生物素

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

Chicken Meal, Ground Yellow Corn, Ground Whole Wheat, Lamb Meal, Poultry Fat, Natural Flavors, Full - Fat Soybeans, Fish Meal, Dried Eggs, Dicalcium Phosphate, Salt, Dried Kelp, Choline Chloride, Brewer's Dried Yeast, Natural Antioxidants, Vitamin Supplements (E,A,B2,B12,D3), Niacin, Calcium Ascorbate, Calcium Pantothenate, Thiamine Mononitrate, Inositol, Menadione Sodium Bisulfite Complex, Folic Acid, Biotin, Zinc Proteinate

鸡肉，黄玉米，优质麦芽，羊肉粉，家禽脂肪，天然香料，大豆卵磷脂，鱼粉，蛋粉，磷酸氢钙，盐，干海带，氯化胆碱，啤酒酵母干燥，天然抗氧化剂，维生素补充剂（五，甲， b2的， b12的，维生素d3 ），烟碱酸，钙，抗坏血酸，泛酸钙，盐酸吡多辛，肌醇，维生素k3亚硫酸氢钠复合物，叶酸，生物素

Chicken Meal, Ground Yellow Corn, Ground Whole Wheat, Lamb Meal, Poultry Fat, Natural Flavors, Full - Fat Soybeans, Fish Meal, Dried Eggs, Dicalcium Phosphate, Salt, Dried Kelp, Choline Chloride, Brewer%27s Dried Yeast, Natural Antioxidants, Vitamin Supplements (E,A,B2,B12,D3), Niacin, Calcium Ascorbate, Calcium Pantothenate, Thiamine Mononitrate, Inositol, Menadione Sodium Bisulfite Complex, Folic Acid, Biotin, Zinc Proteinate

鸡肉，黄玉米，优质麦芽，羊肉粉，家禽脂肪，天然香料，大豆卵磷脂，鱼粉，蛋粉，磷酸氢钙，盐，干海带，氯化胆碱，啤酒酵母干燥，天然抗氧化剂，维生素补充剂（E，A， b2， b12，维生素d3 ），烟碱酸，钙，抗坏血酸，泛酸钙，盐酸吡多辛，肌醇，维生素k3亚硫酸氢钠复合物，叶酸，生物素

Chicken, Chicken Meal, Whole Ground Brown Rice, Whole Ground Barley, Chicken Liver, Pea Fiber, Chicken Fat (Preserved with Natural Mixed Tocopherols and Citric Acid), Brewers Dried Yeast, Natural Chicken Flavor, Dried Egg Product, Oil Blend (Soybean oil, Olive Oil, Salmon Oil, Evening Primrose Oil; Preserved with Natural Mixed Tocopherols and Citric Acid), Pumpkin, Apples, Sweet Potatoes, Carrots, Spinach, Blueberries, Dried Cranberries, Clams, Whole Ground Flaxseed, Calcium Carbonate, Salt, Potassium Chloride, Taurine, Green Lipped Mussels, Dried Chicory Root Extract, Yucca schidigera extract, Grape Seed Extract, Dried Kelp, L-Ascorbyl-2-Polyphosphate, Glucosamine Hydrochloride, Chondroitin Sulfate, Vitamin E Supplement, Ferrous Sulfate, DL-Methionine, Zinc Proteinate, Zinc Oxide, Manganese Proteinate, Niacin, Copper Proteinate, Folic Acid, Vitamin B12 Supplement, Copper Sulfate, Manganous Oxide, Vitamin A Supplement, Sodium Selenite, Thiamine Mononitrat Calcium Pantothenate, Riboflavin, Pyridoxine Hydrochloride, Biotin, Vitamin D3 Supplement, Menadione Sodium Bisulfite Complex, Calcium Iodate, Dried Lactobacillus acidophilus Fermentation Solubles, Dried Lactobacillus lactis Fermentation Solubles, Dried lactobacillus casei Fermentation Solubles, Rosemary Extract.

创造了一种全新的生产模式：低温焗炉焗制，低温焗烤不仅保存了食物中的营养和矿物质，而且降低了油份，让您的宠物食得更健康。更值得一提的是，通过数年投入大量人力、财力经过不断的尝试，终于于2009年年初研制出适合猫咪的最好食粮，无论从口感、成分配比，营养分析等综合因素，是您可以选择的最好的食材。鸡肉，鸡肉粉，全糙米，全地面大麦，鸡肝，豌豆纤维，鸡脂肪（天然保存溷合生育酚和柠檬酸），干酵母，天然鸡肉香精，干蛋产品，溷合油（豆油，橄榄油，三文鱼油，月见草油，天然溷合生育酚和柠檬酸保存），南瓜，苹果，红薯，胡萝卜，菠菜，蓝莓，小红莓干，蛤，全亚麻籽，碳酸钙，盐，氯化钾，牛磺酸，绿唇贻贝，干菊苣根提取物，丝兰提取物，葡萄籽提取物，干海带，L -抗坏血酸- 2 -聚，氨基葡萄糖盐酸盐，硫酸软骨素，维生素E补充，硫酸亚铁，DL -蛋氨酸，锌蛋白盐，氧化锌，锰蛋白盐，烟酸，铜蛋白盐，叶酸，维生素B12，硫酸铜，氧化锰，维生素A补充剂，亚硒酸钠，硫胺Mononitrat泛酸钙，核黄素，盐酸吡哆醇，生物素，维生素D3的补充，亚硫酸氢钠甲萘醌复合，碘酸钙，干嗜酸乳杆菌发酵米糠，干乳酸乳杆菌萃取物，干乳杆菌萃取物，迷迭香提取物。

