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Therefore, in the present study, a retrograde and anterograde experiments were made in the rat to address this issue. After injection of cholera toxin B subunit, a retrograde tracer, into Barrington's nucleus or D-region, some retrogradely labeled neurons were found in lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus of the lumbosacral spinal cord segments. Injection of biotinylated dextran amine into lamina I, sacral parasympathetic nucleus and dorsal commissural nucleus, respectively, a large number of BDA-labeled axon terminals were seen in Barrington's nucleus and D-region.

发现,将逆行示踪剂霍乱毒素 B 亚单位( Cholera toxin B subunit; CTb )注射到 Barrington's 核或 D 区后,在大鼠腰骶髓 I 层、骶髓副交感核和后连合核内分布有一定数量的 CTb 逆标神经元;将顺行示踪剂结合生物素的葡聚糖胺(Biotinylated dextran amine; BDA)分别注射到腰骶髓 I 层、骶髓副交感核和后连合核内后, Barrington's 核和 D 区内出现大量顺行标记的轴突终末。

Objective To explore the effects of biotin dextran amine as a nerve tracer in rat corticospinal tract.

目的 探讨生物素葡聚糖胺神经示踪剂在大鼠皮质脊髓束通路的神经示踪实验研究。

Methods Immunohistochemical streptavidin-peroxidase method was used to detect the expression of MMP-2, MMP-9, TIMP-1, TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles, 55 cases of complete hydatidiform moles, 15 cases of invasive moles and 8 cases of choriocarcinoma.

应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(2周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。

The barley chromosome in wheat was identified by genomic in situ hybridization in which biotin labelled total genomic DNA of barley betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring as blocking DNA. A series of wheat materials were tested as follows; two disomic alien substitution and monosomic alien addition lines, 2n=43; two monosomic alien substitution lines.

生物素(Biotin-16-dUTP)标记的大麦Betzes基因组DNA作探针,以普通小麦中国春总DNA作封阻进行基因组原位杂交(Genome in situ hybridiz ation,简称GISH),从13株小麦-大麦杂交后代中鉴定出2个含有3条大麦B etzes2H染色体的材料(2n=43);2个2H单体异代换系(2n=42);7个2H二体异代换系(2n=42)。

The pathway The first committed step in Fatty acid biosynthesis is the carboxylation of acetyl CoA to form malonyl CoA which is catalyzed by the biotin-containing enzyme acetyl CoA carboxylase.

途 径 脂肪酸合成的第一个关键步骤是乙酰-COA 羧基化形成丙二酰单酰 COA ,这一步是在含生物素的酶乙酰-COA 羧化酶的催化下进行的。

Ingredients: Apple Cider Vinegar, Magnesium Chelate, Monocalcium Phosphate, Inositol, Ascorbic Acid, Biotin, Mixed Tocopherols, Niacinamide, Calcium Pantothenate, Zinc Chelate, Manganese Chelate, Vitamin A Palmitate, Copper Chelate, Vitamin D, Selenium Chelate, Chromium Chelate, Pyridoxine HCl, Ribofl avin, Thiamine HCl, Molybdenum Chelate, Cyanocobalamin, Folic Acid, Vanadium Chelate, Potassium Iodide.

果醋,螯合镁,磷酸一钙,肌醇,抗坏血酸,生物素,混合生育酚,烟碱醯胺,泛酸钙,螯合锌,螯合锰,维生素A棕榄脂,螯合铜,维生素,螯合硒,螯合铬,盐酸?哆辛,核黄素,盐酸硫胺素,螯合钼,氰钴,叶酸,螯合钒,碘化钾

Mycoprotein is a source of protein, fibre, biotin, iron and zinc, and is low in saturated fat.

真菌蛋白是一种来源的蛋白质,纤维,生物素,铁和锌,是低饱和脂肪。

The distribution of γ-aminobutyric acid and serotonin (5-HT) immunoreactive neurons in the protocerebrum and deutocerebrum of the butterfly Minathyma schrenckii were studied with the Colophony-paraffin embedding serial section technique and the Streptavidin-peroxidase immunohistochemical method.

采用树脂石蜡(Colophony-Paraffin,CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase,SP)免疫组织化学方法,首次研究了 GABA 和5-HT 免疫阳性神经元在白斑迷蛱蝶前脑和中脑中的分布。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针,进行原位杂文,研究POMCmRNA在大鼠卵巢中的分布,实验表明,POMCmRNA主要分布于间期,妊娠和假孕大鼠卵巢黄体中,间质细胞也可见少量POMCmRNA,而卵泡颗粒细胞中POMCmRNA极少。

To monitor the effieciency of this site-directed serodiagnostic procedure, the synthetic peptides adhered to the microplates was biotinylated in situ with biotin of an extended spacer arm and avidin-biotinylated horseradish peroxidase complex was used.

为控制定位血清学检测质量,本研究应用长臂生物素对吸附于反应板上的合成肽作&原位&标记,经ABC结合和底物显色并计算吸附指数,首次为检测合成肽对反应板吸附性建立了一种简单可靠的方法。

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