生物素

They placed genes from the wormwood plant into a yeast organism and got it to produce large amounts of artemisinic acid.

他们把青蒿的基因放置到一种酵母的生物体内，并让它生产出大量的青蒿素酸。

Keywords Biomarker;Metallothioneins;Resonance light scattering;Alizarin red;Xylenol orange;mercury;Light green SF;cadmium;Graphite Furnace atomic absorption spetrophotometry;environmental risk asse

Article From 南华大学；卫生毒理学硕士论文 2007年度生物标志物；金属硫蛋白；共振光散射；茜素红；二甲酚橙；汞；亮绿SF淡黄；镉；石墨炉原子吸收分光光度法；环境风险评价

Shandong Shengli Bioengineering Co., Ltd., Jining 272037, Shandong, China Abstract: The cause of infection in the process of avermectin production was studied from infected time, scope and bacterial type. The test method to check the infection caused by other bacteria was also raised. The ways to prevent infection was ascertained from preparation of microbial strain, air purification, medium disinfection, ductwork deployment and systematic dead angle cleaning. Meanwhile, adjustment measure was summarized in different infected period such as microbial strain, seed pot and yeastiness.

山东胜利生物工程有限公司，山东济宁 272073 摘要：从染菌的时间、类型、范围三方面探讨了阿维菌素发酵染菌的原因，提出了发酵过程中杂菌的检查方法，分别从菌种制备、空气净化、培养基灭菌、管路配置、系统死角清理等方面明确了防止发酵染菌的措施，得出了斜面菌种、种子罐、发酵罐不同时期染菌后的补救措施。

The acute toxicities of four representative non-target organisms: bee, partridge, zebra fish and silkworm, caused by emamectin benzoate were evaluated.

测定了甲氨基阿维菌素苯甲酸盐对4种有代表性的非靶生物蜜蜂、鹌鹑、斑马鱼、家蚕的急性毒性，并进行了安全性评价。

Xylostella. The synergism ability with dipping method l4as more obvious than with contact and stomach method. Among the six adjuvants, copper abietate, 1427 and 412103 had 3.71, 2.82 and 2.72-fold synergism ratio, respectively.

结果表明：在400mg/L以下，各阳离子助剂单独使用对小菜蛾3龄幼虫均无生物活性，但可明显提高阿维菌素对小菜蛾的药效。

Fullerene is the third allotropic form of carbon besides diamond and graphite, C60 is the representative in the fullerenes.

富勒烯是继石墨，金刚石后出现的碳的第三种同素异形体，其中C60是最典型的一种，由于其独特的结构和性能，使得它在生物，化学，材料，纳米科学等领域显示了巨大的应用潜力。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

In order to study functions of glutathione S-transferase theta during preparation and synthesis of fibroin in Antheraea pernyi, expression of GSTT in silk glands of the fifth instar larvae was analyzed.

为明确柞蚕谷胱甘肽硫转移酶－theta在柞蚕丝素蛋白准备和合成过程中所起的作用，采用生物信息学的方法克隆了柞蚕GSTT基因，并分析其在5龄幼虫丝腺中的表达规律。

It contains rich ecological amino acids extracted from bathypelagic organism, MG-60 moisturizing essence of natural fucose, and natural cleanser extracted from ecological pollution-free plants.

商品简介富含原生态深海生物提取的氨基酸、天然海藻糖MG-60保湿精华、原生态无污染植物萃取的天然清洁素，不仅能高效温和地清洁肌肤，而且革命性水能量储送技术能将保湿因子快速输送到角质层进行补水，并在皮肤表面形成一层水保护膜，在洁面时有效防止肌肤内部水份同表肌水一同蒸发，令肌肤柔滑滋润、无紧绷感。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。