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Methods The method of GOD with 2,4,6-tribromo-3-hydroxybenzoic acid as a new chromogen was employed to make paper strips.

方法用2,4,6-三溴-3-羟基苯甲酸为色原的葡萄糖氧化酶法试剂制作试纸条,用CCD检测反应后的唾液葡萄糖检测试纸条的色度。

American Irish likes to wear 3 leaves clove, the ensign color that uses Ireland -- room of green yellow dichromatic adornment, wear green dress, give decorations of 3 leaves clove to wait to guest.

美国的爱尔兰人喜欢佩带三叶苜蓿,用爱尔兰的国旗颜色——绿黄两色装饰房间,身穿绿色衣服,并向宾客赠予三叶苜蓿饰物等。

Methods: The sample was absorbed with glass fiber filters paper and was desorpted by toluol. Trinitrotoluene was analyzed by capillary gas chromatography method with ECD detector.

用玻璃纤维滤纸采集空气中的三硝基甲苯,用溶剂洗脱后,经色谱柱分离,电子捕获检测器检测。

The detectable group, biotin-ε-aminocaproic acid N-hydroxysuccinimide ester, is attached to the remaining amino group. A series of multiamine compouds, i. e. ethylendiamine, 1, 6-hexandediamine, triethylenetetramine, tetraethylenepentamine, and synthetic polyarylamide are used inlabelling, it is found that linker arms and steric hindrance effect the detection sensitivity. The probes labeled by 1, 6-hexandediamine and triethylenetetramine can detect 0.1-0.5pg target DNA in dot blot hybridization, and their sensitivities are better than the others.

本文详细考察了乙二胺、己二胺、二乙烯三胺、三乙烯四胺、四乙烯五胺及合成的聚乙烯胺化合物对λDNA探针的标记和检测的影响,发现胺化物链长和空间位阻影响探针的杂交和检测,用己二胺和三乙烯四胺标记的生物素探针在杂交24小时、显色8小时后检测灵敏度达0.1-0.5pg,超过其它胺化物标记的探针。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Omeprazole racemate was resolved respectively by simulated moving bed chromatography and batch chromatography with the coated cellulose triphenylcarbamate as chiral stationary phase and ethanol containing 0.1% diethylamine as mobile phase.

用纤维素三苯基氨基甲酸酯涂敷型手性固定相和含0.1%二乙胺的乙醇溶液,研究了模拟移动床色谱和间歇色谱对奥美拉唑对映体的拆分。

Moreover,it was demonstrated that the threesubstances,E,DE and DA,increased dramatically and quickly after LPS treatment.Also,it was revealed that the changes of three substances were more quickly than thechanges of the above mentioned APPs during the same LPS treatment.Thesesuggested that the three substances existed as neurotransmitter.The presence and thechange of the CAs in amphioxus after treated with LPS indicated the evolutionaryexistence of CAs and acute phase response in the protochordate lineage.

第四,我们用高效液相色谱法(High Performance Liquid Chromatography,HPLC),探讨了LPS感染文昌鱼儿茶酚胺类神经递质的变化,优化了同时测定文昌鱼体液中NE、E和DA三种CAs的HPLC方法,并首次发现,文昌鱼体液中同时存在NE、E、和DA三种CAs物质,其中,E在文昌鱼中首次报道,这三种物质在LPS感染文昌鱼后浓度升高非常明显,与同期的APPs相比变化更为迅速,也体现了神经递质的主导作用,文昌鱼的CAs系统也具有了类似脊椎动物CAs系统的功能,可以初步判断CAs系统的进化源头由脊椎动物可推回到头索类。

This paper studied four pretreatment methods by adding three different retention time organochlorine pesticides(α-BHC、Dieldrin、pp′-DDT) to fish sample and making different concentration(0.01、0.1、1),then using GC-ECD determinate its recoveries.

研究了用气相色谱——电子捕俘器法测定鱼肉中有机氯农药的四种不同前处理方法。通过向鱼样中加入三种不同保留时间的有机氯农药(α—六氯苯、狄氏剂、滴滴涕),并测定三种不同浓度(0.01、0.1、1)时加标回收率。

The Irish people like to wear shamrocks, decorate rooms with the colors of Ireland's flag—green and yellow, and dress in green, presenting shamrock-themed treats to guests.

美国的爱尔兰人喜欢佩带三叶苜蓿,用爱尔兰的国旗颜色——绿黄两色装饰房间,身穿绿色衣服,并向宾客赠送三叶苜蓿饰物等。

We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取:收集新疆不同地区分离得到的利什曼原虫前鞭毛体,经培养,用0.01mol/L pH7.2的磷酸盐缓冲液漂洗,超声粉碎,比色法测定其蛋白质含量,间接ELISA法滴定出抗原的工作稀释度。2、检测:用提取的三株抗原,分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体,并以肺结核病人血清、非疫区健康人血清做对照,三株抗原所用检测方法一致,血清工作稀释度均为1∶100。

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My first article for Screen Power Magazine, which is to this day is the official magazine of Jackie Chan, was a DVD review of the Hong Kong actioner Gen-X Cops, starring Nicholas Tse and Daniel Wu.

我的第一篇文章为屏幕力量杂志,在对这天是Jackie Chan 正式杂志,是香港actioner Gen X 警察的DVD 回顾,担任主角Nicholas Tse 和丹尼尔吴。

His lips grazed hirs, Now what are you going to do?

他的唇使吃草了 hirs,现在你将要做什麼?

I wasn't a huge drinker, but I enjoy it socially.

我不是一个贪杯的人,但在社交场合我会略微享受一点。