生物素

Biotin is not saturated fatty acids required for the synthesis of the coenzyme acetyl CoA.

生物素是不饱和脂肪酸合成过程中所需的乙酰CoA的辅酶。

Gonorrhoeae was amplified with the one set of primer for the 16S rRNA gene. The reverse primers were labeled with biotin. The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a nylon membrane.

选择NG 16S rRNA基因设计一对PCR引物，生物素标记下游引物扩增NG DNA，然后与固定在尼龙膜上的特异性寡核苷核探针杂交。

The chromosome composition of Yuan Zhong 2, a common wheat-Ag.intermedium partial amphiploid (2n=54), was analyzed with the genomic in situ hybridization,in which the biotinylated total DNA of Ag.intermedium as the probe and the total DNA of common wheat cv.Chinese Spring as blocking DNA were used to hybridize with the chromosome DNA of Yuan Zhong 2 at mitotic metaphase in root tip cells.

为分析普通小麦-天兰冰草部分双二倍体-远中2号(2n=54)的染色体构成，用生物素(biotin-16-dUTP)标记天兰冰草染色体组DNA作为探针，以普通小麦品种中国春染色体组DNA为封闭DNA，与远中2号的有丝分裂中期染色体DNA进行了分子原位杂交。

Biotin is a kind of B-complex vitamins.

生物素是一种B族维生素。

Vitamins A, D, E, and E are all fat-soluble while B-complex vitamins, vitamin C and the bioflavonoids water-soluble.

维生素A、D、E和K为脂溶性维生素，B族维生素及维生素C和生物素属于水溶维生素。

It is involved in the synthesis of biotin.

它和生物素的合成有关。

In this thesis, we did modifications of alkyl groups upon benzyl fluorophosphonate. In order to study the influence of alkyl length and steric effect on activity and selectivity of probes, we synthesized benzyl fluorophosphonate recognition heads with modifications including ethyl, butyl, octyl and cyclohexyl. By way of connecting different kinds of tag like azido group, biotin and rhodamine with recognition heads separately through a higher hydrophilic ethylene glycol derived linker, a series of benzyl fluorophosphonate probes were made up. And they will apply to various biological testing strategies.

在本论文中，我们以苯甲基氟化磷酸酯为基础，於其上进行一系列烷基之修饰，为了探讨烷基长度与空间立体性质对於探针活性及选择性的影响，我们合成了包含乙烷基、丁烷基、辛烷基以及环己烷基修饰的苯甲基氟化磷酸酯辨识端，并藉由具较高亲水性的聚乙二醇衍生连接桥结合三种不同的发报端，分别有叠氮基团、生物素及萤光基团来构成不同的化学探针组合，这一系列苯甲基氟化磷酸酯类化学探针将可被应用於不同的生物测试策略。

Based on a comparative study of two coating methods, with mycoplasm hyopneumoniae or its antibody, a highly sensitive and accurate enzyme linked immunosorbent assay method has been developed for direct determination of biotin in the transformed yeast cultural media.

通过对用猪肺炎霉浆菌包被或用其兔抗体包被两种方法的比较研究，得到一种高灵敏度和高准确度对生物样品中生物素含量直接定量的酶联免疫吸附分析法。

With this SPR sensor, avidin of concentration of 0.5 nmol/L was examined and a nice response characteristic was obtained. The result indicates that this device could be widely employed for measuring biomolecules.

利用这种传感器，测量浓度为 0.55nmol/L的抗生物素，得到很好的响应特性，证明该装置可用于生物分子的检测。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。