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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中,苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变,并筛选在自生生长时期、共生生长时期的表型,以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因,我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法,发现lsrA基因主要在根瘤侵染区开始表达,表达时序也在侵染阶段左右,但晚于bacA基因表达;LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体,nifA和fixK基因的转录水平降低,nifH基因的转录被完全阻断,因此LsrA蛋白为根瘤发育所必需,是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因,并研究了它的调节特性。oxyR突变后,苜蓿中华根瘤菌对过氧化氢敏感性提高,适应性降低。

In this study,we focus on the interaction between nuclear modifier gene MTO2 and aminoglycosides,and have received the following innovations.1,We here report first that the knockout of MTO2 significantly inhibited mtDNA p_(454)~R mutant's sensitivity to aminoglycoside antibiotics.Here are the results:(1)In neomycin GYP medium,when mitochondria genes were wild type,whether MTO2 knockout or not,the growth of yeast strains has no significant difference;when mitochondria genes were complete deletion,whether MTO2 knockout or not,the growth of yeast strains also has no significant difference;Interesting,when mtDNA P_(454)~R,wild-type MTO2 strains were hardly survival in neomycin GYP medium,but the growth of MTO2 knockout strains were only partly inhibited,there was a significant difference performance of neomycin sensitivity between them.

具体结果如下:(1)在含有新霉素的葡萄糖培养基上,mtDNA为野生型、MTO2敲除与否,相应的酵母菌株的生长没有显著差异;mtDNA完全缺失、MTO2敲除与否,相应的酵母菌株的生长也没有显著差异;然而,当mtDNA存在P_(454)~R突变时,MTO2野生型菌株几乎完全不能生长,而MTO2敲除菌株的生长只受到部分抑制,表现二者对新霉素的敏感性存在显著差异。

Results showed that the growth of N. sphaeroides was inhibited by low temperatures, most severely, it was nearly ceased at 2℃ and 5℃, while, at 10℃, and normal growth was attained after a prolonged lag phase.

结果表明,低温抑制葛仙米的生长,在2℃~5℃低温下其生长甚至完全停止;在10℃低温胁迫下,其生长延滞期变长,其后仍能保持正常生长。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长,以少突胶质细胞表现更为明显;(2)星形胶质细胞呈向心性生长,细胞相邻的接触面有较多的伪足;(3)传代培养的星形胶质细胞保持较强的分裂增殖能力,可在1周左右生长至完全融合,其纯度可达95%以上。

It's found that the (001) facet is the growth facet when the NBW crystal is grown by the Bridgman method through spontaneously crystallization. This phenomenon is in accordance with the growth characteristic of the typical scheelite crystals, and can be well explained by the Bravais law and the theory of anion coordination polyhedron.

指出坩埚下降法自发成核NBW晶体生长的生长面为(001)面,这与白钨矿结构晶体(001)面生长快的特点是完全符合的,Bravais法则和负离子配位多面体生长基元理论都能够很好地解释这一现象。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根据在0.5、1.4、50、100 μg/ml等不同浓度的含药PSA平板上能否生长,将玉蜀黍赤霉田间菌株对多菌灵的敏感性划分为:敏感、中抗和高抗等3个水平,其中S菌株在0.5 μg/ml浓度下能生长,但在≥1.4 μg/ml浓度下生长受到完全抑制;R菌株在1.4 μg/ml浓度下能快速生长,在50 μg/ml浓度下能缓慢生长,但在≥100 μg/ml浓度下不能生长;HR在≥100 μg/ml浓度下仍能生长。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验,结果显示,0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu/mL以下的革兰氏阳性短芽孢杆菌的生长;1‰以下的山梨酸钾不能完全抑制5×10~4 cfu/mL革兰氏阴性粗短杆菌的生长,对10~4 cfu/mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu/mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长,能明显的抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株生长,对10~5 cfu/mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu/mL革兰氏阴性螺旋菌菌株的生长;能明显抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株的生长,而20 mmoL EDTA能很明显抑制10~5 cfu/mL肠杆菌菌株的生长。25mg/mL、50 mg/mL、75 mg/mL和100 mg/mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用,但抑制程度不同,抑菌活性有一定的变化。

In the whole growth period of spring wheat, the response was different in yield and phase growing on climate change, such as the appearing time of sprouting, jointing, tasselling and maturity being ahead but milking being postponed. That is to say, it shortened the spring wheat's nutrition growth stage of seedingsprouting, elongated the sproutingjointing stage, cut down the generative growth in the jointingtasselling phase, prolonged the tassellingmilking stage, and shortened the milking~maturity stage. These finally resulted in the shortening of whole growth period.

在春小麦整个生长发育过程中,其阶段生长和产量对气候变化的响应不完全一致:气候变化使该地区春小麦出苗期、拔节期、抽穗期和成熟期的出现时间均提前,而乳熟期出现时间推迟,导致播种—出苗期的营养生长阶段缩短、出苗—拔节期的营养生长阶段延长、拔节—抽穗期的生殖生长阶段缩短,抽穗—乳熟期的生殖生长阶段延长,乳熟—成熟期的生殖生长阶段缩短,最终造成全生长期缩短。

In the whole growth period of spring wheat, the response was different in yield and phase growing on climate change, such as the appearing time of sprouting, jointing, tasselling and maturity being ahead but milking being postponed. That is to say, it shortened the spring wheat's nutrition growth stage of seeding-sprouting, elongated the sprouting-jointing stage, cut down the generative growth in the jointing-tasselling phase, prolonged the tasselling-milking stage, and shortened the milking-maturity stage. These finally resulted in the shortening of whole growth period.

在春小麦整个生长发育过程中,其阶段生长和产量对气候变化的响应不完全一致:气候变化使该地区春小麦出苗期、拔节期、抽穗期和成熟期的出现时间均提前,而乳熟期出现时间推迟,导致播种-出苗期的营养生长阶段缩短、出苗-拔节期的营养生长阶段延长、拔节-抽穗期的生殖生长阶段缩短,抽穗-乳熟期的生殖生长阶段延长,乳熟-成熟期的生殖生长阶段缩短,最终造成全生长期缩短。

Result A strain of Pseudomonas cepacia was detected in 1.6% compound quaternary ammonium disinfectant before application. The amount of the germ was 1.21×105cfu/ml. The strain had the similar resistance to chlorine-containing disinfectants and glutaraldehyde compared with Staphy aureus. The contaminated disinfectant could still thoroughly destroy the antigenicity of HBsAg with 60min's contact after diluted for 5-20 times.

结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近,污染样液除菌后1 000倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果,20倍稀释可完全破坏HBsAg抗原性,和抑制枯草杆菌黑色变种芽胞的生长,1 600倍稀释可抑制白色念珠菌生长,而原液则不能抑制分离菌株的生长。

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推荐网络例句

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。