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生物芯片

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So, it is a hotspot that how to effectively recognize the spot on the biochip .

如何有效地识别芯片上的斑点样本也成为生物芯片技术中的一个热点问题。

The detection instrument is an important component of biochip technology.

生物芯片探测技术是生物芯片技术中的重要组成部分。

The stability and accuracy of the biochip testing can be ensured and provided testing efficiency.

提高了生物芯片的检测效率,确保了生物芯片检测的稳定性和准确性。

Besides, because of diversity of biochip image, the shape of some spots can not be uniformly circular. Solved the problem of coarse segmentation and modification of deflexion angle, a method of tracking local spots profile—Template snake model has been proposed in the paper, it can recognize the target from roughness to precision step by step, so to guarantee the precision and efficiency of segmentation.

此外,由于生物芯片多样性特点,某些芯片样点外形并不一定是简单的孔径问题,本文在比较彻底地解决了样点粗分割及偏转角校正后,提出了局部区域的样点轮廓跟踪方法——Template snake模型,实现由粗到精的宏状多目标分层识别策略,既保证了样点分割的正确率,又加快了执行速度。

Results and Conclusions: As the recently developed international bio-visual micro-analysis method, QDs technology is the development and extension of this method. QDs technology has advantages in bio-labling, active cell and corporeal imaging, medical research, bio-chip fluorescent labels and screen.

结果与结论: QDs技术是近年来国际生物可视化微分析方法为代表的可视化分析微技术的发展和延伸,它在生物标记、活细胞和体内成像、药物研究、生物芯片的荧光标记与筛选研究等方面具有独特优势。

The CCD chip and image intensifier were cooled to increase the sensitivity of the system.

利用光锥耦合的ICCD系统探测荧光染料标记的生物芯片,并对CCD芯片和像增强器制冷,以提高探测灵敏度。

The visualization biological chip in this invention can make the sample simultaneously accept the high efficiency laser source and uses CCD cameral select the fluorescence image of the biological chip rapidly with large area for further analysis.

本发明的显像式生物芯片仪利用准直透镜使镭射光源产生大面积的镭射光激发光源,使得各样品点同时接受高效能的激发光源,此外利用CCD相机大面积而快速的选取生物芯片的荧光影像再进行分析,可以大幅减少分析时间。

Methods] The diagnosis model was established by SELDI technique with SAX2 chip to detect the serum of patients with gastric adenocarcinoma, gastricism and healthy persons, and tested by single-blind model.

]采用蛋白质生物芯片表面增强激光解析电离飞行时间质谱技术,运用SAX2蛋白质芯片检测胃腺癌患者、胃炎患者和健康者血清,建立诊断模型,然后进行单盲模型验证。

To acquire more information about gene expression at the whole-genome scale,our lab and National Engineering Center for Beijing Biochip Technology worked together and constructed the first genome-wide oligonucleotide microarray of the silkworm.This microarray was used to detect gene expression of 10 tissues/organs,including midgut,integument,head,hemocyte,testis,ovary,anterior/median silk gland,posterior silk gland,fat body and malpighian tubule.In this study,we performed analysis of these microarray data and confirmed the reliability of the results.

为了在全基因组水平上获得基因表达信息,本实验室与生物芯片北京国家工程中心合作,共同定制完成了世界上第一张家蚕全基因组寡核苷酸基因芯片,并利用该芯片对家蚕5龄第3天幼虫的中肠、体壁、头、血液、精巢、卵巢、前中部丝腺、后部丝腺、脂肪体、马氏管这10个组织中的基因表达谱进行了检测。

In the second chapter, p-hydrazino-benzenesulfonic acid was prepared by diazotizing sulfanili acid with sodium nitrite. The diazanium salt was then reduced with sodium hydrogen sulfite and acidified with sulfuric acid to give 83.4% product. After several repeated experiments, the reactant and reaction condition are determined.

由于Cy系列试剂的特殊结构使其在荧光标记方面与其他试剂相比有着显著的优点,在生物芯片和蛋白质组学方面得到了广泛的应用,因此文章综述了Cy系列试剂在DNA芯片和蛋白质组学研究中的标记方式以及应用。

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