生长性的

The edible parts of the vegetables(stems of water dropwort and corms of arrowhead and water chestnut)showed enrichment of 147 Pm with the absorption coefficient,being 1.29±0.28,2.09±0.03 and 1.55±0.55,respectively.

其中，水芹菜随生长时间的延长，吸收147Pm的量明显增加，50、80、110d时全样的吸收系数分别为0.98±0.19、0.92±0.38和1.14±0.18；(2)3种蔬菜的食用器官(水芹菜的主茎，慈姑和荸荠的球茎)对147Pm具有明显的富集性，吸收系数分别为1.29±0.28、2.09±0.03和1.55±0.55。

In order to improve the reliability of the nuclear Generating Sets and the stabilization of the power system, prevent the long time low frequency oscillation caused by the disturbed of the outside electrical net, assured improving the stabilization margin of the nuclear generating sets in the precondition of enough transient stability, validate the sensitivity of the parameter to the damping of Generating Sets of the MEC5220 digital excitation adjustor produce in Japan that used in the Second Project of Qinshan Nuclear Power Plant, confirm the parameter rationality of the digital excitation adjustor, optimize the parameter of PSS, in this paper, we put up a in-depth study to the principle of MEC5220 digital adjustor produce in Japan by the PSS/E software that develop by the PTI Company in the USA, completed the modeling of the excitation adjustor, PSS, generator, excitor, described the performance of this excitation adjustor, analyzed the essential reason that caused the low frequency oscillation, illuminated the factor that influence the damping characteristic of the MEC5220 digital excitation adjustor. Based on model and emulate, this paper optimized the primary parameter of the excitation adjustor, make a reasonable regulation to the PSS parameter.

为提高核电机组的运行可靠性和接入系统的安全稳定性，防止在外电网的干扰下核电机组发生长时间的低频振荡，保证机组在暂态稳定的前提下提高其稳定裕度，验证秦山核电二期核电机组所配日产MEC5220型数字式励磁调节器参数对机组阻尼的灵敏性，确定数字式励磁调节器的参数的合理性，优化PSS整定参数，本文利用美国PTI公司研发的PSS／E软件对日产MEC5220型数字调节器的原理进行进行了深入细化的研究，完成了励磁调节器、PSS、发电机、励磁机的建模，描述了该型励磁调节器的性能，分析了产生低频振荡的根本原因，阐明了影响阻尼特性的因素。

Positive significant correlation between three infection indexes and SDD, and negative insignificant correlation between infection indexes and LIP were found. Fifteen sugarcane varieties could be divided into 6 groups on the basis of the distance more than 1.0. It resulted most ot tested sugarcane varieties had similar smut resistance. It suggested that smut race 1 and race 2 were included in inocula and the condition for Ustilago scitaminea infection was suitable in the crop season by setting up the standard varieties of NCo310, F134, NCo376 and Ya71-374 for smut identification. In addition, the results of sugarcane varieties resistant to smut race 1 and race2 were known by only a simple field trial.

结果显示：9个引进甘蔗品种中，ROC26属于感病品种，其余属于抗病或高抗品种；3个病情指数和持续发病期的两两相关均为显著正相关，潜伏侵染期与这些参数的相关为负相关，但未达到显著水平；在类间距离大于1.0的条件下，可将15个品种聚为6类，进一步明确了各品种抗黑穗病性的相似程度；抗性鉴定标准对照种NCo310、F134、NCo376和Ya71-374的应用，明确了接种源为小种1和小种2，通过一次接种试验，明确了供试品种对2个小种的抗性水平，标准对照种的抗性表现，还说明了本生长季发病条件基本是适宜的。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The cultural condition of PGPR strains Temperature,pH,light,cultural method,acid or alkali producing,and salt tolerance of 9 PGPR strains isolated from Gramineous Forage were tested,the results showed that all the PGPR strains could grow in the temperature range from 5℃～45℃,and optimum temperature for 178,O-6,Dry6 strains was 35℃,for X5,173,Y5,C-6 strains was 30℃,for N4 strain was 25℃～35℃,for 86 strain was 25℃;most of PGPR strains prefered to neutral or alkaline condition,strains 178,O-6,N4 and X5 were preferable to alkali condition especially;light was beneficial to PGPR's growth;all of them produce alkali;most of PGPR strains were not sensitive to NaCl concentration;all the strains were aerobiotic bacteria.

