生长速率

As to Gd/Cr system,alower deposition rate of the Gd overlayer〓favors the smooth growth of Gd/Crinterface.

结果表明，低的Gd沉积速率(1.0〓/min)有利于Gd/Cr界面的平滑、均一的生长。

In the paper, the linear growth order of ammonium paratungstate crystal is studied by the "method of initial rate".

应用&初始速率法&研究了仲钨酸铵晶体的线生长级数。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Results The cells became larger and showed more pseudopodia, having atendency to differentiate, the detection of cell cycle showed a decrease of S stage andan increase of G1 stare, cell proliferation ability in vitro and tumorigenesis ability in nude mice were discounted, the final tumor volume and weight were also decreasedafter knocking down NS gene.

结果NS基因在PC-3细胞中的表达水平下调后，细胞胞膜边缘突起增多，更趋向于分化；S期细胞的百分率降低，G1期的百分率升高；细胞的体外增殖速率明显降低，在裸鼠体内致瘤能力、肿瘤的生长速度及肿瘤的最终体积和重量也明显降低，细胞凋亡增多。

The Rup2-saturated CO2 assimilation rate was 80.2 μmol m-2 s-1 for Rhodomyrtus, higher than those for Schima and Psychotria by 24.4% and 22.9%, respectively, suggesting that Rhodomyrtus have higher potential carboxylation.

光合作用的Rup2饱和速率80.2 μmol m-2 s-1，较荷木和九节分别高24.8％和22.9％，表明生长在疏林的桃金娘有较大羧化作用潜力。

Relative to nitrogen topdressing in prophase, it mainly due to retroposition can provide nutrients for the wheat during flowering and grouting. Another way, it can help slow down the decline in leaf area and leaf chlorophyll content and photosynthetic rate during later stage, to meet the nutritional requirements during developmental stage of grains.

结合以往试验结论，本试验推断认为由于追氮时期后移既有利于开花和灌浆阶段养分的需要，同时有利于延缓小麦生长后期有效叶面积、叶绿素含量和光合速率的急剧下降，从一定程度上满足籽粒发育阶段的营养所需。

Due to the forest litter layer is thicker than the grassland, its soil structure is looser and has higher water and organic matter content in the solum. In addition, the rhizomatic growth of forest community vertical structure has obvious differences on the herbosa in the pore size distribution and the infiltration rate.

由於林地较草地的枯枝落叶层为厚，使其土壤结构较松散，并会增加表层土壤水分保持及有机质含量，再者，森林及林下植物相较於草类根系的生长及分布情况，对於不同土壤深度之大小孔隙的比率及水分入渗速率具有明显的差异。

Ornithine decarboxylase, a rate-determining enzyme of the polyamine biosynthetic pathway, is involved in the control of cell cycle, differentiation, cell growth and apoptosis. Previous studies described that protein kinase Cd regulates ODC activity. In this thesis, human leukemia cell line, HL60 cell, was used for the treatment with a specific PKCd inhibitor, rottlerin, to measure the effects on cell growth.

鸟胺酸去羧化（ornithine decarboxylase，ODC）为多胺酸起始与决定反应速率的酵素，对细胞周期、细胞生长、分化及细胞凋亡扮演一重要角色；在寻找肿瘤治疗及预防药物研究中，纯化植物天然物质是其中来源之一，研究者筛选药物时，测定对鸟胺酸去羧化活性是否具抑制效果是一个重要的试验。

Saxicola. Phosphate was absorbed fastest, followed by nitrate and carbohydrate. It was suggested that phosphate and nitrate were the main compounds, which had effects on cell suspension cultures of C. saxicola.

结果表明营养物质的吸收与细胞生长以及生物碱的合成密切相关，营养元素的相对消耗速率为磷＞氮＞碳，推测氮和磷是影响岩黄连细胞培养的主要因素。

Under the visor net the sealwort average transpiration rate is open-air cultivates 81.4%, explained that the Taishan sealwort does not suit the open-air growth.

遮阳网下黄精的平均蒸腾速率是露天栽培81.4%，说明泰山黄精不适合露天生长。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。