用合成法合成

The method comprises: reacting semicarbazide and terephthalaldhydic acid under stirring, and monitoring the end point with silica gel thin layer chromatography to obtain terephthalaldhydic acid semicarbazone.

本发明以氨基脲为原料，在搅拌下与对醛基苯甲酸反应，用硅胶薄层层析法监测反应终点，合成氨基脲缩对醛基苯甲酸。

The modified CTAB can isolate high quality RNA from wild jujube seedlings. After exorcizing DNA from RNA by DNase I, the cDNA fragment synthesized with M-MLV reverse transcriptase was 100bp to 1500bp, satisfied to the requirement of cDNA-PCR.

采用改良CTAB法提取了酸枣幼苗总RNA，用DNaseⅠ去除RNA中的DNA后，选用M-MLV反转录酶合成了cDNA第一链，片段大小在100～1500bp之间，可以满足下一步cDNA分析试验的要求。

Fluorescing compounds were immobilized in PVC membrane and covalent binding to surface of glass, respectively. pH response characteristics were investigated under different conditions.

分别用PVC膜包埋法和玻璃表面共价键合法固定自行合成的荧光分子，测定了不同条件下的pH响应特性。

CrO-Y2O3catalysts were prepared by a deposition-precipitation method and tested by fluorinating 2-chloro-1,1,1-trifleuoroethane (HCFC-133a) to synthesize 1,1,1,2-tetrafluoroethane (HFC-134a).

用沉积－沉淀法制备了CrO-Y2O3催化剂，考察焙烧气氛及温度对1，1，1-三氟－2-氯乙烷(HCFC-133a)气相氟化合成1，1，1，2-四氟乙烷(HFC-134a)催化性能的影响。

The synthesized high ester valuePVSK was characterized and compared with high ester value PVSK sample from Japanese company.

用红外光谱法对合成的高酯化度PVSK进行了表征，并与日本生产的高酯化度PVSK进行了比较。

Eleven α-V-group cation-substituted toluenes (1) and acetophenones (2)were synthesized. The equilibrium acidities of these compounds were determined indimethyl sulfoxide solution by the "Overlapping Indicator Method" and the homolytic C-Hbond dissociation energies were derived from a thermodynamic cycle combining pKawith oxidation potential of carbanion.

合成了α-位有第V、Ⅵ主族元素杂正离子取代基的甲苯系列（1）和苯乙酮系列（2）共11个Ylide前体化合物，用重迭指示剂法首次测定了其在二甲亚矾溶剂中的酸性解离常数PKa，同时结合碳负离子氧化电位的测定，通过热力学循环估测了它们的气相均裂键能。

Methods: Encapsulate synthesized MG7 mimotope peptide into nanoemulsion using magnetic and ultrasonic technique. Dialyse method was used to sterilize, HPLC to determine encapsulation efficiency.

人工合成胃癌MG7-Ag的模拟表位多肽，用磁力超声法将其包封于纳米乳剂中，通过透析纯化，HPLC检测其包封效率。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

Methods AGOs polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen. Then AGO-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性AGO多肽，以马来酰胺活化匙孔血蓝蛋白(keyhole limpet hemocyanin， KLH)作为载体构建多肽免疫原，通过致敏大白兔，制备系列兔抗人AGO多克隆抗体，然后用亲和层析法纯化抗体。

Base on the proposed method, we also implement several algorithms of HLS verifiers.

利用本论文所提出的验证方式，我们也实现了几种不同的系统阶层的验证演算法用来验证高阶合成的设计结果，并从实验数据中，归纳出最佳的验证演算法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失