生物素

Objective GFP-streptavidin bi-functional fusion protein was generated and characterized in order to demonstrate our novel platform for efficiently and durably modifying the cell surface with SA-tagged bi-functional proteins.

目的 GFP-SA双功能融合蛋白的制备及其鉴定研究，以展示我们建立的技术平台，即用含链亲和素的双功能融合蛋白对生物素化的细胞表面进行高效的锚定修饰。

AbstractSerum prostate specific antigen-the biotin-avidin enzym immunoassaywas estab-lished and used in clinical practice.

作者建立了血清前列腺特异抗原的生物素－亲和素酶联免疫测定法并用于临床。

The expression changes of ICAM-1 and p65 mRNA level were detected by reverse transcription-polymerase chain reaction and the expression of p65 protein in AR42J cell lines were detected with Stept Avidin-Biotin complex respectively, and the changes of amylase in culture medium with chromatometry.

逆转录－多聚酶链反应半定量法观察细胞间黏附分子(ICAM-1)和p65亚单位mRNA表达的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达；人工碘比色法观察培养液上清中淀粉酶的活性改变。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

Methods Streptavidin-biotin complex immunohistochemistry was used to explore the expression of CD44V6 and PCNA in 35 cases of ocular squamous cell carcinomas, 20 cases of papillomas, and 11 cases of normal eyelid tissue.

方法应用链霉亲和素-生物素-过氧化物酶复合物免疫组化法检测35例眼部鳞状细胞癌、20例乳头状瘤和11例正常眼睑皮肤组织标本CD44V6蛋白表达。

The affinity constant of E1-McAb reacting with coated complete antigen was 8.2×10^8L/mol. Biotin-streptavidin complex was used for the determination of E1. The calibration curve of free E1 covered a range of 10～10 000 pg/mL.

以生物素化的羊抗鼠免疫球蛋白及辣根过氧化物酶标记的链亲和素为标记体系，通过竞争抑制的方式测定游离的雌酮，结果表明：雌酮在10～10000pg/mL内呈线性关系。

Avidin is a glycoprotein, molecular weight 60,000, each molecule consists of four energy and biotinylated subunits.

亲和素是一种糖蛋白，分子量60000，每个分子由4个能和生物素结合的亚基组成。

We delivered avidin to the biotin/SiNW-FET surface by injecting an avidin sample into a microfluidic channel system.

在流体实验中，通道的质量传递效应对卵白素与生物素的结合行为有很大的影响。

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素－链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人，测定血浆CT-1水平和血浆sICAM-1水平。

ABC technique was employed in the study by marking a single specific antigen with horse radish peroxidase to determine the IgG in the injured human skin tissue both before and after death.

卵白素—生物素过氧化物酶复合物技术，直接用辣根过氧化物酶标记单一特异性抗体检测人体生前与死后损伤皮肤组织中的 Ig G。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。