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生长曲线

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The growth curve showed proliferative phase 3~8th days, top-plate phase 9~10th days and attenuative phase after 10th days.

细胞生长曲线,增殖期3~8天,平顶期9~10天,衰亡期10天以后。

Doubling rate was fastest in the growth curve of autoserum group, followed by homogeneity foreign serum group and fetal bovine serum group. However, no doubling phenomenon was found in the DMEM group.

在细胞生长曲线中大鼠自身血清组倍增速率最快,其次为同种异体血清组,再者为胎牛血清组,而DMEM组细胞未见明显倍增现象。

By adding pre-existing data for African pygmies to newinformation they have collected about the Aeta and the Batak of thePhilippines, they show that at the beginnings of their lives all these pygmypopulations follow the same growth curves as taller people, including Turkanaand Americans.

通过收集已存在的非洲矮人的数据和菲律宾的Aeta和Batak,他们发现在侏儒一生的早期,所有的这些侏儒与更高的人,包括Turkana和美国人遵循同样的生长曲线

Retrieving capsulated cells for cell counting, MTT assay,CCK-8 assay daily for 8days to determine cell activity,and analysis growth curve.

囊管内细胞胰酶消化回收,以细胞计数、MTT法、CCK-8法测定细胞的活性,并进行生长曲线分析。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组,设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植,囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性,共8天,进行生长曲线分析。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明,石榴果皮提取物主要是通过抑制HMP途径,导致还原力NADPH缺乏,引起代谢功能障碍,达到抑菌的目的;加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明,石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂;SDS-聚丙烯酰胺凝胶电泳分析表明,石榴果皮提取物能够抑制菌细胞蛋白合成,尤其对菌体内大分子量蛋白作用最明显。

Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

结果 骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞,经过生长曲线分析,二磷酸盐和骨碎补总黄酮10^(-4)~10^(-6)mmolL^(-1)时,对诱导后的成骨细胞有抑制作用;10^(-8)molL^(-1)有促进作用;10^(-10)~10^(-12)mmolL^(-1)不明显,其中10^(-8)mmolL^(-1)作用最强;基因分析后,发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP,且两者合用效果大于单独用药。

Their dynamic morphological changes,growth curve,glycerophosphate dehydrogenase pattern,oil red O staining,and reaction to insulin and dexamethasone all verified their preadipocyte identity.

经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红O脂肪染色及对胰岛素和地塞米松反应的测定,证明是功能活跃的前脂肪细胞,并在体外重现了其增殖、肥大的全过程。

There were significant differences in theproliferation activity day 8 and day 10 compared with that of day 5 and day 14 inculture.

细胞生长曲线呈&S&形,8天和10天的增殖活性强于5天和14天。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖,使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长,光镜下见细胞突起变短,细胞由梭形变成椭圆形,核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

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