用光

Use biochemical method to observe the effect of electro-acupuncture on AchE、SOD、MAO、NO、NOS of senile dementia AD rat's brain tissue; use immunohistochemistry technique to observe protein kinase expression of hippocampal; use optical and electron microscope to observe morphosis change of rat's brain tissue; use in situ hybridism technique to observe aspartate receptor mRNA expression of hippocampal; use water and electric maze experiment to observe rat escape latent period and mistake times, to understand it's learning and memory ability.

分别用生化方法观察电针对AD大鼠脑组织胆碱酯酶、超氧化物歧化酶、单胺氧化酶、一氧化氮、一氧化氮合酶的影响；用免疫组化方法观察海马组织蛋白激酶的表达；用光、电镜方法观察大鼠脑组织形态结构的变化；用原位杂交方法观察海马组织天门冬氨酸受体rnRNA的表达；用水迷宫和电迷宫实验观察大鼠逃避潜伏期和错误次数，以了解其学习记忆能力。

In automatic acquisition and procession of the photoelastic data, there are some troubles about how to acquire single isoclinics and to improve the precision of evaluating isochromatic fringe orders in the thin slice of the three-dimension model when the actual problems are solved by photoelasticity.

中文题名彩色域数字光弹性中数据采集技术的应用研究副题名外文题名 Data acquisition techniques in color-field digital photoelasticity 论文作者雷振坤导师云大真学科专业固体力学研究领域\研究方向学位级别博士学位授予单位大连理工大学学位授予日期2002 论文页码总数168页关键词光弹性法图像处理数据采集彩色图像处理数字光弹性馆藏号BSLW /2003 /O348 /5 用光弹性实验处理实际问题时，在数据自动采集与处理中，如何获得单纯等倾线的问题仍是一大难点，此外就是如何进一步提高三维冻结模型薄切片的等色线定级精度问题也比较困难。

The main principle of this technique is to control sensitization time of photographic plate by light impulse instead of shutter.

高速频闪摄影技术的主要特点在于：用光脉冲代替相机快门，以控制底片的感光；在光脉冲时间非常短(本文最小光脉冲宽度可达8μs，其技术指标代表目前国内的最高水平)的情况下，达到对高速运动的流体"冻结"而拍照、观察的目的。

Optical time-domain reflectometry located the regions where optical attenuation occurred due to leakage; optical power meter acquired the transmitted power at the end of fiber, and these events could be identified according to the characteristics of signals.

在光入射端，用光时域反射计对上述事件引发的损耗进行定位；在光输出端，用光功率计检测光功率的变化，并根据信号特徵来判定事件的类型。

Second, in this paper we recite and analyse how the painting masters of the different history stage used light and colors from "Prehistorical art" to "Rome art" imitating light and colors,"Middle ages art" creating "Sanctitude light","Revival of learning" representing nature light and colors and then light was used to create the drama light, Impressionistic using the light of science and impairing light instead emphasizing sensibility colors in modern times. Painting lives by the light and colors all along in the painting history.

从史前艺术、希腊罗马艺术的模仿光与色，到中世纪艺术创造神圣之光，再到文艺复兴的再现自然光线与色彩，然后到17世纪的主观处理光线形成"戏剧之光"、印象派的科学用光、现代绘画淡化光线并强调情感色彩，光线和色彩一直贯穿在历史的绘画长河里。

Second, in this paper we recite and analyse how the painting masters of the different history stage used light and colors from "Prehistorical art" to "Rome art" imitating light and colors,"Middle ages art" creating "Sanctitude light","Revival of learning" representing nature light and colors and then light was used to create the drama light, Impressionistic using the light of science and impairing light instead emphasizing sensibility colors in modern times. Painting lives by the light and colors all along in the painting history.

从史前艺术、希腊罗马艺术的模仿光与色，到中世纪艺术创造神圣之光，再到文艺复兴的再现自然光线与色彩，然后到17世纪的主观处理光线形成&戏剧之光&、印象派的科学用光、现代绘画淡化光线并强调情感色彩，光线和色彩一直贯穿在历史的绘画长河里。

New reactions found in this program include: photoinduced regioselective free radical addition to aromatic imines;photoinduced Dielse-Alder reactions of aromatic imines for synthesis of tetrahydroquinoline derivatives;Photoinduced radical cation mediated selective ring opening reaction of epoxides;Formation of novel macrocyclic crown ethers by photoinduced reaction of diazonaphthaquinones;photoinduced selective desilylation of silyl ethers.

用这种方法成功的发现了下列在有机合成中有意义的新反应：(1)光诱导产生的自由基正离子诱导的自由基对芳香亚胺的区域选择性加成反应；(2)用光诱导的芳香亚胺的Diels-Alder反应合成四氢喹啉衍生物；(3)经光诱导产生的自由基正离子基诱导的环氧化物选择性开环反应；（4）重氮萘酮在环醚中经光诱导反应形成新奇的大环冠醚；（5）硅醚的光诱导选择性脱硅基反应。

Obi-Wan and Anakin, both with blue sabers, fight on Mustafar as lava erupts around them.

Ep3欧比王跟安纳金在Mustafar的火山熔岩之前用光剑对决，两个人用的都是蓝色的光剑

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

It can be used in Portraiture,Ad photograph,movie ＆ TV.you mustnot take your chance by estimate when you deal with model of the 1ight and shadow.the colour temperature 4800—5200K,controls and deploys,Shoots strong air blower heat dissipation.the time of exposure can be freely controled assign light freely,photograph addio—vi sual,low cost long life.fan with strong power radiate.

人像摄影用光、广告摄影用光、电影影视摄影用光，拍摄时处理光影造型，再也无须凭估计碰运气。色温4800—5200K，曝光时间自由控制，随意布光拍摄直观，低成本长寿命，强力风机散热。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。