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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

The results showed that the strain HH407 was gram negative, rod shaped, aerobic, with a single polar flagellum, neither endospore nor capsule formed, about 0.4 ~0.7 μm×1.0~1.8 μm in size.

对从连云港海域筛选的一株产低温碱性蛋白酶的海洋适冷菌HH407进行的鉴定和生长特性研究结果表明,该菌为革兰氏阴性杆菌,好氧,端生鞭毛,无芽孢及荚膜,大小为0.4~0.7μm 1.0-1.8μm,在酪蛋白培养基的菌落特征为椭圆金黄色、光滑、半透明、四周隆起。

A strain HH407 which could production cold-active alkaline protease, was isolated from the sea area of Lianyungang. Then, the identification and physiological characterization of the strain HH407 were studied. The results showed that the strain HH407 was gram negative, rod shaped, aerobic, with a single polar flagellum, neither endospore nor capsule formed, about 0.4~0.7 μm×1.0~1.8 μm in size. The colony on screening medium were circular, yellow color, smooth, translucent, convex with regular edges.

对从连云港海域筛选的一株产低温碱性蛋白酶的海洋适冷菌HH407进行的鉴定和生长特性研究结果表明,该菌为革兰氏阴性杆菌,好氧,端生鞭毛,无芽孢及荚膜,大小为0.4~0.7μm×1.0-1.8μm,在酪蛋白培养基的菌落特征为椭圆金黄色、光滑、半透明、四周隆起。

Although underlying mechanisms ⑴ hae not been elucidated, eidence points to the participation of the nitric oxide and cyclic guanosine 3',5'-monophosphate system.

虽然根本的机制还没有阐明,但是证据显示一氧化氮和环鸟苷酸系统参与其中。这里,我们检测到在MC3T3-E1成骨细胞的分化中基质金属蛋白酶13的mRNA,蛋白和活性的升高,诱生一氧化氮合酶和一氧化氮产物的升高。

There are six kinds of fatty acid in the grey green pilius and mycelia. The content of setearic acid in fruitbody is high ,which is 56.10% of the total fatty acid . The poly-unsaturated fatty acid is 56.07% of the total fatty acid in the mycelia .higher than Rivinosa and peanut . The fruitbody of L.deliciosus has 17 kinds of amino acids, the total content of which in pileus and stipe is respectively 12.75% and 8.98%. The essential amino acid makes up 48.39% of the total amino acids.The first limit amino acid is met.

松乳菇子实体中含有17种氨基酸,氨基酸的总含量在菌盖和菌柄中分别为:12.75%,8.98%,其中必需氨基酸含量占总氨基酸含量的48.39%,明显高于FAO/WHO模式的标准蛋白,略低于松口蘑和牛舌菌;松乳菇的第一限制氨基酸为蛋氨酸;化学评分、氨基酸评分、必需氨基酸指数、生物价、营养指数、氨基酸比值系数分分别为:77.6、64.9、98.6、95.8、23.2、74.19。

Eggs and soyabean milk contains soap protein enzyme stayer,which can control liveness of flesh's protein enzyme,affecting protein digestion and absorption internal to the body.Egg white contains tough protein,which can integrate with soap protein enzyme in the soybean milk,blocking the break down of protein,thereby reducing the protein absorptivity of the body.

鸡蛋与豆浆生豆浆中含有胰蛋白酶抑制物,它能抑制人体蛋白酶的活性,影响蛋白质在人体内的消化和吸收,鸡蛋的蛋清里含有粘性蛋白,可以同豆浆中的胰蛋白酶结合,使蛋白质的分解受到阻碍,从而降低人体对蛋白质的吸收率。

During the postnatal stage, whether PV and KCC2 or NKCC1 colocalized in the Vme and whether the percentage of colocalization varied is not clear and whether the variation coincided with the forementioned results is also not clear.

但是,NKCC1、KCC2与PV在Vme的生后发育过程中是否有共存,其共存的比例是否有变化,而这种相对量上的变化与蛋白表达水平改变的实际情况是否相符也无明确的答案。

Taking probiotic beverages as the carrier, related experiments were done on the following steps: screening out the best source collagen , figuring out the best working ratio of stabilizer in mixed solution, and confirming the effect on taste when titrating acid in finished products.

以益生菌饮料为载体,对胶原蛋白原料的筛选、稳定剂的复配比例、成品滴定酸度对口感的影响等进行了试验,确定了最佳工艺操作要点、产品配方及质量标准。

Interferon is a kind of high activity and multi function glucoprotein produced by interferon induced biological cells.

干扰素的基本特性干扰素(interferon,IFN)是在特定的诱生剂作用下,由细胞产生的一种具有高度生物学活性的糖蛋白。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

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我比喻得过头了。