生物素

Chemical senses; chemoreception; chemoreceptors; infochemicals; semiochemicals; chemical stimuli; chemical communication; chemical cues; chemical perception; pheromones; pheromone receptors; pheromone biosynthesis-activating neuropeptide; PBAN; pheromone-binding protein; pheromone glands

化学感官；化学感受；化学接受体；信息化学；化学信息素；化学刺激物；化学通讯；化学线索；化学感知；信息素；信息素受体；信息素生物合成激活肽；PBAN；信息素结合蛋白；信息素腺

Chemical senses; chemoreception; chemoreceptors; infochemicals; semiochemicals; chemical stimuli; chemical communication; chemical cues; chemical perception; pheromones; pheromone receptors; pheromone traps; pheromone emission; pheromone biosynthesis-activating neuropeptide; PBAN; pheromone-binding protein; pheromone glands; Dufour's gland; scent gland; scents; scent marking behavior; territorial behavior; trail following behavior; trail pheromone; aggregation pheromone; alarm pheromone; marking pheromone; oviposition deterring; queen pheromone; sex pheromone

化学感官；化学感受；化学接受体；信息化学；化学信息素；化学刺激物；化学通讯；化学线索；化学感知；信息素；信息素受体；信息素诱骗；信息素发射；信息素生物合成激活肽；PBAN；信息素结合蛋白；信息素腺；杜氏腺；臭腺；气味；臭迹行为；区域性行为；踪迹跟随行为；追踪信息素；群集信息素；报警信息素；标记信息素；产卵阻止；母蜂信息素；性信息素

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

English Outline Describes the classification and characteristics of biochemical reactions, major metabolic pathways; biochemical thermodynamics, biokinetics, immobilization technology, bioreactor construction, experimental design, mass transfer, heat transfer, and flow pattern; mathematical modeling, computer simulation, and optimization; the classification of reactors, aerobic and anaerobic, their operational characteristics and applications.

Chinese Outline 探讨生物化学反应种类及其特性，主要代谢路径；生物热力学、生物动力学、酵素特性探讨与酵素动力学，固定化技术、生物反应器之组装、实验设计、反应器内质传、热传及流动型态解析；数学模式，电脑模拟及最适化；简介反应器种类：好氧及厌氧反应器，其操作特性与应用。

Important advance was made to Celastrus and ceangulin V after 20 years studies, but the target of Celangulin V was not verified and the metabolic dynamic of Celangulin V in organism was not touched because of the technique difficulty in testing and analysis of trace Celangulin V in organism. It will solve the difficulty that establishing the immunoassay of Celangulin V. The immunoassay of Celangulin V including the synthesis of artificial antigen, the preparation of antiserum, the immunoanalysis location of Celangulin V and the quantitative immunoassay of Celangulin V were studied systematicly in this paper.

苦皮藤素V是从卫矛科植物苦皮藤中分离出的一种具有杀虫活性的天然产物，可能作用于一类新的作用靶标。20多年对苦皮藤及苦皮藤素V的研究已取得了重要进展，但迄今还没有证实苦皮藤素V的作用靶标，对苦皮藤素V在生物体内的药代动力学研究也尚未涉及，这些方面的研究因为对痕量苦皮藤素V在生物体内分析、检测存在技术方法上的困难而受到限制，建立苦皮藤素V的免疫分析技术则可解决这些问题。

Important advance was made to Celastrus and ceangulin V after 20 years studies, but the target of Celangulin V was not verified and the metabolic dynamic of Celangulin V in organism was not touched because of the technique difficulty in testing and analysis of trace Celangulin V in organism. It will solve the difficulty that establishing the immunoassay of Celangulin V. The immunoassay of Celangulin V including the synthesis of artificial antigen, the preparation of antiserum, the immunoanalysis location of Celangulin V and the quantitative immunoassay of C'elangulin V were studied systematicly in this paper.

