生物素

Methods: Using biotin labelled ECL, UEA and PHA-L as probes, we examined the distribution and dynamic changes of their receptors with affinity cytochemistry and image analysis.

以生物素标记的凝集素ECL、UEA和PHA-L为探针，应用亲和细胞化学和图像分析的方法，检测上述3种凝集素相应受体的分布状况和变化规律。

Methods: Using biotin labeled ECL,UEA and PHA-L as probes,the distribution and dynamic changes of their receptors we reexamined by affinity cytochemistry and image analysis.

以生物素标记的凝集素ECL、UEA和PHA-L为探针，应用亲和细胞化学和图像分析的方法，检测上述3种凝集素相应受体的分布状况和变化规律。

Methods: Using biotin labeled ECL, UEA and PHAL as probes, the distribution and dynamic changes of their receptors we reexamined by affinity cytochemistry and image analysis.

以生物素标记的凝集素ECL、UEA和PHAL为探针，应用亲和细胞化学和图像分析的方法，检测上述3种凝集素相应受体的分布状况和变化规律。

Biotinylated lectins:UEA-Ⅰ, RCA-Ⅰ, DBA, PSA, PNA, BSL, LCA, WGA, ConA AND SBA were used as probes to identify lectin binding sites and to determine if lectin binding patterns change with age in the developing of human fetal esopha geal epithelium (8W, 14W, 20W, 28W and 32W).

采用十种生物素化凝集素UEA-I、DBA、PSA、B SL、PNA、LCA、RCA-I、SBA、ConA及WGA分别对8W、14W、20W 28W及32W的人胎儿食管上皮进行研究，以确定胎儿食管上皮在发育过程中凝集素的结合位点以及结合方式随年龄的变化。

Methods Both in malformed embryos induced by excess retinoid acid and normal KM mice, expressions of lectin receptors of WGA, RCA and ECL in the epithelium of palatal shelves and the mesenchyma were determined by affinity histochemistry using biotin-marked corresponding lectins.

以生物素标记的凝集素受体WGA、RCA和ECL为探针，应用亲和细胞组织化学染色方法检测上述3种凝集素受体在RA致畸鼠胚和相应对照组鼠胚腭板上皮及其间充质的分布状况和变化规律。

The dynamic distribution of three different glycoconjugates in oocytes and follicle cells during the oogenesis of Atractomorpha sinensis were detected using biotin-labeled Peanut Agglutinin, Soy Bean Agglutinin and Ulex Europaeus Agglutinin I lectins.

以生物素标记的凝集素(UEA-I、 SBA、 PNA)为探针，利用凝集素组织化学方法对短额负蝗卵子发生过程中滤泡细胞和卵母细胞内糖复合物的分布进行了定位研究。

Methods Avidin biotin-peroxidase complex technique was used to determine mCD14 protein expression in peripheral monocytes in 5 ARDS patients and 10 normal subjects.

对5 例ARDS患者及10 例健康志愿者，分别分离其外周血单核细胞，用抗生物素－生物素－过氧化酶复合物技术检测其mCD14 的表达。

An abundance of avidin in the duct can result in a deficiency of biotin.

活性'。''减低。导管中''。'抗'。''生物素蛋白过量可导至生物素的缺乏

Its principle is as follows:red blood cells are labeled with NHS-Biotin,participated in the circulation and ages in vivo.Using the high affinity between biotin and avidin,the labeled RBCs with specific ages are separated.

其基本原理是用生物素标记一定量的红细胞，使之参与血液循环，并在体衰老，利用biotin与抗生物素蛋白之间的高亲和力，分离带有标记的、具有特定年龄的红细胞。

The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。