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3PH was done in group M and T. Then the rats in B group and M group were injected normal saline via portal vein, and those in T group were injected with SHCs suspension. Four week later, the level of serous alanine aminotransferaseand glutamic oxalacetic transaminase were tested, and liver pathological changes observed. Frozen sections of T group were observed under fluorescent microscope.

将实验用同一近交系SD大鼠30只随机平均分成空白对照组、损伤造模组和SHCs移植组。B组腹腔注射生理盐水,M组、T组腹腔注射CCl4.24 h后戊巴比妥腹腔麻醉,消毒开腹,M、T组行2/3肝切,B、M组门静脉注入生理盐水,T组门静脉注入经膜荧光标记SHCs悬液。4周后,采血测血清谷丙转氨酶、谷草转氨酶,并取病理标本做冰冻切片荧光显微镜下观察及HE染色普通光镜下观察。

METHODS: An inflatable balloon was inserted between the periorbita and orbital floor in 5 fresh human cadavers. Hertel measurements were taken for each millimeter over a total 7ml volume increment. Eighty data points were achieved and analyzed statistically using SAS6.12 software package.

选择新鲜尸体5具共10侧眼眶,将气管插管的气囊部分置入眶底与眶骨膜之间,用注射器注入7ml生理盐水,每抽出1ml生理盐水后,用Hertel眼球突度测量计测量眼球突度,共得到80个数据,采用SAS6.12软件包进行统计学分析。

Forty rats were randomly divided into ALR therapy group and saline control group, which were injected peritoneally ALR gene (200μg/kg) and saline per 12h respectively 4h after CCl4 injection.

在染毒后4h,将大鼠随机分为治疗组和生理盐水组,每组20只,每12h分别腹腔注射ALR基因(200 μg/kg)和生理盐水,存活超过96h计为存活。

In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks.

在动物体内实验中,选择清洁级ICR纯系小鼠100只,按体质量随机分为5组:阴性对照组每天腹腔注射生理盐水,其余4组每天腹腔注射120 mg/kg D-半乳糖以建立小鼠衰老模型,同时阳性对照组每日以生理盐水灌胃,L、M、H组分别以每日100、200、400 mg/kg TP灌胃。8周后处死小鼠,获取心肌标本进行组织学研究和生化检测。

METHODS: The 316L stainless steel was dip into a mixture of 5 g/L quaternionic copolymer tetrahydrofuran and 10% RNAIII inhibiting peptide to obtain stable polymer coating, which was extracted at density of 1 cm2/mL in saline at 37 ℃ for 72 hours to get 100% eluent. The same volume of saline was added to make 50% eluent.

将洁净的316L不锈钢片浸渍于5 g/L的四元共聚物四氢呋喃和质量分数10%的RNAⅢ抑制肽混合溶液中,获得稳定的聚合物涂层,按1 cm2/mL在37 ℃无菌条件下用生理盐水浸提72 h,制得浸提液原液;加入同体积的无菌生理盐水制得50%浸提液。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明,处理后4周,全周角膜缘上皮板层手术切除,中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化,未发生睑球粘连,角膜基质胶原纤维完整未见溃疡、穿孔等病变,细胞印迹学检查为结膜表型,可作为实验性角膜缘干细胞移植的病理模型;全周角膜缘上皮板层手术切除,中央角膜上皮用生理盐水擦除的5只试验家兔,有2只为结膜表型,另3只为角膜表型,观察期内结果不稳定;半周角膜缘上皮板层手术切除,中央角膜上皮层用生理盐水擦除的5只试验家兔,角膜表面透明,全部为角膜表型;直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔,有4只角膜基质胶原纤维断裂、溶解,并伴有严重的溃疡、穿孔、睑球粘连等病变,不能用于移植试验,另1只角膜表面透明,未见结膜和新生血管长入,细胞印迹学检查为角膜表型。

Then killed the animals and extracted hippocampus.The serum of TChE,SOD CAT and MDA were detected.

然后将各组剩余小鼠断髓处死,冰台上迅速取出脑组织,在预冷的生理盐水中漂洗,除去血液,分离出两侧的海马,投入4℃冰浴的玻璃匀浆器中,按1∶9于生理盐水中制成10%脑组织匀浆,在4℃下4000r/min离心10min,取上清液以检测小鼠海马组织中乙酰胆碱酯酶、超氧化物歧化酶、过氧化氢酶的活力和丙二醛的含量。

Methods Eighty-eight patients with parcel tuberculous pleural effusion were randomized into two groups. The trial group used urokinase (100, 000 U) plus DXM (10mg) and NS (20ml) per time in the thoracic cavity after each thoracocentesis, and the control group used DXM (10mg) plus NS (20ml).

88例结核性包裹性胸膜炎随机分成治疗组与对照组,治疗组每次抽液后注人尿激酶10万单位+地塞米松10mg+生理盐水20ml,对照组抽液后,注入地塞米松10mg+生理盐水20ml,其他治疗相同。

Methods Thirty-six Wistar rats were randomly divided into four groups: group Ⅰ received intraperitoneal injection of L-thyroxine for 42 days to establish a model of thyrotoxic myopathy; group Ⅱ was treated with XYQJT for 30 days after L-thyroxine intraperitoneal injection; group Ⅲ was given normal saline for 30 days after L-thyroxine intraperitoneal injection as control; group IV served as normal control group.

方法36只健康雌性Wistar大鼠随机分为4组:甲亢肌病模型组10只,腹腔注射甲状腺激素钠盐每天1次,共42d;中药治疗组10只,腹腔注射甲状腺激素钠盐42d后,灌服消瘿强肌汤30d;生理盐水对照组10只,腹腔注射甲状腺激素钠盐42d后,灌服生理盐水30d。

Methods 70urethroplasty cases were randomly divided into2groups,the urethral stents were douched with0.9%N.S in the cotrol group,and the integrated nursing interventionwas applied in the treatment group,the INI includerays the use of10ml0.9%N.S plus80000units gentamicin for douche,swabbing genitals with nitrofurazone solution and infrared rays exposure of genitals.

选择尿道下裂带蒂皮瓣正位开口一期尿道成形术患者70例随机分为2组:一组是常规用生理盐水冲洗尿道支架管方法;另一组是生理盐水10ml加庆大霉素8万U冲洗新尿道支架管,呋喃西林溶液擦拭外阴部以及红外线伤口局部照射的护理干预方法综合护理,观察尿瘘的发生率。

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