生长曲线

Methods Corneal epithelial cells of limbal, peripheral and centeral cornea were removed using improved enzymatic disaggnegation and cultured in vitro. The cells were counted in a hemocytometer at different time to determine the doubling time .

用体外细胞培养的方法，从生长曲线、细胞倍增时间、细胞DNA合成的活跃程度等几个方面，观察人角膜缘部、周边部及中央部角膜上皮细胞的生长特征。

Experimental results indicated that the silver nanoparticles of 20 μg/mL could inhibit completely the growth of 10^6 cfu/mL cells in liquid LB medium. The growth curves showed that silver nanoparticles prolonged the lag phase of E. coli, and the higher of the concentration of silver nanoparticles, the longer of the lag phase of E.

纳米银对大肠杆菌的抑制生长曲线的结果表明，20μg/mL的纳米银能够完全抑制10^6 cfu/mL的大肠杆菌细胞生长，纳米银使大肠杆菌的延滞期加长，并且纳米银浓度越高，延滞期越长。

In our study, we found menadione has anticancer potential on human breast adenocarcinoma MDA MB 231 by weather cell cycle arrest, anti proliferation, or apoptosis. Low concentration of menadione (5.8μM) significant inhibited growth curve on breast cancer cells (MDA MB 231) but not breast cells (MCF 10A) by performing MTT and trypan blue cell counting. RT-PCR and western blotting showed menadione increase p21 gene and protein expressions but decrease cyclin E and CDK2 protein expression resulting in cell cycle G0/G1 arrest by flow cytometry.

MTT及生长曲线的实验，发现menadione在低浓度且长时间作用情况下，对在於人类乳腺上皮细胞癌(MDA-MB 231)是有明显的抑制作用，而在正常乳腺细胞(MCF-10A)则没有影响，在分子转录上及蛋白质表现方面也发现低浓度menadione处理24时能使MDA-MB 231诱发p21基因及蛋白表现量增加及cyclin E、CDK2抑制，再经由流式细胞仪分析也发现低浓度 menadione 处理24小时能使MDA-MB 231细胞周期GO/G1比例上升，造成细胞周期停滞，进而抑制细胞生长。

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线，比较卵巢癌细胞系HO－8910与8910－p93、8910一pCDNA3.1（转染空载体ncDNA3.l）的增殖情况；通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力；利用流式细胞仪检测这3种细胞的细胞周期的改变情况：利用透射电镜观察转染p93基因的细胞的超微结构变化；利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

The normal cell growth curve of the transduced lymphocytes indicated that these cells could survival for long periods and be strained.

细胞计数测定细胞生长曲线，结果表明转染的淋巴细胞能长期存活和建株，其本身生长不受影响。

Methods:Preparing pastille serum of Rehmanniae pill of Six Ingredients with low,medium and high doses and using 10% pastille serum with different doses to cultivate OS-732 cells.

制备低、中、高剂量的六味地黄丸含药血清。10%低、中、高剂量的六味地黄丸含药血清培养OS-732细胞，显微镜下观察细胞生长情况；进行细胞计数和绘制生长曲线；用MTT法检测细胞增殖；进行细胞碱性磷酸酶活性测定。

Furthermore, the analysis of X- ray diffraction diagram indicated that cadmium ion was transformed into cadmium sulfides by H strain. Also, we found that the removal for cadmium was dependent on the growth stationary phase of H stain through determining the removal rate for cadmium, the growing curve of cell and the activity of cysteine desulfhydrase.

通过X射线衍射光谱分析，H菌株对镉离子的转化产物为硫化镉，通过测定镉的去除与细菌生长曲线及半胱氨酸脱巯基酶的活性，表明镉的去除与菌体的生长稳定期相关。

Cell grew slowly in 48 hours, and spindle and polygon-shaped cells were observed after 72 hours.②The growth curves of MSCs in C/GP were shown as a sigmoidal shape.

绘制MSCs生长曲线为"S"形，第1个24 h为细胞潜伏适应期，第2~3个24 h为对数生长期，4 d后细胞增殖减慢，5 d后细胞基本停止生长。

Rape ; pod ; seed ; growth curve of Stype ; straight-growth period ; peak-growth period

油菜；角果；种子； S型生长曲线；直线生长期；生长高峰期

The biological characters of p5CS-bar gene transgenic wheatgrasse and control plants were observed and analysed that none but caudex have marked differences(0.05); The values of plant height、spike length、spike width and stem/leaf are less than control plants, leaf length and leaf width are more than control plants; Under the same condition of growth and climate to compare transgenic wheatgrass with control plants we can see that trning-green sage is advance, defer procreate period, long viridity period, growth speed become slow and the figure of curves is"S", more tillers.

2对转p5CS-bar基因冰草阳性植株和阴性对照植株进行生物学性状观测比较，分析得出：只有丛茎差异显著，显著水平为0.05；株高、穗长、穗宽和茎叶比的值均稍小于对照，叶长和叶宽的值稍大于阴性对照；在栽培、气候相同条件下，返青期相对提前，生育期推后，青绿期延长；生长速度变缓，在整个生育期呈"S"型生长曲线；分蘖能力增加。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。