用显微镜

The resultant particles were characterized by X-ray diffractometer and scanning electron microscope.

用X射线衍射仪测定出产物的相结构，用扫描电子显微镜观察产物的微观结构。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌，接种在Dixon培养基32℃培养，分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光；分别用3％戊二醛溶液、pH4.0盐酸溶液、10％氢氧化钾溶液、1280μg／mL和64μg／mL氟康唑溶液、0.3％美蓝溶液处理后观察马拉色菌自发荧光的变化；选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

In theory, every problem of involving morphological analysis and examination can be solved with digital image processing technology. Because there are irregular form structures, uncertain space positions and impure samples of the human helminth eggs, digital application and research is underdeveloped in parasitology. The recognition and analysis of human helminth eggs is unable to be performed by means of automatic instrument as blood cell. As a result, they have been observed and distinguished under the microscope through the naked eye for a long time.

从理论上说，凡是牵涉到形态分析和检测的问题都可以用数字图像处理技术来解决，但由于人体寄生虫虫卵的不规则的形态结构、空间方位和标本杂质较多等原因，数字图像技术在寄生虫学上的应用和研究很少，寄生虫虫卵的识别无法象血细胞分析一样用自动化仪器进行，长期以来只能依赖人眼在显微镜下进行观察分辨。

They used a technique, intravital microscopy, which permits researchers to illuminate blood vessels using fluorescent signals, enabling them to "see" reductions in white cells on the vessel wall and subsequent lessening in inflammation.

他们用一种技术，活体显微镜，它允许研究者照亮血管用荧光信号，使他们能够"看到"减少白色细胞在血管壁上和随后减轻炎症。

Its morphologies and structure were characterized by scanning electron micrograph and X-ray diffraction, and its electrochemical properties were studied by charge-discharge test.

用扫描电子显微镜和X射线衍射研究了材料的表面形貌和晶体结构，用充放电循环实验对制备的锂离子电池性能进行了测试。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

This examination is performed by dropping oil on the periungual area and examining with an ophthalmoscope set at diopter 40, or with a dissecting microscope.

进行这项检查需在甲周点滴油，用屈光度为40的眼底镜，或用解剖显微镜进行检查。

The surface morphology and composition of the films were characterized by scanning electron microscope and X-ray photoelectron spectrometer.

用电子束蒸发的方法在Ta衬底上制备了LaB6薄膜，用电子显微镜、X射线光电子能谱仪对样品的表面状况、化学组分进行了分析。

The nitrogen content in terms of atomic percentage under different conditions was identified by X-ray photoelectron spectra.

同时用X射线光电子能谱确定了不同条件下薄膜中N原子的百分比，用原子力显微镜对薄膜的表面粗糙度及表面形貌进行了测量和表征。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。