结果表明：各供试菌株对温度的适应范围较广，在5℃～45℃范围内均能生长，178、O-6、Dry6 菌株的适宜生长温度为35℃，X5、173、Y5、C-6 菌株的适宜生长温度为30℃，N4 菌株的适宜生长温度为25℃～35℃，86 菌株的适宜生长温度为25℃；大部分菌株在中性或偏碱性的条件下生长好，特别是Dry6、N4 和X5 菌株对碱性环境适应性强，在pH 值8.0 时生长最好；光照有利于菌株的生长；绝大部分菌株在3%NaCl浓度下生长良好，在5%～7% NaCl 浓度下除173 和86 菌株外，其它菌株都能生长，即对盐份的耐受性较好，且9 个菌株均为产碱菌；除173 菌株是兼性厌氧细菌外，其它都是好氧性细菌。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The research result showed that(1) The reclaimed water used didn't influence the sensory quality of a given turf grass tested;under irrigation of reclaimed water,Festuca arundinacea are the best,followed by Buchloe dactyloides and Zoysia japonica;(2) The influence of different types of irrigation water on growth characters of turfgrass is species-dependence;(3) Irrigation by reclaimed water and fresh+reclaimed water increases Na~+,CEC,and total porosity of the turf soil in comparison to fresh water,and improves soil quality.

结果表明：本研究使用的再生水对所供测试绿化草种的颜色、覆盖度、质地和均匀性无不良影响，再生水灌溉条件下，高羊茅表现出了最强的综合耐污能力，其次是结缕草和野牛草。灌溉水类型对于草坪草的生长指标的影响具有种的特异性。再生水灌溉和&清水+再生水&交替灌溉提高了土壤的阳离子交换量和总孔隙度，有利于土壤物理性状的改善。

Itamin D from the skin and diet is metabolized in the lier to 25-hydroxyitamin D (Figure 1), which is used to determine a patient's itamin D status1,2,3,4; 25-hydroxyitamin D is metabolized in the kidneys by the enzyme 25-hydroxyitamin D-1-hydroxylase (CYP27B1) to its actie form, 1,25-dihydroxyitamin D.1,2,3,4 The renal production of 1,25-dihydroxyitamin D is tightly regulated by plasma parathyroid hormone leels and serum calcium and phosphorus leels.1,2,3,4 Fibroblast growth factor 23, secreted from the bone, causes the sodium–phosphate cotransporter to be internalized by the cells of the kidney and small intestine and also suppresses 1,25-dihydroxyitamin D synthesis.5 The efficiency of the absorption of renal calcium and of intestinal calcium and phosphorus is increased in the presence of 1,25-dihydroxyitamin D (Figure 1).2,3,6 It also induces the expression of the enzyme 25-hydroxyitamin D-24-hydroxylase (CYP24), which catabolizes both 25-hydroxyitamin D and 1,25-dihydroxyitamin D into biologically inactie, water-soluble calcitroic acid.2,3,4

从皮肤和食物来的维生素D在肝中代谢为25-羟基维生素D（图1），被用来决定病人体内维生素D情况的1，2，3，4；25-羟基维生素D在肾中被25-羟基维生素D1羟化酶（CYP27B1）转变为有活性的1，25-二羟基维生素D 。1，2，3，4由肾产生1，25-二羟基维生素D是被血浆甲状旁腺激素和血清钙，磷水平紧密调节。1，2，3，4由骨分泌的成纤维细胞生长因子23使钠磷协同转运蛋白被肾和小肠细胞内化及抑制1，25-二羟维生素D合成。5 在1，25-二羟基维生素D作用下肾和小肠吸收钙及磷的效率增高(图1)。2，3，6 它也包括25-羟四- 24 -羟化酶的表达(CYP24)，且将1，25二羟基维生素D和25羟基维生素D异化成无生物活性，水溶性的维生素D3-23羧酸。2，3，4

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

Therefore, in the recent years, queensland nut is quite popular in all over the world with continuously increasing price, and it has become the most popular high-rank nut on the international market.

在适宜的条件下，澳洲坚果定植后6－7年即可获得相当可观的产量，丰产期达40－60年，甚至更长，生长良好的健康树，其丰产性随着树龄的增长和植株增大而增长。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