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MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标:倒置显微镜下观察细胞形态;于培养1,2,3周采用40 g/L多聚甲醛固定,常规组织切片,苏木精-伊红染色;在培养5,10,14 d用Hoechs33258荧光法定量测定细胞内DNA含量,BCA法测定蛋白质含量。

By means of FTIR and QSSPCD and OPTICAL MICROSCOPE during different thermal process, It is found that the diffusion of hydrogen may passivate the defects/boundaries and it is beneficial to the lifetime of mc-Si.

本文用傅立叶红外光谱仪和准稳态光电导衰减法及光学显微镜等测试方法,采用不同的热处理工艺条件,研究了在氢气氛下退火氢对体缺陷和晶界的钝化作用,有利于少子寿命的提高。

Methods:Preparing pastille serum of Rehmanniae pill of Six Ingredients with low,medium and high doses and using 10% pastille serum with different doses to cultivate OS-732 cells.

制备低、中、高剂量的六味地黄丸含药血清。10%低、中、高剂量的六味地黄丸含药血清培养OS-732细胞,显微镜下观察细胞生长情况;进行细胞计数和绘制生长曲线;用MTT法检测细胞增殖;进行细胞碱性磷酸酶活性测定。

In the second part, peroxidase in a single neutrophil cell and topography are imaged at the same time.

第二章用扫描电化学显微镜同时成像单个活细胞内的过氧化物酶活性和细胞形貌。

X-ray photoelectron spectroscopy and atomic-force microscopy were used to characterize the chemical composition and surfacial profile of the films.

用X射线光电子能谱和原子力显微镜分别分析了薄膜的化学组分和表面形貌。

Phycoerythrin was microinjected into the right parietal lobe of the rat cerebral cortex.

将荧光蛋白作为脑淋巴引流的示踪剂微量注射到大鼠大脑皮层内,用荧光显微镜观察其在大脑皮层和颈部淋巴结的分布。

Apply the metalloscope, the scanning electric mirrorand energy spectrum analysis instrument etc. material analysis way and MLD-10 dynamic load abrasion tester, JB-36 three use the impact tester etc. test equipment to analysis and test the wearable performance of selected material and welding quality of welding sample. So as to make certain the best welding material and welding crafts between the welding of pickax boot wearable material and pickax body material in making tamping pick adopting parted type structure.

应用金相显微镜、扫描电镜、能谱仪等材料分析手段以及MLD-10动载磨料磨损试验机、JB-36型三用冲击试验机等试验设备,对所选材料的耐磨性能和焊接试件的焊接质量进行分析测试,以确定分体式结构制造捣镐的工艺中最佳镐靴耐磨材料及其与镐体材料捍接过程中的最佳焊接材料和焊接工艺。

About 0.2 mL of foam was transferred with a 2 mL glass pipet into the well of a glass microscope slide (Fisher, 76 × 26 mm2, 1.4-1.6 mm thick, the well is 18 mm in diameter and 0.5 mm deep).

大约0.2mL的泡沫用一根2mL的玻璃吸量管转移进显微镜玻璃载片的阱中(Fisher, 76 × 26 mm2, 1.4-1.6 mm 厚,阱的直径 18 mm,深 0.5 mm)。

The change from crystal to non - crystal was confirmed by observation of sample structure by micro - polariscope combining with X - diffraction spectrum.

用偏光显微镜观测处理后淀粉颗料结构,结合X射线衍射曲线确认淀粉由多晶态向非晶态的变化。

Following characteristics of pollen germination and pollen tube growth were observed with fluorescence microscope.

用荧光显微镜对自交不亲和强度不同的梨品种丰水和菊水自花授粉及以丰水为母本的异花授粉后的花粉萌发及花粉管生长特性进行观察。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。