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生长曲线

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The well-grown cells of the third passage were co-cultured in vitro on the nano-CS/COL scaffold. Taking simple nano-CS/COL scaffold material as control, the histocompatibility of scaffold material and cells were comprehensively evaluated by cell adherence rate, growth curve, cell activity and cycle, and scanning electron microscope observation.

取生长良好的P3代,与纳米壳聚糖-胶原纤维支架体外联合诱导培养,以单纯纳米壳聚糖支架材料为对照,通过细胞贴壁率、生长曲线、细胞活力及周期、扫描电镜观察综合评价材料与细胞的相容性。

Methods K562 cells were cultured and treated with HMBA and its derivatives. We studied their effect of inducing differentiation on K562 cells by benzidine staining and other methods often used.

细胞生长曲线绘制、集落形成试验,检测HMBA及其衍生物对细胞生长的影响,以Western blot检测p21、c-fos蛋白表达,以联苯胺染色显示血红蛋白。

In the growth curve experiment, it is found that TB has caused more significant growth inhibition in A431 human epidermoid carcinoma cells than in Hs695T and RPMI 7951 human melanoma cells.

生长曲线的实验我发现,相较於Hs695T和RPMI 7951这两株人类黑色素瘤细胞,TB抑制人类表皮癌细胞A431的生长效果较为明显。

Observation under Transmission Electron Microscope was carried out and growth curve of E. coli was ploted out. It was indicated that PSPPs might change the permeability of cell membrane, make cell excrescent and inhibit cell division.

透射电镜观察和大肠杆菌生长曲线表明,该色素的抑菌作用可能是通过增强细胞膜的通透性,使细胞异常生长,抑制对数生长期的细胞分裂,使细胞质稀薄、细胞解体。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌,进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线,其中NAC平板上筛选的4株拮抗菌的抗菌范围较广,锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

Using microbic growth curve,we can not only observe the growth curve and types of bacteria cultivating but also improve its positive ratio.

利用微生物生长曲线不仅可连续观察细菌生长分型,而且可明显提高细菌的培养阳性率。

The toxicity and the osteogenous ability on the surface of PCHA: the toxicity was examined by coculture with PCHA to record growth of cells with MTT method. The osteogenous ability was investigated by observation of the formation and growth of cells on the surface of PCHA bone cement.

PCHA毒性检测和表面细胞活性的观察:将PCHA浸出液与骨髓基质细胞共同培养,通过MTT比色描记细胞生长曲线,快速判定制孔剂添加后PCHA的毒性反应;将PCHA制成标准试件,与骨髓基质细胞共同培养,观察细胞形态和生长活性。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA,绘制细菌生长曲线,结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长,但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中,GlmU蛋白表达量无明显的变化。

The results of cell growth curves showed that allvoltages inhibited cell proliferation and prolonged cell multiplication time.

其中,生长曲线结果表明,所有的电压都抑制了 HeLa 细胞的生长增殖,延长了细胞的倍增时间。

Immunization of rabbitsUse suitable immunizing method to obtain antisera with high titer and highly activated spleen cells for fusion.

实验主要内容包括:(1)240E细胞的培养根据生长曲线的绘制,调整240E细胞生长状况,获得适合融合的240E细胞。

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