用合成法合成

Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis.

建立硫芥中毒淋巴细胞模型，分别用单细胞凝胶电泳法检测淋巴细胞DNA损伤；用镀铜镉还原法和一氧化氮合成酶试剂盒检测中毒后淋巴细胞培养上清液一氧化氮和一氧化氮合酶的变化；用流式细胞仪和琼脂糖凝胶电泳法检测细胞凋亡。

Methods: Cell proliferation was measured by crystal voilet staining assay. The activity of lactic acid dehydrogenase in the upper layer of culture solution was tested to examine the damage to the cell membrane.3H-TdR was used to study the effect of porphyrin nitrogen mustard on the synthesis of DNA.

用结晶紫染色法测定卟啉氮芥对人肝癌细胞SMMC7721的光动力抑制效应，用测定培养上清乳酸脱氢酶活性方法研究光动力作用对人肝癌细胞SMMC7721细胞膜的作用，用3H-TdR掺入法研究光动力作用对人肝癌细胞SMMC7721DNA合成的影响。

In addition, the preparation and package and store for standard reference materials of antioxidant BHT are studied.

本论文主要叙述了对工业品防老剂BHT的重结晶提纯和区域熔融提纯(自行设计和制造了区域熔融仪，申请了发明专利)来制定防老剂BHT标准物质的研究：通过实验确定了乙醇重结晶提纯工艺；利用自行设计区域熔融装置，拟定操作规程和技术条件；确定提纯样品的高效液相色谱法和差示扫描量热法的纯度检测条件和实验方法；用高效液相色谱检验BHT标准物质的均匀性、稳定性；分别用高效液相色谱法和差示扫描量热法测定这种标准物质的不确定度评定及两种不同原理检测的定值分析；最后确定标准物质BHT的纯度和合成标准不确定度：标准物质的制备、包装及贮存。

Pillared montmorillonite, as a representative of Sandwich like pillared interlayered clays ,caught many scientist"s attention not only because of its special structure of two-dimensional sheets but also its excellent activity of catalysis and adsorption. It is well documented that the method to prepare PILM, ion-exchanging, needed a long periods and was affected by many factors, and that the product had low thermal stability especially hydro-thermal stability which was the primary obstruction of its using in large-scale production. At the same time, PILM was well documented prepared with Na-montmorillonite, while there were a few documents about PILM prepared with Ca-montmorillonite. The large cost of remodel of Ca-montmorillonite to Na-montmorillonite was another obstacle of the application of PILM, while Ca-montmorillonite was widely distributed in nature, as a result, to prepare PILM with Ca-montmorillonite has practical significance. What"s more, PILM was mainly studied as a catalyst in oil refining process till now, but rarely documented in other catalysis reactions, especially in coal liquification filed which also needed high activity catalyst.

以柱撑蒙脱石为代表的具有"三明治"结构的柱撑粘土矿物因其特殊的二维孔道结构和优良的催化和吸附性能近年来成为国内外广泛关注和研究的热点，但是目前国内外大量报道的PILM制备大多采用离子交换法，生产周期很长，影响因素很多，并且产物热稳定性不够高，特别是水热稳定性较差，这是其不能在实际中大规模生产和应用的主要障碍，因此制备一种高稳定性的PILM意义非常；文献中大量报道的都是钠基蒙脱石合成的柱撑粘土矿物，传统的离子交换法必须将钙基蒙脱石钠化改型，其成本相当高，而自然界中大量存在的却是钙基蒙脱石，直接用钙基蒙脱石合成柱撑粘土矿物有很现实的意义，但目前这方面的报道却很少见；另外，迄今为止，PILM作为催化剂的研究领域主要在石油的炼制中应用，而在煤炭的液化中却鲜有报道，如果能在煤炭转化领域应用这一成果，其影响也将是相当大的。