Methods Paraffin-embedded tissue blocks from 73 cases of NPC and 20 cases of chronic nasopharyngitis were used in this study. Using terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling and immunohisytochemistry S-P method, the apoptotic rate and expression of p16 and bcl-2 protein were examined.

采用TdT酶介导的生物素化dutp缺口末端标记技术和免疫组化S-P法，分别检测20例正常鼻咽粘膜组织及73 例NPC组织的细胞凋亡率和p16、bcl-2基因的表达。

Methods The expression of CD147 and MMP-2 proteins were detected by immunohistochemical method in 46 cases of gliomas, and compared with those in 14 cases of normal brain tissues. The correlation between them was analyzed.

应用免疫组织化学链霉素抗生物素蛋白－过氧化酶连接法，检测46例胶质瘤瘤组织中CD147蛋白及MMP-2蛋白的表达，分别与正常脑组织14例进行对照。

Methods MMP-2 and MMP-9 were examined immunohistochemically in TCCB and 5 normal bladder tissue. The correlation between the expression of MMP-2 and MMP-9 and tumor grade and stage was analysed.

应用链霉菌抗生物素蛋白－过氧化酶免疫组化方法，检测5例正常膀胱组织和49例膀胱移行细胞癌组织中MMP-2和MMP-9的表达水平。

Initial Investigation into Relationship between Vocalization Habits and Singing;2. The effects of electrically stimuating the nucleus robustus archistriatalis and RA cup on vocalization and respiration were observed in the oscine bird,Carduelis Spinus.

电刺激鸣禽黄雀古纹状体粗核和RA壳区，再用生物素标记的葡聚糖胺示踪剂对其纤维联系进行研究，以了解RA及其壳区对发声和呼吸的影响及其神经支配。

Vitamin A, carotene, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K , folacin, calcium pantothenate, niacinamide, biotin, magnesia, ferrous gluconate, zinc gluconate, Calcium Gluconate, Sodium Selenite.

维生素A，-胡萝卜素，维生素B1，维生素B2，维生素B6，维生素B12，维生素C，维生素D，维生素E，维生素k，叶酸，泛酸钙，烟酰胺，生物素，氧化镁，葡萄糖酸亚铁，葡萄糖酸钙，葡萄糖酸锌，亚硒酸钠。

Chicken meal, brown rice, rice, corn, corn gluten meal, chicken fat, chicken, natural chicken flavor, pea fiber, rice flour, rice hulls, wheat gluten, dried beet pulp, soya oil, sodium silico aluminate, dried brewers yeast, fructo-oligosaccharides, anchovy oil (source of EPA/DHA), calcium sulfate, salt, potassium chloride, dried egg product, choline chloride, DL-methionine, taurine, Vitamins [DL-alpha tocopherol acetate, L-ascorbyl-2-polyphosphate, niacin supplement, biotin, riboflavin (vitamin B2) supplement, D-calcium pantothenate, vitamin A acetate, pyridoxine hydrochloride (vitamin B6), thiamine mononitrate (vitamin B1), folic acid, vitamin B12 supplement, vitamin D3 supplement], sodium tripolyphosphate, tea, Trace Minerals , L-Carnitine, preserved with natural mixed tocopherols and citric acid, rosemary extract.

能看懂的最好，我用翻译软件翻译一下是这样的鸡肉粉，大米，鸡肉脂肪，玉米蛋白粉，鸡肉，玉米，天然鸡味，干蛋产品，干甜菜粕，干燥啤酒酵母，豆油，凤尾鱼油（来源的EPA / DHA的）， 1赖氨酸，纤维素粉，盐，氯化钾，钠硅铝酸盐，果糖低聚糖，干啤酒酵母提取物，氯化胆碱，牛磺酸，维生素[消旋-α生育酚醋酸酯， L -抗坏血酸- 2 -聚，生物素，烟酸的补充，核黄素（维生素B2 ）的补充， D -泛酸钙，盐酸吡哆醇（维生素B6 ），维生素A醋酸酯，维生素B1单（维生素B1 ），叶酸酸，维生素B12的补充，补充维生素D3 ] ，微量矿物，万寿菊提取物， L -肉碱，保存与自然混合生育酚和柠檬酸，迷迭香提取物

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。