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Correlation of histotypes of phyllode tumor with local recurrence and tumor death was statistically significant at a level of P<0. 001; correlation of infiltrative growth of the tumor with local recurrence was statistically significant at a level of P<0. 001. Tumor necrosis and mitotic activity were independent prognostic factors.

叶状肿瘤的病理组织学等级与肿瘤的局部复发和因瘤死亡有统计学线性关系(P<0.01);肿瘤的浸润性生长方式与肿瘤的复发有线性关系(P<0.01);肿瘤性坏死和核分裂数值是独立的预后因素;术式等11个临床病理因素为单风险预后因素。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)有较好的抑制生长作用,ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml,对肝癌细胞hepg2抑制作用差, ic50为0.937 9 mg/ml;白花丹石油醚提取部位对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml;乙酸乙酯提取物对肺癌细胞(lu-04)有一定的抑制作用,ic50为0.734 3 mg/ml;水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好,值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

Diabetic retinopathy is one of the most serious microvessel complications of diabetes,it is a main cosmopolitan blindness disease. In recent years, with the molecular biology development, the recognition of the mechanism of DR is more profound and consummate. Most researchers consider that hyperglycaemia, polyalcohol inositol metabolism abnormality, changes of hemorrheology, oxidative stress, inflammation/immune system activation, cytokines/growth factors expression or activity change and so on result in DRs onest and development.

糖尿病性视网膜病变(diabetic retinopathy, DR)是糖尿病最严重的微血管并发症之一,也是一种世界性的主要致盲眼病,近年来随着分子生物学方面的研究进展,对DR发病机制的认识进一步深入和完善,认为高血糖、多元醇肌醇代谢异常、血液流变学改变、氧化应激、炎症/免疫系统活化、细胞因子/生长因子表达或活性改变等导致DR的发生发展。

Although the primary human hepatocytes would be ideal for bioartificial livers, the shortage of hepatocytes and the difficulty to increase the doubling time of human hepatocytes in vitro limit the clinical application.

肝细胞是生物人工肝支持系统的核心部分,从生物工程学角度讲,理想的用于生物人工肝的肝细胞要人源性、表型正常,容易获得和培养,能在体外迅速生长增殖至高密度,保持良好的分化状态,具有成熟肝细胞的所有生物代谢功能。

The purpose of the operation is to correct rachis side turn, increase the stability of rachis.

概要: 特发性脊柱侧弯是指不明原因的脊柱偏离正中线,形成向左或向右的不同程度的弯曲畸形,多发于青少年,影响患者的生长发育和体形,胸段侧弯可致心脏移位、心肺功能不全、甚至截瘫[1],造成心理生理上的损害。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标,从专业角度构建最优序列,经灰色分析和不同菌株毒力效价显著性比较,筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038,对甜菜夜蛾的校正死亡率分别为88.33%和86.70%,僵虫率分别为86.67%和85.27%。

Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测,提示传代的细胞均一性较好,表面抗原CD34表达阴性,CD44表达阳性的细胞均在95%以上,说明得到的是比较纯化的MSCs。

Furthermore, several bacteria genera are anaerobic or facultative anaerobic, and therefore cannot form pure cultures by ordinary streak plate method. Combination of well developed classical anaerobic microbiological techniques with modern molecular techniques, along with dynamic understanding of growth status and mode of odor production by different bacteria genera is critical for successful future studies.

此外, 猪粪尿排泄物中很多微生物是厌氧或兼性厌氧菌,无法通过普通的平板划线法而获得纯培养,今后应将传统的微生物厌氧培养方法与现代微生物分子生物学技术结合,动态了解和掌握不同菌群的生长状态及在恶臭产生过程中的作用。

The irregular variation of lattice constants of the a-LiIO3 single crystal originated in the growth process and the fluctuation phenomena of lattice constants under the action of an electrostatic field were investigated by an X-ray double-crystal spectro-meter in the arrangement.

本文用X射线双晶衍射平行排列的方法,观察了α-LiIO_3单晶体生长过程点阵常数a与c不规则的不均匀性以及在静电场作用下c的起伏现象。观测出在静电场作用下a与c的变化和沿电场方向晶体表面层存在点阵常数的梯度。

To explore the feasibility of the mitochondrial DNA as transgenic vector, we translated the mtDNAs of the Antheraea pernyi, Diaphania pyloalis Walker and the fish to the silkworm. Through microscopic examination and mtDNA polymorphism analysis, we tested the maintenance of the foreign mtDNA in the silkworm and investigated the influence of the foreign mtDNA to the physiological behavior.

为了探索线粒体DNA作家蚕转基因载体的可行性,本实验将柞蚕、桑螟和鱼的线粒体DNA转入家蚕体内,通过着色镜检、线粒体DNA多态性分析检测了外源线粒体在家蚕细胞中的存留情况,并调查了外源线粒体对家蚕生长的影响。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?