Pillared montmorillonite, as a representative of Sandwich like pillared interlayered clays ,caught many scientist's attention not only because of its special structure of two-dimensional sheets but also its excellent activity of catalysis and adsorption. It is well documented that the method to prepare PILM, ion-exchanging, needed a long periods and was affected by many factors, and that the product had low thermal stability especially hydro-thermal stability which was the primary obstruction of its using in large-scale production. At the same time, PILM was well documented prepared with Na-montmorillonite, while there were a few documents about PILM prepared with Ca-montmorillonite. The large cost of remodel of Ca-montmorillonite to Na-montmorillonite was another obstacle of the application of PILM, while Ca-montmorillonite was widely distributed in nature, as a result, to prepare PILM with Ca-montmorillonite has practical significance. What's more, PILM was mainly studied as a catalyst in oil refinin g process till now, but rarely documented in other catalysis reactions, especially in coal liquification filed which also needed high activity catalyst.

以柱撑蒙脱石为代表的具有"三明治"结构的柱撑粘土矿物因其特殊的二维孔道结构和优良的催化和吸附性能近年来成为国内外广泛关注和研究的热点，但是目前国内外大量报道的PILM制备大多采用离子交换法，生产周期很长，影响因素很多，并且产物热稳定性不够高，特别是水热稳定性较差，这是其不能在实际中大规模生产和应用的主要障碍，因此制备一种高稳定性的PILM意义非常；文献中大量报道的都是钠基蒙脱石合成的柱撑粘土矿物，传统的离子交换法必须将钙基蒙脱石钠化改型，其成本相当高，而自然界中大量存在的却是钙基蒙脱石，直接用钙基蒙脱石合成柱撑粘土矿物有很现实的意义，但目前这方面的报道却很少见；另外，迄今为止，PILM作为催化剂的研究领域主要在石油的炼制中应用，而在煤炭的液化中却鲜有报道，如果能在煤炭转化领域应用这一成果，其影响也将是相当大的。

This introduces several basic manipulative techniques of preparative chemistry and quantitative volumetric analysis including preparation and characterization of a derivative, recrystallization, determination of equivalent weight by using titration and determination of melting points.

这里介绍了几个基本的合成技术和定量分析法，包括衍生物的合成及其结构表徵，再结晶，用滴定法确定等当量和熔点测定。

In the paper, the multidentate carboxylic acid ligands were used to combine with Cd, resulting in the three dimensional MOCPs single crystals—C12H16CdN2O8, with a low temperature hydrothermal synthesis method. The sample was characterized with IR spectra to affirm the organic groups in the frameworks, single-crystal X-ray diffraction to determine the crystal structure and composition of the frameworks, N2 adsorption isotherms at low temperature to measure the specific surface area and pore size distribution, and with TGA/DGA analysis to scale the thermal stability. Furthermore, the dissolution property and the photocatalytic activity of MOCPs under the ultraviolet ray condition were explored.

本文用低温水热合成法，用多齿羧酸配体异烟酸和Cd配位合成出[Cd(C6H4O2N)2(H2O)4]∞三维晶体并进行了表征：采用红外光谱法确认骨架中的有机官能团；用单晶X射线衍射分析法测定MOCPs的晶体结构，并确定其骨架的组成；通过低温氮气吸附等温线测定了其比表面积和孔径分布；以热重分析和差热分析测定材料骨架的热稳定性；测定了晶体的溶解性能；最后以亚甲基蓝为目标降解物，对[Cd(C6H4O2N)2(H2O)4]∞的光催化活性及亚甲基蓝的氧化动力学进行了探索(来源：A2444BC论文网www.abclunwen.com)。

Pillared montmorillonite, as a representative of Sandwich like pillared interlayered clays ,caught many scientist"s attention not only because of its special structure of two-dimensional sheets but also its excellent activity of catalysis and adsorption. It is well documented that the method to prepare PILM, ion-exchanging, needed a long periods and was affected by many factors, and that the product had low thermal stability especially hydro-thermal stability which was the primary obstruction of its using in large-scale production. At the same time, PILM was well documented prepared with Na-montmorillonite, while there were a few documents about PILM prepared with Ca-montmorillonite. The large cost of remodel of Ca-montmorillonite to Na-montmorillonite was another obstacle of the application of PILM, while Ca-montmorillonite was widely distributed in nature, as a result, to prepare PILM with Ca-montmorillonite has practical significance. What"s more, PILM was mainly studied as a catalyst in oil refining process till now, but rarely documented in other catalysis reactions, especially in coal liquification filed which also needed high activity catalyst.

