用显微镜

After 3 days treatment with RA, the cells began to show phenotypical changes, assuming a neuronal-like morphology with contemporaneous formation of dendritic-like structures.

在诱导的d1、3、7，用相差显微镜观察细胞形态的变化；用免疫细胞化学染色、RT—PCR等方法检测p27^Kip1、p21Cip1及其相关的cdk2、cyclinE的变化。

SiO 2/TiO 2 superhydrophilic coating on glazed tile substrate was prepared by solgel method,the surface chemical composition, contact angle of water and photographs of surface of the coating were studied by use of XPS,photomicroscopy and SEM.

用溶胶 -凝胶方法在釉面砖上制备了SiO2 /TiO2 超亲水涂层，用XPS、光学显微镜和SEM研究了涂层的表面化学组成、水润湿角和表面形貌。

After 2, 4, 6, and 8 weeks of feeding, 2% fluorescein sodium was dropped into rat conjunctival saccus and breakup time of tear film was observed by cobalt blue light scanning under slit lamp microscope. Then the rats were executed after anesthesia.

分别饲养2，4，6，8周后，用2%荧光素钠滴入大鼠结膜囊内，瞬目后在裂隙灯显微镜下用钴蓝光扫描照射观测大鼠的泪膜破裂时间，然后麻醉下颈椎脱臼法处死大鼠。

To collect RGCs photographs by Leica microscope with Z axis and CCD camera;to observe the changes of diameter, variance of structural features in dendritic field and somata after classification which according to the size and morphology.

方法用基因枪技术标记病程3个月的糖尿病大鼠和正常同龄大鼠的RGC，用带Z轴的Leica显微镜和CCD照相机采集RGC照片，按树突和胞体大小及形态分类后比较各组细胞树突野、胞体直径变化及其结构特征变异。

Methods Eighty patients of open heart surgeries performed recently were divided randomly into two groups, group A:heparin coated artery filter, group B:non-heparin coated. Platelets, hemoglobin, haematocrit, and activated coagulation time were measured on 60 minutes of extracorporeal circulation and after extracorporeal circulation and ultrastructure of filter screen was observed after extracorporeal circulation by electron microscope.

80例体外循环心脏直视手术患者随机分为两组，每组40例。A组：用肝素涂层动脉过滤器，B组：用非肝素涂层动脉过滤器；并于不同的时间点观察血小板计数、血红蛋白、红细胞压积、激活凝血时间和过滤器内网在电子显微镜下的改变。

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

The membrane formation processes include two main processes, the evaporation process was dynamically monitored with FTIR-ATR and the immersion process was dynamically photoed with optical microscope. The kinetic effect of the dry/wet phase inversion process on the morphology has been studied.

动态监测成膜过程的不同阶段，用傅立叶变换红外光谱仪来研究干相转化的蒸发过程，采用数码相机连续照相的方法，用光学显微镜来研究湿相转化的凝胶过程，并探讨成膜动力学过程对膜的最终结构及性能的影响。

Using polarizating microscope and reflecting microscope,we have studied the features of microstructure and basic composition of general silica brick and high quality silica brick used for large scale hot air stove and the effect of impurity content and high temperature reburning on microstructure.

用偏光和反光显微镜研究了普通硅砖与大型热风炉用优质硅砖的基本组成和显微结构特征，以及杂质含量和高温重烧对显微结构影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。