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Result: It has been found out that the fat-soluble nonsaponified extract from Temminck et Schlegel and the alcoholic extract from Syngnathus acus have cytotoxic activities.The nonsaponified extract from Temminck et Schlegel can inhibit the grouth of cancer cell strains KB,Hela,PAA,K562,and Bcap37,and the alcoholic extract from Syngnathus acus can inhibit the growth of cancer cell strin KB,But Bloch shows no apparent anticancer activity.

结果:尖海龙的95%乙醇提取物对KB细胞的生长具有抑制作用,并呈量效关系;粗吻海龙的脂溶性非皂化物具有不同程度的抑制人癌细胞株KB,Hela,Bcap37,K562,PAA细胞的作用,并有明显的量效关系;拟海龙的各提取物未见有明显的抑制肿瘤细胞生长的作用。

The results showed that it adapted to saltmorphic circumstances through the following characters: A lot of aerenchyma existed in the vegetative organs;Phellem highly expanded in roots;thick cuticula;more mucilage cells and aleurone grains in parenchyma of roots and stems;A lot of water storing...

结果表明,不同生态环境中生长的马齿苋解剖结构显著不同,盐生马齿苋具有适应盐渍环境的结构特征,这些特征表现为:营养器官通气组织发达;根的次生结构中木栓发达;根、茎的薄壁组织中含有大量的黏液细胞和糊粉粒;叶片表皮的角质膜厚;叶肉中含晶细胞、叶绿体及贮水组织丰富;而这些特征是黑土地上生长的马齿苋所不具备的。

The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r/min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims:(1) controlling the penetrating pressure of the broth in the hypotonicity condition;(2) controlling the pH of the broth between 4.0 and 7.0;(3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure.

本发明以一株粪产碱杆菌为发酵菌种,使用食品工业中常用的蔗糖或葡萄糖作为微生物培养的碳源,以农业生产中常用的化肥作为氮源和无机盐,辅以酵母粉配制微生物生长的培养基,在30℃以1.0vvm、500r/min通风搅拌条件下好氧发酵,发酵过程中流加特殊配制的补料液以达到以下目的:(1)控制发酵液的渗透压处于低渗条件;(2)控制发酵液的pH处于4.0~7.0之间;(3)为微生物生长提供丰富的碳源和氮源等营养物质,经72小时得到威兰胶发酵液,在95℃~115℃下常压干燥得到威兰胶产品。

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的:研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2(MMP2)、环氧合酶-2(COX-2)表达的影响:方法:将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响,采用MTT法观察舒林酸对SKOV3细胞生长的影响,通过划痕试验观察舒林酸对SKOV3细胞运动的影响,利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的:研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2(mmp2)、环氧合酶-2(cox-2)表达的影响:方法:将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响,采用mtt法观察舒林酸对skov3细胞生长的影响,通过划痕试验观察舒林酸对skov3细胞运动的影响,利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

The microorganism method to solves the landscape hydrophila group entrophication has the good function,is obviously degenerate the water body COD,also can suppress the growth of the chlorophyceae plant effectively;④Chemistry method maintenance water quality may oxidize effectively, degenerate the pollutant and some organic matter in water,can effective kill the algae;⑤The clear silt can eliminate the death algae and each kind of organic and inorganic substance,thoroughly eliminate the environment which the algae grow,therefore the clear silt to algaes government is the very thorough means.

微生物法对解决景观水体的富营养化有较好的作用,对水体COD的降解作用较明显,能够有效抑制绿藻类植物的生长;④化学方法维护水质可以有效氧化、降解水中污染物和一些有机物质,能够有效的杀灭藻类;⑤清淤能够把死亡的藻类和各种有机和无机物质清除掉,彻底清除藻类生长的环境,所以清淤对藻类的治理是很彻底的办法。

For example, to CaSiO〓, wallastonite and cyclowallastonite could be found simultaneously in glazes between the temperature range of 1125℃±10℃, only wallastonite came out below the range, and only cyclowallastonite appeared above the range. Similarly, melilite structure and clinopyroxene structure connected with crystallization temperature. Usually, higher crystallization temperature contributes to forming melilite with simple structure and lower crystallization temperature contributes to clinopyroxene with complex structure.

如钙的偏硅酸盐,硅灰石、环硅灰石的转变温度区域为1125℃±10℃,组分适当的特定釉熔体,高于此温度出现环硅灰石,低于此温度出现硅灰石晶体结构;同样的,黄长石、辉石的出现也与温度有密切的联系,通常,晶体生长的温度较高有利于晶体结构简单的黄长石出现,晶体生长时温度较低有利于稍复杂的辉石结构的出现。

By analyzing the history to mortgage of Germany,the developing trend of mortgage in modern times and legislative conditions of Chinese mortgage ,suggestions are put forward on establishing current mortgage in china in the text,such as Perfecting register system, the independence of mortgage, mortgage of sequence fixed, mortgage conversible to instrument

内容提要:凭据对罗马法和德王法抵押权制度的历史、抵押权如今生长的趋向以及中国的抵押权立法状态的视察,提出了旨在促进市场经济的生长在中国创建流通抵押权的提倡:完满登记制度、抵押权的独立、抵押权序次的固定、抵押权的证券化。

Four substances including 2-methyl ethyl acetoacetate and barley straw extract distilled from bulrush as well as cetyl trimethyl ammonium bromide and isothiazo lintone were used for the comparison experiment in inhibiting the growth of Microcystis aeruginosa in various phases. Result showed that inputting chemicals in the lag phase has better effect than in the log phase, and adding in lag phase can effectively inhibit the algae growth. Although feeding allelopathic substance at log phase can lead to certain restraining effect, it cannot result in the effective inhibition of algae growth, which can be well achieved by feeding CTAB and isothiazo lintone below 10mgl/L during log phase.

研究用从芦苇中提取的2-甲基乙酰乙酸乙酯和大麦秸浸出液两种化感物质以及十六烷基溴化钱和异噻唑啉酮等四种药剂对不同生长期的铜绿微囊藻进行了对比抑制试验,结果显示在铜绿微囊藻生长的迟缓期投加试验药剂效果比在对数期投加效果都好,在迟缓期投加四种药剂,都有很好的抑藻效果;在铜绿微囊藻生长的对数期投加化感物质,虽然有一定的抑藻率,但效果较差,而在藻对数期投加10mg/L以下的CTAB和异噻唑啉酮能达到很好的水华抑制效果。

Objective: The aim of this study was to investigate the effects of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinon the growth of nude mice cervical cancer, and to explore the possible mechanism of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinin cells and molecules levels. The growth of human cervical cancer Hela cell, apoptosis of tumor cells , expression of CD31and PeNt were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling, and immunohistochemical staining. The purpose of this study is to provide a new way of medication to eure cervical cancer in clinic.

目的:本实验通过建立子宫颈癌裸鼠病理模型后,以此为研究对象,运用光镜、免疫组化及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术,观察人参皂甙Rg3[20—Ginsenoside Rg3]和顺铂单独及二者联合应用对人宫颈癌Hela细胞裸鼠移植瘤生长的影响,重点研究肿瘤的生长、肿瘤细胞的凋亡及PCNA、CD31的表达情况,以期从细胞及分子水平揭示人参皂甙Rg3和顺铂单独及二者联合应用对人宫颈癌Hela细胞的作用机制,为临床上药物治疗与预防宫颈癌寻找更加完善的途径。

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