生长曲线

Methods: Soft agar clone forming test, cell morphology, scratch test, MIT and flow cytometry were used to examine the changes of Hep-2 cells treated by different concentration of PB in vitro. Results: There was a dose-dependent and time-dependent inhibition of the cell proliferation by PB.

应用细胞生长曲线测定法、软琼脂克隆形成试验、细胞形态观察、划痕试验、MTT法、流式细胞术等对在体外经不同浓度PB处理过的Hep-2细胞进行检测，观察细胞分化程度及运动能力的改变。

The cells were treated with taxol or/and radiation. Growth curves were drawn and sensitizer enhancement ratios were evaluated.

2细胞经紫杉醇和照射处理后，绘制细胞生长曲线，计算出10%存活率时的放射增敏比。

P for short at different temperature was studied by using the method of predictive microbiology. Growth curves were obtained at 4~40℃. and foud that the Gompertz model fit at 10~40℃ with representation of experimental curves which are typical sigmoidal.

结果表明，在10~40℃，副溶血性菌弧的生长曲线呈典型的S形，适合用Gompertz和logistic模型拟合；当温度在4~10℃时，适合用线性回归方程拟合。

Methods 252positive samples of blood culture were an-alyzed according to the lag phase,loganithmic growth phase and stationary phase of bacterial growth curve.

方法将血培养阳性标本252例，按照细菌的生长曲线的三个阶段--迟缓期、生长期、稳定期进行分析犤1犦。

Growth curves of CFs were established by cell counting and methyl thiazolyl tetrazolium.

细胞计数和MTT法作出CFs的生长曲线，检测CFs的增殖。

METHODS: The MGC-803/ E2F-1 gastric cancer cells which were stably transfected with E2F-1, and containing unpossessed carriers, and the cells without transfection were performed MTT test to draw growing curve, and to compute cell proliferation rate. Cell cycles were examined by flow cytometry.

对稳定转染E2F-1的胃癌MGC-803/E2F-1细胞、转染空载体的MGC-803/EV及未转染的MGC-803细胞进行MTT法检测，绘制生长曲线和计算细胞增殖率；流式细胞仪检测各组细胞周期分布。

Methods The embryo calvarial periosteum tissue was taken and, human osteoblasts were obtained by enzyme-assimilating methods. The morphological change, growth feature and osteogentic capability of osteoblasts were observel during culture in vitro, drew the growth curve was graphed and the cells was identified by alkaline phosphatase dye. At the same time, the morphology and bioactivity of 3 to 5 th-generation osbeoblasts and anabiotic cells were studied comparatively.

取人胚胎颅骨骨膜，采用酶消化法获取成骨细胞体外培养并传代，观察细胞形态，生物特性，绘制生长曲线，并经碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3~5代与冻存后成骨细胞的特点。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

MTT assay and morphological observation were used to evaluate the toxicity of rotenone on astroglia,cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RTPCR.

观察染毒星型胶质细胞形态，检测细胞活力，通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RTPCR检测缝隙连接蛋白(connexin43，CX43)mRNA表达。

MTT assay arid morphological observation were used to evaluate the toxicity of rotenone on astroglia, cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RT-PCR.

观察染毒星型胶质细胞形态，检测细胞活力，通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RT-PCR检测缝隙连接蛋白(connexin43， CX43)mRNA表达。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。