苦皮藤素Ⅴ是从卫矛科植物苦皮藤中分离出的一种具有杀虫活性的天然产物，可能作用于一类新的作用靶标。20多年对苦皮藤及苦皮藤素Ⅴ的研究已取得了重要进展，但迄今还没有证实苦皮藤素Ⅴ的作用靶标，对苦皮藤素Ⅴ在生物体内的药代动力学研究也尚未涉及，这些方面的研究因为对痕量苦皮藤素Ⅴ在生物体内分析、检测存在技术方法上的困难而受到限制，建立苦皮藤素Ⅴ的免疫分析技术则可解决这些问题。

The biotechnology production efficiency of crocin II, which has anti-cancer activity, was improved in a solid-liquid two-step cell culture in which the callus cells obtained from the solid medium were transferred to the liquid medium.

为提高现代生物技术生产藏红花素的产率和效率，该研究采取固液两步培养法将在固体培养基中获得的藏红花愈伤组织转移到液体培养基中进行生物合成，结果显示生物反应器比摇瓶培养更有利于生物量的积累，也更有利于2号藏红花素的合成。

These ferments are made of the roofs, leaves and stems of 58 handpicked kinds of plant and vegetables through exclusive the biochemical technology, with the high-reactivity, high-stability and high-safety; The second is the fruit compound ferments(the approval No.J11000510836) with the abundant vitamin, minerals, favorable viable groups, multi-amino acid groups, oligosaccha groups and glucose , is made from handpicked 103 kinds of fruits and 20 kinds of Chinese herbal medicines through microbial treatment and fermentation method; The third is grain and bean high-fiber nutritious ferments (the approval No.J11000600369) with abundant proteases, amyloclast enzyme and lipolytic enzyme, is made from 60 kinds of grains and beans through special biologic technology, and is used to lose weight, expel toxin, clear the bowels, supply nutrition and activate cells in human body.

公司精选台湾58种植物、蔬菜的根、茎、叶天然原料，以独特的生化科技手段研制而成，不仅具有高活性、稳定性和安全性的特点，而且具有耐高温、耐强酸的特点，不含任何添加剂，其活性是当今世界任何厂家生产的酵素都不可比拟的。第二宝是水果复合酵素（检验审核号是J11000510836）。公司精选台湾103种水果，通过微生物科技处理，经半年多时间发酵酿制而成，含有丰富的维生素、矿物质、有益活菌群、氨基酸、果寡糖、葡萄糖等。第三宝是谷豆高纤营养酵素（检验审核号是J11000600369）。公司精选60种谷、豆及种子，用生物科技手段，经过特殊研制而成，含有丰富的蛋白质分解酵素、淀粉分解酵素及脂肪分解酵素。

Corn seedling were grown for 5 harvests in pot experiments to evaluate potassium supplycapacity of the soils.Results indicated that when soil avaliable potassium decreased,the dryplant matter was firstly affected,secondly was the total uptake of potassium by plant,then was thepotassium content in plant.Exchangeable,nonexchangeable and mineral potassium wereavaliable for plant,but the contribution of each potassium form was depended on soil types.

本文在研究褐土、潮土不同层次土壤的供钾特性、土壤各形态钾素的动力学行为以及小麦根系对钾素的吸收动力学特征的基础上，以玉米幼苗从土壤中的吸钾量为对照，考察了土壤钾素动力学特征与土壤钾素生物有效性的关系，运用热力动力学理论建立了土—根系统中的钾素能态链，并确立了土壤钾素生物有效性的能态指标。

It was susceptible to doxycycline, azithromycin, josamycin, especially the susceptibly of doxycycline reaches to 98.8%. Doxycycline can be considered as the first choice of antibiotic to treat biotype one of Ureaplasma urealyticum in the near future.

女性生殖道中Uu生物一群主要对喹诺酮类药物耐药，敏感药物主要为强力霉素，阿歧霉素及交沙霉素，尤其强力霉素的敏感性达到了98.8%，近期内可作为治疗Uu生物一群的一线用药。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。