生物素

We review micronutrient deficiencies for the major vitamins A, cobalamin B(12, biotin, vitamins C and E, as well as the minerals iron, and zinc, in the developed world, in terms of their relationship to systemic health and any resulting ocular disease and/or visual dysfunction.

本文分析了发达国家微量元素缺乏，包括维生素 A ，维生素 B12，生物素，维生素 C 和 E ，以及铁，锌缺乏与全身健康状态以及眼部疾病或视力损害的关系。

Main constituent:immunopotentiator, axerophthol, cholecalciferol, tocopherol, thiamine, vitamin b2, pyridoxine, cobalamin, niacin, pantothenic acid, folic acid, biotin, protein and so on.

主要成分：免疫增强剂、维生素A、维生素D3、维生素E、维生素B1、维生素B2、维生素B6、维生素B12、烟酸、泛酸、叶酸、生物素、蛋白质等。

Essential component: Micro biological element, deep sea fish roe essence, collogen, CM nanometer repair factor and so on.

主要成分：微量生物素、深海鱼子精华、胶原蛋白、CM纳米修复因子等。

The vitamins are divided into two general categorizes based on their solubility in either water or fat .the fat-soluble vitamins are vitamins A, D, E, and K; the water-soluble vitamins include vitamins C, niacin, thiamin, riboflavin, flacon antithetic acid, pyridoxine, vitamin B12 and biotin.

维生素按其可溶于水或脂肪分为两大类：脂溶性维生素，有维生素A、D、E、K；水溶性维生素，包括维生素C、烟酸、硫胺素、核黄素、叶酸、泛酸、吡哆醇、维生素B12和生物素。

The vitami are divided into two general categorizes based on their solubility in either water or fat .the fat-soluble vitami are vitami A, D, E, and K; the water-soluble vitami include vitami C, niacin, thiamin, riboflavin, flacon antithetic acid, pyridoxine, vitamin B12 and biotin.

维生素按其可溶于水或脂肪分为两大类：脂溶性维生素，有维生素A、D、E、K；水溶性维生素，包括维生素C、烟酸、硫胺素、核黄素、叶酸、泛酸、吡哆醇、维生素B12和生物素。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针，并将合成的特异探针固定在硝酸纤维素膜上，与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应，通过观察杂交信号判断存在何种细菌感染，同时研究该方法的敏感性和特异性。2。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测：利用已经克隆的美国柑桔裂皮类病毒cDNA序列，制备生物素标记的核苷酸探针，采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测，通过胶片曝光显示杂交信号，发现在两种方法的检测结果中阳性材料都有强的反应，而阴性材料无反应或较弱，该方法安全可靠、快速准确，适合大量样品的检测。

The genomic DNA of Thinopyron intermedium labelled with biotin-16-dUTP was hybridized with the chromosomal DNA of Wheat-Th. intermedium addition Line Z,(2n = 44), which had a good resistance to barley yellow dwarf vims.

以生物素（Biotin－16－dUTP）标记中间偃麦草基因组 DNA为探针，与抗黄矮病小麦－中间偃麦草染色体异附加系Z6进行原位杂交，鉴定出附加的1对中间偃麦草染色体。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Cox's proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、nm23和p53蛋白的表达情况。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Coxs proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、 nm23和p53蛋白的表达情况。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。