生物素

Methods: Seventy-eight specimens of disc herniation(classified as protrusions,, extrusions, and sequestrations) and fourteen nonherniated discs were immunostained with antihuman MMP-3 monoclonal antibody, using the avidin-biotin-peroxidase complex method.

运用免疫组织化学抗生物素蛋白—生物素—过氧化物酶复合体法，通过抗MMP3的单克隆抗体，对78例椎间盘突出症和14例非椎间盘突出症的椎间盘标本进行染色。

Health and egg contain harmful bacteria and viruses, in particular, contains egg albumin is an anti-biotin factors, would prevent the use of biotin absorption04-12-11

健康和鸡蛋含有有害的细菌和病毒，尤其是，含有卵清蛋白是一种抗生物素因子，将防止使用生物素的吸收

Endogenous biotin is expressed mostly in the fundic endoplasm of surface epithelial cells in colonic tissue samples from human beings.

内源性生物素在结肠组织中主要表达在表面上皮细胞胞质，尤其是近基底侧；免疫组化时可首选生物素标记的检测系统。

RESULTS: EB was expressed on the fundic endoplasm of surface epithelial cells in 8 of the 14 colonic tissue samples, and 15% egg white blocking fluid could eliminate EB in colonic tissues from human beings.

结果： 8例出现内源性生物素阳性表达(57.14%)，主要位于表面上皮细胞胞质，尤其近基底侧。 15%蛋清封闭液可以封闭结肠组织中的内源性生物素。

Immunohistochemical localization of inhibin α-subunit in normal mature rats was carried out by using Strept Avidin-Biotin-Peroxidase Complex .The results showed that there was positive immunoreactivity of the McAb to inhibinα-subunit in heart ,kidney , spleen , lymph ,adrenal gland and pancreas cells. It indicated that inhibinα-subunit existed in the above tissues , But no positive sites were seen in hypothalamus, pituitary, lung and liver, that means they have no inhibinα-subunit. Compared with the ovariectomized rats, the hypothalamus and pituitary of the rats injected with inhibin-α fragment（1～32）showed an positive reaction, suggesting that inhibin α-subunit could pass through the blood-brain barrier .

用链霉亲和素-生物素-过氧化物酶(Strept Avidin-Biotin-Peroxidase Complex， SABC)免疫组织化学方法，以抑制素α亚基单克隆抗体对正常雌性性成熟大鼠各组织的抑制素α亚基进行定位观察，结果显示：⑴大鼠心脏、肾、脾、淋巴、肾上腺、胰腺均呈阳性反应，表明存在抑制素α亚基；下丘脑、垂体、肝脏和肺组织呈阴性反应，表明其不存在抑制素α亚基；⑵与去卵巢大鼠相比，外源注射抑制素α（1～32）片段的大鼠其下丘脑和垂体出现阳性反应，说明抑制素α亚基能通过血脑屏障。

The former will affect digestion and absorption of protein; the latter with the food combining biotin, leading to a lack of human biotin, mental fatigue, myalgia and other symptoms.

前者会影响蛋白质的消化吸收；后者能与食物中的生物素结合，导致人体生物素缺乏，产生精神倦怠、肌肉酸痛等症状。

Immunohistochemical staining indicated that Nattokinase positive staining grains were found in the brush-border membrane of epithelial cell from duodenum, jejunal to ileal in the Nattokinase extract group, and they showed most predominant in the jejunum, and there were many nut-brown positive staining grains in kytoplasm of epithelial cell.

利用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物(Streptavidin-biotin-peroxidase complex，SABC)免疫组织化学技术，在国内外，首次对兔口服摄入的纳豆激酶进行肠道吸收定位研究。

Objective:By histochemical method, the four biotinylated lectins (DBA, SBA, WGA, UEA 1) were used to study changos in glycoconjugate expression on the surface of endometrium of 28 cases of RSA without obvious reasons in secretary phase, at the same time, endometrial biopsies were taken for histomorphologic evolution.

本文通过生物素分别标记的双花藕豆凝集素、大豆凝集素、麦胚凝集素及荆豆凝集素（UEA－1）与子宫内膜的反应，应用S－P法研究28例无明显原因反复流产的分泌期子宫内膜糖复合物的变化及与其组织形态学分期的相关性。

Advantages of specific coupling between biotin and avidin or streptavidin allow firefly luciferase to be immobilized on solid support coated with avidin or streptavidin, so that more flexible and convenient methods of luminescence assay will be available in application.

利用生物素与配体亲和素或链霉亲和素的特异性耦合，可以将荧光素酶固定到亲和素或链霉亲和素包被的磁珠上，从而使荧光检测的应用更加灵活和方便。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。