25%的胰酶消化,显微镜下计数并制成密度为1×105个/ml的单细胞悬液备用。

The rhizobia in the root nodules of Alhagi sparsifolia were studied by transmission electron microscopy.

用透射电子显微镜研究骆驼刺根瘤中的根瘤菌。

The effect of thermal diffusion on electrical properies of SrTiO3 multi-function ceramics with coating ion was studied by application of the even design expermental method. Microstructure and element analysis were examined by SEM and Energy-Dispersive X-Ray analyss techenolegy. No new intergranuar phrase were oberved.The relationship amang electrical properties,diffusion conditions of the materials were discussed.

采用均匀设计液相涂覆热扩散方法,研究了涂覆离子,热扩散条件等对SrTiO3系陶瓷电性能的影响;用扫描电子显微镜作微观分析,X射线能谱仪作元素分析,证实钠离子的涂覆热扩散未在陶瓷晶粒之间产生新相,钠离子在晶粒内表面形成了一扩散层,讨论了扩散条件与电性能的内在联系。

Scanning tunneling microscopy has been used to study the adsorption of aspartic acid on the Cu(001) surface at room temperature.

用扫描隧道显微镜研究了室温下天冬氨酸在Cu(0 0 1)表面的吸附行为。

Dimensions and positions of holes for stage clips and attachable mechanical stage on microscope stage

显微镜。载物台装压簧和移动尺用孔的尺寸和位置

METHODS: Benzhydroxamic acid derivative E was artificially synthesized. The gastric cancer line 7901 was selected to serve as the target cell. The effect of medicine E on tumor cell activity was examined by cell culture technology in vitro. The change of morphology and the ultrastructure after treatment with medicine E was observed with the optic microscope and the electron microscope.

以胃癌细胞株7901为靶细胞,采用体外细胞培养技术评价苯异羟肟酸衍生物E对肿瘤细胞活性的影响,通过光学和电子显微镜对E药物作用后细胞形态学进行观察,用免疫组化和RT PCR方法检测细胞凋亡。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。