生物素

Objective To establish a sensitive and specific gene probe method for detection of Staphylococcal enterotoxin B gene.

目的 建立一种灵敏度高、特异性好的DNA 探针检测葡萄球菌肠毒素B 基因方法。方法用聚合酶链反应扩增的方法进行葡萄球菌B 型肠毒素基因探针生物素标记，用制备好的基因探针，进行斑点杂交，对葡萄球菌肠毒素10 株标准株和39 株临床株分别进行了检测。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetle s, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

Protein, soy fiber, xanthan gum, apple pectin, citrus pectin, blond psyliium seed powder, Algae, vitamins and minerals, vegetable powders broccoli, barley grass, wheat grass, spinach, beet green, cactus, collard, Jerusalem artichoke, golden seal, Echinacea angustifolia leaf, green pepper, wild yam, kale, rose hips, Lingzhi mushroom (Ganoderma lucidum, extracts of green tea and cola nut, American Sacred Bark, apple, prune and carrot juice, licorice.

蛋白质、大豆纤维、黄原胶、苹果胶、柑桔果胶、blond psyliium seed powder、绿藻、维生素A、B1、B2、B3、B5、B6、B12、C、D、E、叶酸、生物素，矿物质钙、镁、磷、铬、铜、碘、铁、锰、钼、硒、锌、氯、钾、矽，蔬菜粉(西芥兰、大麦草、小麦草、菠菜、甜菜)、仙人掌、芥兰、朝鲜蓟、金印草、紫锥菊、青椒、山药、羽衣甘蓝、玫瑰果、灵芝、绿茶、可乐果、American sacred bark、苹果、梅、胡萝卜汁、甘草。

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Raw egg white contains avid in which antagonizes biotin and thus the animal suffers from this vitamin deficiency .

生蛋清含有爱好者在对抗生物素，因此该动物患有维生素A缺乏症。

It was found that thenanoparticle-labeled SA prepared by this method remains high biological activityandstability for a long time.

这种方法不但使得纳米微粒和SA 稳定地连接在一起，而且保证了SA 在固相免疫分析中有很高的生物素亲和性。

DESIGN: Marginal biotin deficiency was induced in 7 adults (3 women) by egg-white feeding for 28 d. Blood and urine were obtained on days 0, 14, and 28 and 44 and 65.

设计：通过28天蛋白的食用来诱导7名（3名女性）生物素缺乏者，并在0、14和28天以及44和65天收集血样和尿样。

When applied the spiny eel X chromosome library probes to the chromosomes of the two fish, different signals were distributed: in Siniperca chuatsi, besides some little signals on lot of autosomes, a large bright signal region was detected on the 8th chromosomes, which represented a synteny of spiny eel X chromosomes; but in Channa argus, no distinct synteny was found.

当以刺鳅的生物素标记X文库探针对鳜鱼和乌鳢的有丝分裂染色体进行染色体涂绘后，经过核型分析和统计分析表明，在这两种鱼的染色体上的杂交信号分布是不同的：在乌鳢中，除散在信号外，在其第八号染色体近着丝粒部位存在着一段较大的信号结合区域，应是与刺鳅的X染色体同源的保守同线群，而在鳜鱼中，只有散在信号分布于多条染色体上，没有明显的保守同线群存在。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。