用

It is that:(1) the related compounds in Metronidazole Buccal Tablets prepared by Chongqing Medical College can be tested by determining (321nm) the total peak area of the impurities;(2) The related compounds in Metronidazole Disodium Phosphate for Injection prepared by Chongqing Medical College can be test by determining (254nm) the total peak area of the impurities and the peak area of the largest impurity;(3) Ceftriaxone polymer in Ceftriaxone Sodium for Injection prepared by Chongqing Keru Pharmacy Co.

结果如下：①用HPLC一UV法，在321nm处检测，用归一化法计算，控制各杂质峰面积的和，可检查重庆医科大学的注射用甲硝吟磷酸二钠中的有关物质：②用HPLC一UV法，在254nm处检测，控制杂质峰面积的和及控制最大杂质峰面积，可对重庆科瑞制药股份有限公司的注射用头饱曲松钠中的有关物质实施控制；③用HPLC一Uv法，在254nm处检测，按外标法计算，可检查注射用头抱曲松钠中的聚合物；④用紫外分光光度法，在277nm波长处测定吸收度，按C6H9N303的吸收系数为377计算每片标示百分含量，可测定重庆医科大学的甲硝哇口颊片的含量均匀度；⑤用紫外分光光度法，在277run波长处分别测定释放溶液20min、50min和90min的吸收度，按甲硝哇(C6H剥303)的吸收系数为377计算释放度，可检查重庆医科大学的甲硝哇口颊片的释放度。

On the base of the advanced theories, research methods from home and abroad, intensive use of urban land on the connotation and extension have been defined, also the driving factors of intensive use land in Xinjiang have been analyzed. As the case of nineteen cities of the Xinjiang Uygur Autonomous Region, from the land input level, the extent of land use, land-use efficiency and land use in four areas of eco-environmental quality, the establishment of a level of intensive use of urban land evaluation system has been done in this ***. Using the methods of Principal component analysis, Spearman rank correlation coefficient and ArcGIS, calculate the principal city of the intensive use of urban land points between 2001-2006, the municipalities of urban Land Use Composite Index, build the three-dimensional display model of Intensive use level of urban land, then analyses the characteristics of spatial-temporal variance of the urban land intensively utilization. Finally, analysis propose measures and advices.

本文反在呼取海外外相闭实际和研讨方式的基本上，闭于城市土地集约当用的外涵及外延入行了界订，并闭于旧疆土地集约当用的驱动果荤入行了剖析，然后以旧疆19个从要城市为研讨区域，自土地投入火平、土地当用火平、土地当用效力和土地当用生态环境量量四个方里，树立了城市土地集约当用火平评价指本体解，采取从败开剖析法闭于旧疆2001—2006暮年城市土地集约当用火平入行了评价，盘算各从要市的城市土地集约当用得开，用秩相闭解数闭于所盘算出的各市的城市土地集约当用综开得开入行时光特征剖析；还帮ArcGIS软件，剖析旧疆城市土地集约当用火平的空间格局；自城市非工业己口、投入、产出取用地删加弹性三个方里剖析城市土地分体集约当用收铺趋背；最初降出了土地集约当用的闭于策建议。

Again she struggled with it, fighting him with her small, fine eyes, with the plush arrogance of a top dog, with her nascent transference to another man, with the accumulated resentment of years; she fought him with her money and her faith that her sister disliked him and was behind her now; with the thought of the new enemies he was making with his bitterness, with her quick guile against his wine-ing and dine-ing slowness, her health and beauty against his physical deterioration, her unscrupulousness against his moralities—for this inner battle she used even her weaknesses— fighting bravely and courageously with the old cans and crockery and bottles, empty receptacles of her expiated sins, outrages, mistakes.

她再次对他的才智进行反抗，用她细巧秀丽的眼睛，用一个优胜者极度的傲慢，用她刚萌生的移情别恋，用积累多年的怨愤同他较量；用她的金钱以及她相信她姐姐不喜欢他而支持她，用他因刻薄而招来新的对头这些想法同他较量；用她机敏的手段来对付他慢吞吞的饮酒吃饭；用她的健康和美丽来对付他身体的衰老；用她的肆无忌惮来对付他的道德信条——在这场内心的战斗中，她不惜以她的弱点为武器——犹如用破旧的瓶瓶罐罐，她用她已经受到惩罚的罪过、劣迹和错误来做勇敢无畏的抗击。

Used with caution in pregnant women. 2, estrogen medication during early pregnancy can cause fetal hydrocephalus, cerebral meningocele, visceral malformations, feminization of male fetus, testicular dysplasia, low reproductive capacity, pregnant women忌用. 3, pregnant women taking high-dose folic acid may be a high concentration of zinc metabolism in pregnant women do together which led to scattered and intrauterine fetal growth retardation and low birth infants rationale. 4, a cold fever of 39 degrees sustained for more than 24 hours can cause fetal facial malformations such as lip.

孕妇慎用。2、雌激素妊娠早期用药可致胎儿脑积水，脑脊膜膨出、内脏畸形，男胎女性化，睾丸发育不良，生殖能力低下，孕妇忌用。3、孕妇服用大剂量高浓度叶酸可干拢孕妇锌代谢而导致胎错落和宫内发育迟缓和低出生体理儿。4、感冒发热达39度以上持续24小时以上可引起胎儿面部畸形如兔唇。

Three companies inflatable station staff told reporters that the past few days with temperatures dropping, the public用气量a marked increase in filling Gas stations have to send gas 30%~ 50% increase in workers repeatedly unaspirated unaspirated不亦乐乎busy.

三家公司充气站的工作人员都向记者表示，这几天气温下降，市民的用气量明显上升，充气站的送气量都有30%~50%的增幅，送气工来来回回送气忙得不亦乐乎。

Trellised up 用棚架支撑着 in most tempting show 最诱人的样A fox had fallen into a well, and had been casting about for a long time how he should get out again, when at length a goat came to the place, and wanting to drink, asked Reynard whether the water was good, and of there was plenty of it .

&狐狸和山羊一只狐狸掉在一口井里，转了很久怎样再跳上去，最后一只山羊来到这里，他正想喝水，便问狐狸这水好不好，还多不多，狐狸掩饰起他的真实危险处境，回答说：&下来吧，我的朋友，这水好得使我喝不够，而且多的用不完。

From the analysis, we can conclude that at least three aspects will cause cross-cultural pragmatic failure: a. The intersection of pragmeme subset A and pragmeme subset B is an empty set; b. Interlocutor A's pattern of pract elements coupling is different from that of interlocutor B's; c. The outranging of allopract causes the transferring from a pragmeme to another pragmeme. The three aspects of pragmeme theory enrich the studies on cross-cultural pragmatic failure.

通过分析，我们得出跨文化语用失误的原因有三：第一，交际者双方对于某一语用行为的语用位交集为空集时会导致跨文化语用失误；第二，交际者双方选择的语用素集合中元素之间耦合方式不相同时会导致跨文化语用失误；第三，交际者一方的语用变体超出对方语用位范畴时会导致跨文化语用失误。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

To substitute…for :用…替代 You can substitute vegetable oil for butter in this recipe.

在这一食谱中，你可以用植物油代替黄油。

He melody of sth 用某曲调: We sang these lines to the tune of Yankee Doodle.

我们用扬基歌的曲调唱这几句歌词。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。