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生长曲线

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Trichophyton rubrum ; growth curve ; characteristics of sporulation ; rDNA sequence

红色毛癣菌;生长曲线;产孢特点; rDNA序列分析

PHBV was implanted subcutaneously in rabbit and extirpated at 2,4,6,8, and 10 weeks in order to study its biocompatibility and biodegradability.

体外培养犬主动脉瓣间质细胞,观察其生长曲线,平滑肌α肌动蛋白表达和透射电子显微镜下特点。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞,并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上,当细胞接近融合时,及时取出作原位甲醇固定,CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞,以5×10~4/ml密度传代、分别培养1-9天,每天分别细胞计数并进行分析比较,重复3次。

Study in vitro The chondrocytes of SD rat were isolated from epiphyses by collagenase Ⅱ and the cultured chondrocytes were confirmed by HE, toluidine blue, alcian blue staining, and by IHC with anti-collagen type Ⅰ and Ⅱ.

体外实验 SD大鼠骺软骨细胞分离培养,HE、甲苯胺蓝、标准阿尔新蓝染色及Ⅰ、Ⅱ免疫组化染色鉴定,绘制细胞生长曲线

Moreover, it was found out that the status of gas-producing was related with the growth curve of four bacteria strains selected from the sludge.

此外,废水产氢规律与从污泥里筛选出的产氢菌生长曲线有密切的关系。

Fermentation condition was researched. Growing curve of lactic acid bacteria ATCC15019 and ACE-inhibitory activity of the products was measured. We used proteinase to hydrolyse whey proteins, and the product can be used to measure ACE-inhibitory activity.

本实验的主要内容如下:用MRS培养基培养瑞士乳杆菌ATCC15019,测定其生长曲线,并研究发酵牛奶过程中发酵液抑制ACE的变化,确定此菌适合用来进行乳酸菌蛋白酶乳清水解物抑制ACE活性的研究。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间,进行髓核细胞活力测定;爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察;MTT 法绘制髓核细胞生长曲线,并行原代及第2 代细胞透射电镜观察,对体外细胞的生物学特性进行研究。

The results showed that Gompertz model is much more simulated the observed data than Logistic model and line model at 4℃, 7℃.

经过t检验和F检验过程验证生长曲线样本,Gompertz模型被接受度最好,t检验达到100%接受度,而F检验达到83%。

The results showed that biomass accumulation of maize plants accorded with the S-shaped curve as plants developed.

结果表明,玉米植株干物质积累与生育进程的关系呈S型生长曲线;在群体干物质积累速率和最终积累量都表现出先玉335高于郑单958及长城799的趋势。

Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method.

测定其接种贴壁率,在倒置显微镜下连续观察细胞的形态变化,利用甲基噻唑基四唑(methyl thiazdyl tetrazolium, MTT)法测定 MSCs 生长曲线,并应用流式细胞术测定细胞表面标记物。

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