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Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化,以及对脱色、结晶方法的研究与摸索,最终确定强酸型阳离子树脂HZ-001在pH=3,3倍床体积流速,温度室温,对色氨酸有最大的吸附作用;将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱,再用2mol/L的氨水6倍床体积流速在室温下进行洗脱,收集洗脱液,60℃浓缩为过饱和状态后加入30%无水乙醇,4℃下100rpm摇起晶20h,过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

Commutative ion resin of strong acid instead of hydrochloric acid neutralized sodium hydroxide meliorated step of subsequent treatment.

用强酸型离子交换树脂代替盐酸中和氢氧化钠对产物的后处理步骤进行了改进。

The connection converting apparatus includes: a hook detector for detecting a hooked state of the telephone; a relay for connecting the telephone to either the Internet or the PSTN according to a commmand; a duplex circuit for suppressing voice leakage and side tone by converting and reconverting from a two-line signal of the telephone to a four-line signal for transmission and reception; a tone decoder for decoding a DTMF tone of the telephone; and a microprocessor for exchanging control data for the user's computer, controlling the relay to cause the telephone to be connected to the Internet when the hook detector detects an off-the-hook state of the telephone, and providing number data to the user's computer when the microprocessor receives number data from the tone decoder.

所述连接转换装置包括:用于检测所述电话的摘机状态的摘机检测器;用于根据命令将所述电话连接到互联网或PSTN的转接器;用于通过从所述电话的双路信号到用来传送和接收的四路信号之间的转换及复原来抑制声音泄漏和侧音的双工电路;用于对所述电话的DTMF音进行解码的音频解码器;以及微处理器,其用于交换来自所述用户的计算机的控制数据、控制所述转接器以使所述电话在所述摘机检测器检测到所述电话的摘机状态时连接到互联网、以及在所述微处理器接收到来自所述音频解码器的号码数据时向所述用户的计算机提供号码数据。

We hereby undertake to hold the said property on account o said bank and subject to its order for the purpose of ultimate sale or exchange or for the purpose of loading, unloading, storing, shipping, transshipping, manufacturing, processing, or otherwise dealing with the said property in a manner preliminary to sale or exchange or for the purpose of presentation, collection, renewal, or registration of transfer, and when sold to pay in the net proceeds to said bank.

我们在此保证为上述银行保存上述财产,并遵照其吩咐,最终出售、交换或在这之前装卸、库存,装运、转运、制造、加工、或用其他方式处理上述财产,或进行提示、托收,更换、或转让登记,如财产被出售,则将纯利交付给上述银行。

During the Great Proletarian Cultural Revolution, a "three-in-one" combination research group was organized from the workers, which formed the main-force, cadres and technical staffs of Silicen-Steel Sheet Works, Metallurgical Designing Institute and a research institute, for the recovery of sulphuric acid from the waste acid liquor of the steel works.

在无产阶级文化大革命中,由上海矽钢片厂、上海冶金设计院及中国科学院上海有机化学研究所组成的以工人为主体的工人、干部、科技人员三结合试验小组,对硅钢片酸洗废液通过用S-203强碱性阴离子交换膜扩散渗析法进行硫酸的回收,既节约物资,又改善环境,获得了较为满意的结果。

Three Texas drovers met on the highway and proceeded to dicker as follows: Says Hank to Jim:"I'll give you six pigs for a hoss; then you'll have twice as many critters in your drove as I will have in mine."

得克萨斯州的三个贩子在公路上碰头,打算进行下述的物物交换。汉克对吉姆说:&我用6 头猪换你1 匹马,那么你的牲口数将是我所有牲口数的2 倍。&

The adsorption effect was then verified by cation-exchange chromatography using 1-ml Streamline SP prepacked column and 28-ml Streamline SP self-assembly column.

用l mlSP预装柱及自装28 ml Streamline SP柱进行离子交换层析以验证吸附效果。

We hereby undertake to hold the said property on account of the said bank and subject to its order for the purpose of ultimate sale or exchange or for the purpose of loading, unloading, storing, shipping, transhipping, manufacturing, processing, or otherwise dealing with the said property in a manner preliminary to sale or exchange or for the purpose of presentation, collection, renewal, or registration of transfer, and when sold to pay in the net proceeds to the said bank.

我们在此保证为上述银行保存上述财产,并遵照其吩咐,最终出售、交换或在这之前装卸、库存、装运、转运、制造、加工,或用其他方式处理上述财产,或进行提示、托收、更换,或转让登记,如财产被出售,则将纯利交付给上述银行。

Methods Streptomycin and dihydrostreptomycin were extracted from honey samples using a liquid extraction with a phosphate buffer followed by a solid-phase extraction based on weak cation-exchange columns. After sample preparation, the hydrophilic interaction chromatography was used to retain streptomycin and dihydrostreptomycin on the column for the separation of the analytes from endogenous compounds. The aminoglycosides were detected and quantified with electrospray ionization tandem mass spectrometry.

方法用磷酸盐缓冲溶液提取蜂蜜中的链霉素和双氢链霉素后,经弱阳离子交换柱进行固相萃取,样品制备后采用亲水作用色谱,使链霉素和双氢链霉素实现保留,从而与内源性物质达到分离,采用电喷雾离子化串联质谱法检测和定量。

His story begins with Sumerian farmers who realised, some time in the third millennium BC, that the surpluses of grain generated within Mesopotamia's fertile crescent could be used as barter for things they did not have.

他的故事开篇描述的是大约在公元前3000年间,苏美农民意识到在美索不达米亚平原新月沃地上产出粮食的剩余部分可以用来进行物物交换,以获得自己没有的东西。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。