生物电

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The crystal structures, photophysical and electrochemical properties of thesecomplexes have been further studied in detail.

本文合成了几个系列的多吡啶化合物及其部分金属配合物并研究了它们的光、电、磁学性质及生物活性等。

In particular, it is unknown how the relevant neural substrates function as a network to mediate perspective-taking during the task requiring understanding other's belief. The current work first developed a new psychological paradigm that excludes potential confounds such as the processing of language, biological motion, episodic memory and causal coherence in the paradigms used in previous studies. I recorded neural activities associated with mental inference task using functional magnetic resonance imaging and electroencephalography in order to investigate the functional roles of several brain areas (i.e. precuneus, MPFC, etc.) that engage in understanding other's belief. I also proposed a computational model based on the neuroimaging results that simulated the neurocognitive mechanism underlying cortical dynamics in correct judgment of other's belief.

本文首先发展了一个新的心理学实验范式，排除以往常用&错误信念&范式中与语言加工、生物运动、情景记忆及事件连续性和因果性等方面的混淆，采用功能磁共振成像（functional magnetic resonance imaging， fMRI）和脑电（Electroencephalography， EEG）时频分析等脑成像技术，记录与理解他人信念任务相关的神经活动，通过对行为和脑成像数据的分析，研究楔前叶和内侧前额叶等脑区在理解他人信念任务中的功能意义，并根据脑成像结果，提出一个基于自我和他人视角竞争的计算模型，模拟大脑在理解他人信念时做出正确决策时的认知神经机制。

The biologic unit of material life is the protoplasmic cell, the communal association of chemical, electrical, and other basic energies.

物质生命的生物单元是原生质细胞、化学、电和其它基本能量联合构成。

Radicals, hydrogen peroxid e and singlet oxygen are known to oxidize biological macromolecules, with

O2 -。1O2 是一种反应活性相当高的亲电瞬态中间体，能高效地氧化生物大分子，造成生物体的

A biomorphic carbon retaining its anatomical feature was prepared by carbonizing wood through controlling heating rates.

中文摘要：利用热解法制备了具有一定生物形态的多孔碳材料，并采用电沉积方法在所得碳材料表面沉积贵金属铂微粒。

However, the highly conductive electrode material on the surface of the body coerces the contact surface underneath the electrode to be changed into an equal potential This reduces the current density at the interior of the body and affects the distribution of the sens ing field.

但是，导电材料的电极将其下的被测生物体表面强制为等电势，从而降低生物体内的电流分布密度，影响场域内部的电场分布。

This product take the electric appliance titanium as a core,makes the high-tech product with the nanometer biology Functional Materials union,has the thermoelectricity and the piezoelectricity dual effects,forms the resonating sympathetic chord with the human body magnetic field,may release the heat energy,the anoin,the far infrared effect improvement blood circulation continuously,activation cell promotion metabolism,dredges the path,relieves sickness dehumidifies,the analgesia dispels coldly,enhancement organism disease-resistant ability.

自发热产品以电器钛为核心，与纳米生物功能材料结合制成的高新技术产品，具有的热电和压电的双重效果，与人体磁声形成共振共鸣，可连续释放热能、负离子、远红外产生深度热效应，并通过人体自身能量有效改善血液循环，激活细胞促进新陈代谢，疏通经络，祛风除湿，镇痛祛寒，增强机体抗病能力。

This main goal of this research is to design and implement a bioimpedance measurement system with multi-frequency band using LabVIEW for acupuncture point verification, provides the electrical impedance measurement and analysis data of acupuncture point, extrapolates various internal organs the activity and its disease in.

本研究主要的目的是设计一套以LabVIEW为控制介面之针灸点验证用多频生物阻抗量测仪，提供穴位电阻抗量测与分析数据，来推测各脏腑的活动和其疾病的所在。

This paper proposes a new EEG lossless compression technology based on improved DM Coding and Adaptive 2-Value Arithmetic Coding:First, DM Coding is used to the original data, and them through an appropriate transform of data format, most of the "redundant" bits in every byte are removed, also its Entropy of bits is reduced.

引言 多年来，嫡编码作为数据无损压缩的重要手段在生物医学信号的处理中一直得到广泛地应用，同时对于脑电信号的处理来说更是如此：由于神经疾病的诊断需要容量庞大的分析数据以及脑电波自身内涵的复杂性使得无损压缩对于脑电数据的处理而言异常重要；在另一方面，霍夫曼编码(Huffman Cod-ing)。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。