生物鉴定

The polysaccharide fraction AGP-3 was obtained and analyzed preliminarily after water-soluble polysaccharides from Ampelopsis grossedentata being isolated and purified.

目的对藤茶水溶性多糖进行分离纯化得到AGP-3组分，并对其结构进行初步的鉴定，为藤茶多糖结构与生物活性关系的研究奠定基础。

In this paper, 13 lakes and 2 large-scale reservoirs in Wuhan were investigated, the makeup of species , dominance species and standing biomass of plankton, benthon, hydrophyte, fish and et al were carried out. And the bio-indicate method , diversity index are used to evaluate the water body.

本文对武汉市13个湖泊及2个大型水库浮游生物、底栖动物、大型水生植物、鱼类及其它生物的种类组成、优势种的鉴定及现存量的测定结果进行了分析，运用指示生物法、种多样性指数法对所调查水体进行了水质评价。

The authers give a brief account of the present situation and prospects for the application of immunochemical methods to agricultural sciences, inculding identification of species, diagnosis of animal and plant diseases, quantitative and locative analysis of substances of biological activity in living things, determinat...

本文介绍了农业科学研究中，在物种鉴定、快速诊断和检疫、生物活性物质的测定、性状标记物和次生代谢物的测定、食品和环境中残毒的检测、生物物质的定位等方面血清学技术的应用现状和前景。

The low temperature biofilm was composed of physchrotrophs, Through the experiments of isolation and identification, 8 strains of physchrotrophs were obtained from biofilm, they are Comamonas、Flavobacterium、Arthrobacter、Bacillus、Micrococcus、Pseudomonas、Achromobacter and Vibrio.

低温生物膜的活性主要来自其上面的低温微生物，分离鉴定试验结果表明，组成低温生物膜的微生物主要有8种，发现它们分别属于丛毛单胞菌属、黄杆菌属、节杆菌属、芽孢杆菌属、微球菌属、假单胞杆菌属、无色菌属和弧菌属。

The characters and performance of the biofilm were studied. When the concentration of nitrite is 206.82 mg/L, the velocity of nitrification is 260 mg/L·d. The best performed bacteria, N-20, were identified as Nitrobacter sp.

实验得到了稳定的自养硝化生物膜；当NO-2浓度为206.82mg/L时，生物膜的稳定硝化速率可达260mg/L·d；经鉴定，硝化速率最高的N20菌株属硝化杆菌属(Nitrobactersp。)。

Objective: To study the species, constitution and origination of bacteria in periapical biofilm.

目的：对根尖生物膜细菌进行分离、培养和鉴定，了解根尖生物膜细菌的种类、组成和来源。

Seedbox Screening Technique was applied to evaluate the reactions of two parents and 98 BILs to BPH at the seedling stage, and the entries were graded on ratio of dead seedlings.

采用褐飞虱生物1型和生物2型的混合种群进行苗期集团鉴定，并以死苗率作为抗褐飞虱表型值，分析亲本和98个BILs的抗褐飞虱表现。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

The identification experiment of biotype and resistance to the aphid on the wheat of Amigo was designed on the basis of differentiation characteristic of the aphid.

根据麦二叉蚜种下生物型分化特性，设计了小麦品种 Amigo的抗蚜性和麦二叉蚜生物型鉴定试验。

The journal has provided authoritative summaries of significant novel developments in this constantly evolving field for analytical chemists, environmental engineers, biochemists, biotechnologists and biologists since 1971, including critical evaluations of innovative methods, apparatus, chromatography, crystallization, extraction, distillation, ultrafiltration, magnetic separation, sedimentation and electrodialysis etc.

该杂志自1971年开始就为分析化学家，环境工程师，生物化学家，生物技术学家和生物学家提供了在这不断进化领域重要新进展的权威性摘要，包括对于创新方法的鉴定评价，器械设备，色谱，结晶，萃取法，蒸馏法，超滤作用，磁选，沉降以及电渗析等等。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。