以柱撑蒙脱石为代表的具有&三明治&结构的柱撑粘土矿物因其特殊的二维孔道结构和优良的催化和吸附性能近年来成为国内外广泛关注和研究的热点，但是目前国内外大量报道的PILM制备大多采用离子交换法，生产周期很长，影响因素很多，并且产物热稳定性不够高，特别是水热稳定性较差，这是其不能在实际中大规模生产和应用的主要障碍，因此制备一种高稳定性的PILM意义非常；文献中大量报道的都是钠基蒙脱石合成的柱撑粘土矿物，传统的离子交换法必须将钙基蒙脱石钠化改型，其成本相当高，而自然界中大量存在的却是钙基蒙脱石，直接用钙基蒙脱石合成柱撑粘土矿物有很现实的意义，但目前这方面的报道却很少见；另外，迄今为止，PILM作为催化剂的研究领域主要在石油的炼制中应用，而在煤炭的液化中却鲜有报道，如果能在煤炭转化领域应用这一成果，其影响也将是相当大的。

Pillared montmorillonite, as a representative of Sandwich like pillared interlayered clays ,caught many scientist's attention not only because of its special structure of two-dimensional sheets but also its excellent activity of catalysis and adsorption. It is well documented that the method to prepare PILM, ion-exchanging, needed a long periods and was affected by many factors, and that the product had low thermal stability especially hydro-thermal stability which was the primary obstruction of its using in large-scale production. At the same time, PILM was well documented prepared with Na-montmorillonite, while there were a few documents about PILM prepared with Ca-montmorillonite. The large cost of remodel of Ca-montmorillonite to Na-montmorillonite was another obstacle of the application of PILM, while Ca-montmorillonite was widely distributed in nature, as a result, to prepare PILM with Ca-montmorillonite has practical significance. What's more, PILM was mainly studied as a catalyst in oil refinin g process till now, but rarely documented in other catalysis reactions, especially in coal liquification filed which also needed high activity catalyst.

以柱撑蒙脱石为代表的具有&三明治&结构的柱撑粘土矿物因其特殊的二维孔道结构和优良的催化和吸附性能近年来成为国内外广泛关注和研究的热点，但是目前国内外大量报道的PILM制备大多采用离子交换法，生产周期很长，影响因素很多，并且产物热稳定性不够高，特别是水热稳定性较差，这是其不能在实际中大规模生产和应用的主要障碍，因此制备一种高稳定性的PILM意义非常；文献中大量报道的都是钠基蒙脱石合成的柱撑粘土矿物，传统的离子交换法必须将钙基蒙脱石钠化改型，其成本相当高，而自然界中大量存在的却是钙基蒙脱石，直接用钙基蒙脱石合成柱撑粘土矿物有很现实的意义，但目前这方面的报道却很少见；另外，迄今为止，PILM作为催化剂的研究领域主要在石油的炼制中应用，而在煤炭的液化中却鲜有报道，如果能在煤炭转化领域应用这一成果，其影响也将是相当大的。

First, this paper reports measurements of surface tension of ionic liquid EMIES (1-ethyl-3-methylimidazolium ethyl sulfate) using the forced bubble method at 278.15 to 323.15 K and densities of EMIES using Westphal balance at 278.15 to

为此，本文以廉价工业原料合成的硫酸乙酯—1—甲基—3—乙基咪唑EMIES和对水、空气都稳定的离子液体BMIBF_4为主要对象，使用密度法和最大气泡法等实验方法对这两种离子液体及其EMIES的水溶液的性质作了如下研究工作：在278.15—338.15K范围内用Westphal天平测定了EMIES和BMIBF_4的密度，用最大气泡法测定了纯离子液体EMIES和BMIB_4的表面张力。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失