生长曲线

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

Weretrospectively analysed 539 patients'microbic growth curve.

对539例患者微生物生长曲线进行回顾性分析、分型。

MAIN OUTCOME MEASURES: Cell morphous was observed with an inverted microscope to draw growth curve.

主要观察指标：倒置显微镜下观察细胞形态，绘制生长曲线。

The Taenia solium oncosphere 18 gene was cloned and reconstructed into expression vector pYA3341.The recombinant plasmid pYA3341-TSOL18 was finally electro-transformed into an attenuated S.typhimurium definitive host strain of X4500 and the recombinant strain of X4500 (pYA3341-TSOL18) was identified its immunogenicity of expression protein, stability, growth curve, safety in vitro and immunity evaluation by animal experiment in mouse.

克隆并改造TSOL18基因，构建重组质粒pYA3341-TSOL18，电转入鼠伤寒沙门氏菌终宿主菌株X4550，体外鉴定重组菌X4550(pYA3341-TSOL18)表达蛋白的免疫原性、稳定性、生长曲线、安全性和小鼠免疫试验进行评价。

The toxicity and the osteogenous ability on the surface of PCHA: Cytotoxicity tests demonstrated that PCHA has excellent biocompatibility, and cells grow normally on the surface of PCHA.

CHA毒性检测和表面细胞活性的观察：PCHA浸出液与骨髓基质细胞共培养，MTT检测细胞生长曲线与空白对照组无差异，表明材料无毒性。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

AIM: To investigate the antineoplastic effect of pholiota nameko polysaccharides and the mechanism of inducing K562 cell apoptosis. METHODS: MTT assay was used to determine the inhibitory rate of PNP with different concentrations on the proliferation of K562 cells, and the cell growth curve was drawn.

采用MTT法检测PNP对K562细胞增殖的抑制作用；绘制生长曲线；Hochest 33258染色计算凋亡率；RTPCR法、Western Blot方法检测凋亡相关基因Caspase3的表达。

Second, the time of the saccharomycetes which muted is 14～16hours.

在突变株发酵性能实验中，通过对酵母菌生长曲线的绘制和流加时间的确定，得到诱变后菌种的最佳培养时间为14～16小时。

Saxifraga stolonifera Meerb ; Ethanol extract ; Growth curve ; Antimicrobial effect

虎耳草；乙醇提取物；生长曲线；抑菌作用

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。